Detailed information NTG1095985

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: ACMYQ0_RS13980
• Genome accession: NZ_CP180477
• Coordinates: 3005477..3006343 (+)
• Length: 288 a.a.
• NCBI ID: WP_415753513.1
• Uniprot ID: -
• Organism: Pseudomonas leptonychotis strain CCM 8849
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	2998652..3007570	3005477..3006343	within	0

Gene organization within MGE regions

Location: 2998652..3007570

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ACMYQ0_RS13940	-	2998652..2999551 (-)	900	WP_415753505.1	ABC transporter ATP-binding protein	-
ACMYQ0_RS13945	-	2999554..3000387 (-)	834	WP_415753506.1	hypothetical protein	-
ACMYQ0_RS13950	-	3000389..3001198 (-)	810	WP_415753507.1	hypothetical protein	-
ACMYQ0_RS13955	-	3001302..3001535 (-)	234	WP_415753508.1	type IV pilin protein	-
ACMYQ0_RS13960	-	3001633..3002109 (-)	477	WP_415753509.1	hypothetical protein	-
ACMYQ0_RS13965	pilA	3002125..3002310 (-)	186	WP_415753510.1	type IV pilin protein	Machinery gene
ACMYQ0_RS13970	pilB	3002544..3004247 (+)	1704	WP_415753511.1	type IV-A pilus assembly ATPase PilB	Machinery gene
ACMYQ0_RS13975	pilC	3004250..3005470 (+)	1221	WP_415753512.1	type II secretion system F family protein	Machinery gene
ACMYQ0_RS13980	pilD	3005477..3006343 (+)	867	WP_415753513.1	prepilin peptidase	Machinery gene
ACMYQ0_RS13985	coaE	3006500..3007114 (+)	615	WP_415753514.1	dephospho-CoA kinase	-
ACMYQ0_RS13990	yacG	3007111..3007311 (+)	201	WP_415753515.1	DNA gyrase inhibitor YacG	-
ACMYQ0_RS13995	-	3007355..3007570 (-)	216	WP_415753516.1	hypothetical protein	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31767.66 Da
• Isoelectric Point: 6.4673

>NTDB_id=1095985 ACMYQ0_RS13980 WP_415753513.1 3005477..3006343(+) (pilD) [Pseudomonas leptonychotis strain CCM 8849]
MNDLLAGNLPAFVCVSLLLGLLIGSFLNVVVYRLPKMMQRDWREQAREILELPAEAQAATFNLVLPNSSCPHCAHEIKPW
ENIPVISYLFLRGKCSSCKAPISPRYPLVELTCGLLSAYIAWHFGFTWQAGGMLLLTWGLLAMSLIDADHQLLPDSLVLP
LLWLGLIANNFGLFTSLEDALWGAIAGYLSLWSVYWLFKLVTGKEGMGYGDFKLLAMLGAWGGWQVIPLTILLSSLVGAV
LGLLMLRLRNAETSTPIPFGPYLAIAGWIALLWGEQITASYLQIAGFR

Nucleotide

• Length: 867 bp

>NTDB_id=1095985 ACMYQ0_RS13980 WP_415753513.1 3005477..3006343(+) (pilD) [Pseudomonas leptonychotis strain CCM 8849]
ATGAACGACCTACTGGCCGGCAATTTGCCGGCCTTTGTTTGTGTATCCCTGTTGCTGGGCTTGCTGATTGGTAGCTTCCT
CAATGTAGTGGTCTACCGCCTACCAAAAATGATGCAGCGCGACTGGCGTGAACAGGCTCGCGAGATATTGGAGTTACCAG
CCGAAGCCCAGGCAGCAACATTCAACCTGGTACTGCCAAACTCAAGCTGCCCGCACTGCGCCCATGAAATAAAGCCTTGG
GAGAACATCCCGGTTATTAGCTATCTGTTTCTGCGCGGTAAATGCTCAAGCTGTAAAGCACCGATTAGCCCGCGCTATCC
ACTGGTTGAGCTGACCTGCGGACTATTGTCCGCTTACATTGCCTGGCACTTTGGTTTTACCTGGCAAGCCGGCGGCATGC
TGCTGTTGACCTGGGGTTTGCTGGCGATGAGCCTAATCGATGCTGATCACCAACTGCTACCGGACTCACTGGTACTGCCG
TTACTGTGGCTCGGTTTAATCGCCAATAACTTCGGCCTGTTTACCAGCTTGGAAGATGCACTCTGGGGCGCAATTGCCGG
CTACTTGAGCCTGTGGTCGGTGTACTGGCTGTTCAAGCTGGTAACCGGCAAGGAAGGCATGGGCTATGGTGACTTCAAGC
TGCTGGCCATGCTCGGCGCGTGGGGTGGTTGGCAGGTAATACCGCTGACGATATTGCTGTCATCACTGGTAGGCGCTGTA
CTCGGCCTACTCATGCTGCGCCTGCGTAACGCTGAAACCAGCACACCTATTCCTTTCGGCCCTTACCTGGCGATTGCCGG
CTGGATTGCCTTGCTCTGGGGTGAGCAAATCACCGCCAGCTACCTGCAGATTGCCGGCTTCCGCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-243)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (18-124)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	52.431	100	0.524
pilD	Vibrio cholerae strain A1552	54.945	94.792	0.521
pilD	Acinetobacter nosocomialis M2	51.22	99.653	0.51
pilD	Vibrio campbellii strain DS40M4	53.846	94.792	0.51
pilD	Neisseria gonorrhoeae MS11	49.071	93.403	0.458