Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B2D37_RS13590 Genome accession   NZ_CP019947
Coordinates   2976633..2978336 (+) Length   567 a.a.
NCBI ID   WP_079203186.1    Uniprot ID   -
Organism   Pseudomonas sp. CC6-YY-74     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2971633..2983336
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B2D37_RS13570 - 2972851..2973792 (-) 942 WP_079203183.1 ABC transporter ATP-binding protein -
  B2D37_RS13575 - 2973789..2974628 (-) 840 WP_079203184.1 hypothetical protein -
  B2D37_RS13580 - 2974630..2975439 (-) 810 WP_256573079.1 ABC transporter permease subunit -
  B2D37_RS23120 - 2975742..2976200 (-) 459 WP_154067090.1 hypothetical protein -
  B2D37_RS23985 pilA 2976215..2976400 (-) 186 WP_079203185.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  B2D37_RS13590 pilB 2976633..2978336 (+) 1704 WP_079203186.1 type IV-A pilus assembly ATPase PilB Machinery gene
  B2D37_RS13595 pilC 2978340..2979560 (+) 1221 WP_079203187.1 type II secretion system F family protein Machinery gene
  B2D37_RS13600 pilD 2979564..2980433 (+) 870 WP_154067091.1 A24 family peptidase Machinery gene
  B2D37_RS13605 coaE 2981993..2982601 (+) 609 WP_079203188.1 dephospho-CoA kinase -
  B2D37_RS13610 yacG 2982598..2982804 (+) 207 WP_079203189.1 DNA gyrase inhibitor YacG -
  B2D37_RS13615 - 2982887..2983102 (-) 216 WP_079203190.1 hypothetical protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62114.09 Da        Isoelectric Point: 5.4482

>NTDB_id=219083 B2D37_RS13590 WP_079203186.1 2976633..2978336(+) (pilB) [Pseudomonas sp. CC6-YY-74]
MNEAPNLTGLARQLVNAELLDERAAAQAQAQALRNKIPLVSYLVQNKLASSRAVAEIASEQFGVPLCDLKAIDKDSQPHD
LVSEKLCRQHRVLPLYRRGNKLFVALSDPTNHQAVTDIQFSTGLSTEALLVEDDKLGDAIDKFFEAANSGMDDLGDVDLD
GLDVQAVDDNKEEGVAGESADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKAYRVRFRTDGILQEIARPPIQLAPRISAR
LKVMAALDISERRKPQDGRIKMRISKTKSIDFRVSTCPTLWGEKIVMRILDPSSAQMGIDALGYEPGQKDLYMAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFTAALRAFLRQDPDIIMVGEIRD
LDTASIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVSLIIAQRLARKLCSHCKKEVQIPREALLEEGFP
QDQLGSFKIYGPVGCENCNGGYKGRIGIYEVVKNTPALQRIIMEEGNSIDISVQMRKDGFNDLRTSALLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=219083 B2D37_RS13590 WP_079203186.1 2976633..2978336(+) (pilB) [Pseudomonas sp. CC6-YY-74]
ATGAACGAAGCTCCGAATCTGACCGGCCTGGCTCGACAACTGGTCAACGCCGAACTGCTGGACGAACGCGCCGCGGCCCA
GGCCCAGGCCCAGGCGCTGCGTAACAAGATCCCGCTGGTGTCCTACCTGGTGCAGAACAAGCTGGCCAGCAGCCGGGCAG
TGGCGGAAATCGCCTCCGAGCAGTTTGGCGTGCCGCTGTGCGACCTCAAGGCCATCGACAAGGACAGCCAGCCGCACGAC
CTGGTCAGCGAGAAGCTGTGTCGCCAGCACCGCGTGCTGCCGCTGTACCGGCGTGGCAACAAGCTGTTCGTCGCCCTGTC
CGACCCGACCAACCACCAGGCAGTGACCGATATCCAGTTCAGCACCGGACTGAGTACCGAAGCGCTGCTGGTGGAGGACG
ACAAGCTTGGGGATGCCATTGACAAGTTCTTCGAGGCGGCCAACAGCGGCATGGATGACCTGGGCGACGTCGACCTCGAC
GGCCTCGACGTGCAGGCGGTGGATGACAACAAGGAAGAGGGGGTCGCCGGCGAAAGTGCCGACGACGCGCCGGTGGTGCG
CTTCGTCAACAAGATGCTGCTGGACGCGATCAAGGGCGGCTCCTCGGACTTGCACTTCGAGCCGTACGAGAAGGCCTACC
GGGTGCGCTTTCGTACCGACGGCATTCTGCAGGAGATCGCCCGGCCGCCGATCCAACTGGCACCGCGGATCTCGGCACGC
CTGAAGGTGATGGCCGCGCTGGATATTTCCGAGCGGCGCAAACCGCAGGATGGGCGGATCAAGATGCGCATCTCGAAGAC
CAAGTCGATCGACTTTCGCGTCAGCACCTGCCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCCTCTA
GCGCACAGATGGGCATCGATGCGCTGGGTTATGAGCCTGGGCAGAAAGACCTGTACATGGCGGCGCTCAAACAACCGCAA
GGCATGATCCTGGTGACCGGACCGACCGGCTCGGGCAAGACGGTGTCGCTGTACACCGGTCTGAACATCCTCAATACCGT
GGACGTGAATATCTCCACCGCCGAAGACCCGGTGGAAATCAACCTGGAGGGTATCAACCAGGTCAACGTCAACCCCAAGC
AGGGCATGGACTTCACCGCCGCGCTGCGCGCCTTCCTGCGTCAGGACCCGGACATCATCATGGTCGGCGAAATCCGCGAC
CTGGATACCGCCTCCATCGCGATCAAGGCGGCGCAGACCGGGCACATGGTGATGTCGACCCTGCACACCAACAGCGCCGC
GGAAACCCTGACGCGTTTGCGCAACATGGGCGTGCCCTCGTTCAACATCGCCACCTCGGTCAGCCTGATCATTGCCCAGC
GCCTGGCGCGCAAATTGTGCAGCCACTGCAAGAAGGAAGTGCAGATCCCGCGCGAGGCCCTGCTCGAGGAAGGCTTCCCG
CAAGACCAGCTCGGCAGTTTCAAGATTTACGGCCCGGTCGGCTGCGAAAATTGCAACGGCGGTTACAAGGGCCGTATCGG
AATTTATGAAGTGGTTAAAAACACGCCTGCATTGCAACGGATTATCATGGAGGAAGGTAATTCCATTGATATCTCCGTGC
AAATGCGCAAAGACGGCTTCAATGACCTGCGTACCTCGGCCTTGCTCAAGGCCATGCAGGGCATCACCAGCCTCGAAGAA
GTGAACCGCGTGACAAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.957

100

0.598
  pilB Acinetobacter baylyi ADP1

58.053

99.647

0.578
  pilB Legionella pneumophila strain ERS1305867

55.342

100

0.557
  pilB Vibrio cholerae strain A1552

50

99.118

0.496
  pilF Neisseria gonorrhoeae MS11

49.47

99.824

0.494
  pilB Vibrio parahaemolyticus RIMD 2210633

51.306

94.533

0.485
  pilB Vibrio campbellii strain DS40M4

48.582

99.471

0.483

Multiple sequence alignment