Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   B2D37_RS23985 Genome accession   NZ_CP019947
Coordinates   2976215..2976400 (-) Length   61 a.a.
NCBI ID   WP_079203185.1    Uniprot ID   -
Organism   Pseudomonas sp. CC6-YY-74     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2971215..2981400
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2D37_RS13570 - 2972851..2973792 (-) 942 WP_079203183.1 ABC transporter ATP-binding protein -
  B2D37_RS13575 - 2973789..2974628 (-) 840 WP_079203184.1 hypothetical protein -
  B2D37_RS13580 - 2974630..2975439 (-) 810 WP_256573079.1 ABC transporter permease subunit -
  B2D37_RS23120 - 2975742..2976200 (-) 459 WP_154067090.1 hypothetical protein -
  B2D37_RS23985 pilA 2976215..2976400 (-) 186 WP_079203185.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  B2D37_RS13590 pilB 2976633..2978336 (+) 1704 WP_079203186.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B2D37_RS13595 pilC 2978340..2979560 (+) 1221 WP_079203187.1 type II secretion system F family protein Machinery gene
  B2D37_RS13600 pilD 2979564..2980433 (+) 870 WP_154067091.1 A24 family peptidase Machinery gene

Sequence


Protein


Download         Length: 61 a.a.        Molecular weight: 6671.00 Da        Isoelectric Point: 10.5719

>NTDB_id=219102 B2D37_RS23985 WP_079203185.1 2976215..2976400(-) (pilA) [Pseudomonas sp. CC6-YY-74]
MKSLKKQKGFTLIELMIVIAIIGILAAIAIPQFNDYRAKANDSLALADNKNMVTSFSAAMR

Nucleotide


Download         Length: 186 bp        

>NTDB_id=219102 B2D37_RS23985 WP_079203185.1 2976215..2976400(-) (pilA) [Pseudomonas sp. CC6-YY-74]
ATGAAGTCTCTCAAGAAACAAAAAGGTTTTACCCTGATCGAACTGATGATCGTGATTGCAATTATCGGGATTTTGGCTGC
TATCGCTATTCCGCAGTTTAATGACTACCGCGCGAAAGCGAATGACTCCCTGGCTCTTGCTGACAACAAGAATATGGTTA
CGTCGTTTTCTGCAGCAATGCGATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

54.545

100

0.59

  pilA2 Legionella pneumophila str. Paris

61.818

90.164

0.557

  pilA2 Legionella pneumophila strain ERS1305867

61.818

90.164

0.557

  pilA/pilA1 Eikenella corrodens VA1

58.929

91.803

0.541

  pilA Pseudomonas aeruginosa PAK

57.895

93.443

0.541

  comP Acinetobacter baylyi ADP1

61.111

88.525

0.541

  pilE Neisseria gonorrhoeae strain FA1090

58.491

86.885

0.508

  pilE Neisseria gonorrhoeae MS11

58.491

86.885

0.508

  pilA Acinetobacter baumannii strain A118

51.724

95.082

0.492

  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

57.692

85.246

0.492

  pilA/pilAI Pseudomonas stutzeri DSM 10701

69.048

68.852

0.475

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

72.5

65.574

0.475

  pilA Acinetobacter nosocomialis M2

65.909

72.131

0.475

  pilA Vibrio campbellii strain DS40M4

77.778

59.016

0.459

  pilA Vibrio parahaemolyticus RIMD 2210633

56

81.967

0.459

  pilA Vibrio cholerae strain A1552

63.636

72.131

0.459

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

63.636

72.131

0.459

  pilA Vibrio cholerae C6706

63.636

72.131

0.459

  pilA/pilAII Pseudomonas stutzeri DSM 10701

55.102

80.328

0.443

  pilA/pilA4 Thermus thermophilus HB27

56.818

72.131

0.41

  pilA Haemophilus influenzae 86-028NP

73.529

55.738

0.41

  pilA Haemophilus influenzae Rd KW20

73.529

55.738

0.41

  comF Acinetobacter baylyi ADP1

60

65.574

0.393

  pilA Glaesserella parasuis strain SC1401

72.727

54.098

0.393

  comE Acinetobacter baylyi ADP1

50

75.41

0.377

  comGD Latilactobacillus sakei subsp. sakei 23K

41.071

91.803

0.377

  pilX Neisseria meningitidis 8013

57.895

62.295

0.361

  pilL Neisseria gonorrhoeae MS11

57.895

62.295

0.361

  pilY2 Acinetobacter baumannii D1279779

48.889

73.77

0.361


Multiple sequence alignment