Detailed information NTG1095982

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: ACMYQ0_RS13965
• Genome accession: NZ_CP180477
• Coordinates: 3002125..3002310 (-)
• Length: 61 a.a.
• NCBI ID: WP_415753510.1
• Uniprot ID: -
• Organism: Pseudomonas leptonychotis strain CCM 8849
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2998652..3007570	3002125..3002310	within	0

Gene organization within MGE regions

Location: 2998652..3007570

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMYQ0_RS13940	-	2998652..2999551 (-)	900	WP_415753505.1	ABC transporter ATP-binding protein	-
ACMYQ0_RS13945	-	2999554..3000387 (-)	834	WP_415753506.1	hypothetical protein	-
ACMYQ0_RS13950	-	3000389..3001198 (-)	810	WP_415753507.1	hypothetical protein	-
ACMYQ0_RS13955	-	3001302..3001535 (-)	234	WP_415753508.1	type IV pilin protein	-
ACMYQ0_RS13960	-	3001633..3002109 (-)	477	WP_415753509.1	hypothetical protein	-
ACMYQ0_RS13965	pilA	3002125..3002310 (-)	186	WP_415753510.1	type IV pilin protein	Machinery gene
ACMYQ0_RS13970	pilB	3002544..3004247 (+)	1704	WP_415753511.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACMYQ0_RS13975	pilC	3004250..3005470 (+)	1221	WP_415753512.1	type II secretion system F family protein	Machinery gene
ACMYQ0_RS13980	pilD	3005477..3006343 (+)	867	WP_415753513.1	prepilin peptidase	Machinery gene
ACMYQ0_RS13985	coaE	3006500..3007114 (+)	615	WP_415753514.1	dephospho-CoA kinase	-
ACMYQ0_RS13990	yacG	3007111..3007311 (+)	201	WP_415753515.1	DNA gyrase inhibitor YacG	-
ACMYQ0_RS13995	-	3007355..3007570 (-)	216	WP_415753516.1	hypothetical protein	-

Sequence

Protein

• Length: 61 a.a.
• Molecular weight: 6537.78 Da
• Isoelectric Point: 10.2806

>NTDB_id=1095982 ACMYQ0_RS13965 WP_415753510.1 3002125..3002310(-) (pilA) [Pseudomonas leptonychotis strain CCM 8849]
MKSLKKQKGFTLIELMIVVAIIGILAAIAIPQFNEYRAKSNDTVATADAKNAITTMASAQL

Nucleotide

• Length: 186 bp

>NTDB_id=1095982 ACMYQ0_RS13965 WP_415753510.1 3002125..3002310(-) (pilA) [Pseudomonas leptonychotis strain CCM 8849]
ATGAAGTCTCTCAAGAAGCAAAAAGGTTTTACCCTGATCGAGCTGATGATCGTTGTTGCGATTATTGGTATTTTGGCTGC
TATTGCTATCCCGCAGTTTAATGAGTATCGCGCCAAGTCGAACGACACTGTCGCTACTGCGGATGCGAAAAATGCAATTA
CCACTATGGCCTCGGCCCAGCTTTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (5-30)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Pseudomonas aeruginosa PAK	62.5	91.803	0.574
pilA	Vibrio parahaemolyticus RIMD 2210633	62	81.967	0.508
pilA	Acinetobacter baumannii strain A118	81.081	60.656	0.492
pilA2	Legionella pneumophila strain ERS1305867	65.217	75.41	0.492
pilA2	Legionella pneumophila str. Paris	65.217	75.41	0.492
pilA/pilAI	Pseudomonas stutzeri DSM 10701	69.048	68.852	0.475
pilA/pilA1	Eikenella corrodens VA1	70.732	67.213	0.475
pilA	Acinetobacter nosocomialis M2	78.378	60.656	0.475
pilA	Ralstonia pseudosolanacearum GMI1000	64.444	73.77	0.475
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	77.778	59.016	0.459
pilA	Vibrio cholerae strain A1552	77.778	59.016	0.459
pilA	Vibrio cholerae C6706	77.778	59.016	0.459
pilA/pilAII	Pseudomonas stutzeri DSM 10701	57.143	80.328	0.459
pilA/pilA4	Thermus thermophilus HB27	54	81.967	0.443
pilA	Vibrio campbellii strain DS40M4	75	59.016	0.443
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	67.5	65.574	0.443
pilE	Acinetobacter baumannii D1279779	47.273	90.164	0.426
pilV	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	55.319	77.049	0.426
comF	Acinetobacter baylyi ADP1	63.158	62.295	0.393
pilA/pilA1	Synechocystis sp. PCC 6803	42.857	91.803	0.393
pilE	Neisseria gonorrhoeae strain FA1090	67.647	55.738	0.377
pilE	Neisseria gonorrhoeae MS11	67.647	55.738	0.377
pilA	Haemophilus influenzae 86-028NP	67.647	55.738	0.377
pilA	Haemophilus influenzae Rd KW20	67.647	55.738	0.377
comE	Acinetobacter baylyi ADP1	62.162	60.656	0.377
pilA	Glaesserella parasuis strain SC1401	68.75	52.459	0.361
comGE	Staphylococcus aureus MW2	37.931	95.082	0.361
pilL	Neisseria gonorrhoeae MS11	62.857	57.377	0.361
pilE	Legionella pneumophila strain ERS1305867	41.509	86.885	0.361
pilE	Legionella pneumophila str. Paris	41.509	86.885	0.361