Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAG685_RS23520 Genome accession   NZ_CP152345
Coordinates   5150269..5151969 (+) Length   566 a.a.
NCBI ID   WP_406665187.1    Uniprot ID   -
Organism   Pseudomonas indoloxydans strain SDU_PSEU1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5145269..5156969
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAG685_RS23485 nadC 5145762..5146610 (+) 849 WP_406665173.1 carboxylating nicotinate-nucleotide diphosphorylase -
  AAG685_RS23490 - 5146737..5147648 (-) 912 WP_406665175.1 ABC transporter ATP-binding protein -
  AAG685_RS23495 - 5147645..5148493 (-) 849 WP_406665177.1 hypothetical protein -
  AAG685_RS23500 - 5148490..5148702 (-) 213 WP_406665179.1 hypothetical protein -
  AAG685_RS23505 - 5148710..5149306 (-) 597 WP_406665181.1 ABC transporter permease -
  AAG685_RS23510 - 5149415..5149849 (-) 435 WP_406665183.1 hypothetical protein -
  AAG685_RS23515 pilA 5149855..5150040 (-) 186 WP_406665185.1 type IV pilin protein Machinery gene
  AAG685_RS23520 pilB 5150269..5151969 (+) 1701 WP_406665187.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAG685_RS23525 pilC 5151973..5153193 (+) 1221 WP_406665189.1 type II secretion system F family protein Machinery gene
  AAG685_RS23530 pilD 5153198..5154064 (+) 867 WP_406665191.1 prepilin peptidase Machinery gene
  AAG685_RS23535 coaE 5154266..5154874 (+) 609 WP_406665193.1 dephospho-CoA kinase -
  AAG685_RS23540 yacG 5154871..5155065 (+) 195 WP_406665195.1 DNA gyrase inhibitor YacG -
  AAG685_RS23545 - 5155154..5155369 (-) 216 WP_406665197.1 hypothetical protein -
  AAG685_RS23550 - 5155474..5156163 (-) 690 WP_406665199.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62179.08 Da        Isoelectric Point: 5.4357

>NTDB_id=988541 AAG685_RS23520 WP_406665187.1 5150269..5151969(+) (pilB) [Pseudomonas indoloxydans strain SDU_PSEU1]
MSDTAQLTGLARQLVNASLLDERTASQAFAQALRNKIPLVSYLVQNKLASSRAVAEIAAEQFGVPYFDLSAIDKEHQPRE
IISEKLCRQHRVLPLYRRGNKLFIALSDPTNHQAITDVQFSTGMSTEALLVEDDKLGDALDKFFDTGTSGMEDLADVDLD
GLDVEAVSDEADDAGAGGDADDAPVVRFINKMLLDAIKGGSSDLHFEPYEKAYRVRFRTDGMLHEVARPPIQLASRLSAR
LKVMAQLDISERRRPQDGRIKMKVSKTKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEIQKELYLTALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPKHGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSCKKEVTIPHDALLKEGFPE
EKIGSFKLYAANGCDNCKGGYKGRVGIYEVVKITPALQRIIMEEGNSIEIAEQARADGFNDLRTSGLLKAMHGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=988541 AAG685_RS23520 WP_406665187.1 5150269..5151969(+) (pilB) [Pseudomonas indoloxydans strain SDU_PSEU1]
ATGAGCGATACCGCCCAACTCACCGGCCTCGCCCGCCAACTGGTCAATGCCAGCCTTCTCGACGAACGCACCGCCAGCCA
GGCCTTCGCCCAGGCGCTGCGCAACAAGATTCCCCTGGTCTCCTACCTGGTACAGAACAAGCTGGCCTCCAGCCGTGCCG
TGGCCGAGATCGCCGCCGAGCAGTTCGGCGTGCCCTACTTCGATCTCAGCGCCATCGACAAAGAGCATCAGCCGAGAGAG
ATCATCAGCGAAAAACTGTGCCGCCAGCATCGTGTGCTGCCGCTCTATCGCCGTGGCAACAAGCTGTTCATCGCCCTCTC
CGACCCCACCAATCACCAGGCCATCACCGATGTGCAGTTCAGCACCGGTATGAGCACCGAAGCGCTGCTGGTCGAGGACG
ACAAACTGGGCGACGCGCTGGACAAGTTCTTCGACACCGGCACCAGCGGCATGGAAGACCTGGCCGATGTCGACCTGGAT
GGTCTCGACGTGGAGGCGGTCAGTGATGAGGCAGACGATGCAGGCGCCGGCGGTGATGCCGACGATGCACCGGTGGTGCG
CTTCATCAACAAGATGCTGCTCGACGCCATCAAGGGCGGCTCTTCCGACCTGCACTTCGAGCCTTACGAAAAGGCCTACC
GCGTACGCTTTCGTACCGATGGCATGCTGCATGAGGTGGCACGCCCACCCATCCAGTTGGCCAGTCGCCTTTCAGCCCGC
CTGAAGGTCATGGCCCAGCTGGACATTTCCGAGCGACGCCGGCCGCAGGACGGCCGGATCAAGATGAAGGTGTCGAAGAC
CAAGGCCATCGACTTTCGCGTCAACACCCTGCCCACCCTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCTACCA
GCGCGCAGATGGGCATCGATGCCCTGGGTTATGAAGAGATACAGAAGGAACTCTACCTGACGGCGCTCAAGCAGCCGCAA
GGCATGATCCTGGTGACGGGCCCGACCGGTTCCGGCAAGACGGTATCGCTGTACACCGGCCTGAATATCCTCAACACCCC
CGACATCAATATTTCCACGGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCCAAGC
ACGGCATGGACTTCTCCCAGGCCCTGCGCGCCTTCCTGCGCCAGGATCCGGACGTGATCATGGTGGGCGAGATCCGCGAC
CTGGAAACCGCCGAAATCGCCATCAAGGCGGCCCAGACCGGGCACATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
GGAAACCCTCACTCGTCTGCGCAACATGGGTGTGCCGGCCTTCAACATCGCCACCTCGGTGAACCTGATCATCGCCCAGC
GCCTGGCGCGCAAACTCTGCAGTTGCAAGAAGGAAGTGACGATTCCCCACGATGCCTTGCTGAAAGAAGGCTTTCCCGAA
GAAAAGATCGGTTCGTTCAAGCTTTATGCAGCCAACGGCTGTGACAATTGCAAAGGCGGCTATAAAGGAAGGGTGGGGAT
TTATGAAGTAGTTAAAATCACGCCCGCGCTGCAACGCATTATCATGGAAGAAGGCAACTCCATCGAAATCGCTGAACAGG
CGAGGGCCGACGGCTTCAACGATCTCCGTACTTCCGGGCTGCTCAAGGCGATGCATGGCATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.596

100

0.59
  pilB Acinetobacter baylyi ADP1

58.333

99.647

0.581
  pilB Legionella pneumophila strain ERS1305867

54.77

100

0.548
  pilF Neisseria gonorrhoeae MS11

50.263

100

0.507
  pilB Vibrio cholerae strain A1552

54.118

90.106

0.488
  pilB Vibrio campbellii strain DS40M4

48.668

99.47

0.484
  pilB Vibrio parahaemolyticus RIMD 2210633

51.024

94.876

0.484
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.848

93.11

0.371
  pilF Thermus thermophilus HB27

36.945

99.47

0.367

Multiple sequence alignment