Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ACMYQ0_RS13970 Genome accession   NZ_CP180477
Coordinates   3002544..3004247 (+) Length   567 a.a.
NCBI ID   WP_415753511.1    Uniprot ID   -
Organism   Pseudomonas leptonychotis strain CCM 8849     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 2998652..3007570 3002544..3004247 within 0


Gene organization within MGE regions


Location: 2998652..3007570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACMYQ0_RS13940 - 2998652..2999551 (-) 900 WP_415753505.1 ABC transporter ATP-binding protein -
  ACMYQ0_RS13945 - 2999554..3000387 (-) 834 WP_415753506.1 hypothetical protein -
  ACMYQ0_RS13950 - 3000389..3001198 (-) 810 WP_415753507.1 hypothetical protein -
  ACMYQ0_RS13955 - 3001302..3001535 (-) 234 WP_415753508.1 type IV pilin protein -
  ACMYQ0_RS13960 - 3001633..3002109 (-) 477 WP_415753509.1 hypothetical protein -
  ACMYQ0_RS13965 pilA 3002125..3002310 (-) 186 WP_415753510.1 type IV pilin protein Machinery gene
  ACMYQ0_RS13970 pilB 3002544..3004247 (+) 1704 WP_415753511.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ACMYQ0_RS13975 pilC 3004250..3005470 (+) 1221 WP_415753512.1 type II secretion system F family protein Machinery gene
  ACMYQ0_RS13980 pilD 3005477..3006343 (+) 867 WP_415753513.1 prepilin peptidase Machinery gene
  ACMYQ0_RS13985 coaE 3006500..3007114 (+) 615 WP_415753514.1 dephospho-CoA kinase -
  ACMYQ0_RS13990 yacG 3007111..3007311 (+) 201 WP_415753515.1 DNA gyrase inhibitor YacG -
  ACMYQ0_RS13995 - 3007355..3007570 (-) 216 WP_415753516.1 hypothetical protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62542.65 Da        Isoelectric Point: 5.4770

>NTDB_id=1095983 ACMYQ0_RS13970 WP_415753511.1 3002544..3004247(+) (pilB) [Pseudomonas leptonychotis strain CCM 8849]
MSEAPNLTGLARQLVNAELLSEKAAFQAQAQALRNKIPLVSYLVQNKLTTGKAVAEVAAEQFGVPYCDLNAMDKESQPRD
LISEKLIRQHRVIPLYRRGNKLFVGISDPTNHQAVTDVQFSTGLTTEPLLVEDDKLTDAIEKFFDSAANGMEEIGDIDLD
GVDIEAVDDDKQDINDGNSADDAPVVRFVHKMLLDAIKKGSSDLHFEPYEKTYRVRFRTDGILQEIARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMRISKSKSIDFRVSTCPTLWGEKIVMRILDSASAQMGIDALGYEEPQKELYMAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPKQGMDFTAALKSFLRQDPDIIMVGEIRD
LDTASIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCNNCRKEVKIPREALLEEGFP
ENKVDTIKLFGPVGCENCNGGYKGRVGIYEVVKNTPALQRIIMEEGNSIDISVQMRKDGFNDLRTSALLKAMQGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=1095983 ACMYQ0_RS13970 WP_415753511.1 3002544..3004247(+) (pilB) [Pseudomonas leptonychotis strain CCM 8849]
ATGAGCGAAGCTCCCAACCTAACTGGCTTAGCCAGACAACTGGTCAATGCCGAACTGCTTAGCGAAAAAGCCGCCTTCCA
AGCGCAAGCCCAAGCGCTGCGCAATAAAATCCCGCTGGTTTCCTATCTTGTCCAGAACAAACTCACGACAGGCAAAGCCG
TTGCTGAAGTGGCGGCCGAACAATTTGGCGTACCGTACTGCGACCTCAACGCCATGGATAAGGAAAGCCAGCCCCGCGAC
CTGATCAGCGAGAAGCTGATTCGCCAGCACCGGGTTATTCCGCTGTACCGCCGTGGAAACAAGTTATTTGTGGGGATTTC
TGACCCCACAAATCACCAGGCAGTTACCGACGTACAGTTCTCCACAGGCCTCACCACTGAACCGCTGCTAGTTGAAGACG
ACAAACTGACCGACGCTATCGAGAAGTTCTTTGACTCTGCCGCGAACGGGATGGAAGAAATAGGCGACATTGACCTCGAC
GGCGTGGATATTGAAGCCGTCGACGATGACAAGCAGGACATCAACGACGGCAACTCTGCTGACGACGCACCGGTTGTACG
CTTCGTGCACAAGATGCTTCTGGATGCGATCAAAAAAGGCTCATCTGACCTACACTTCGAGCCCTATGAAAAAACCTACC
GGGTACGCTTCCGCACCGACGGCATCTTGCAGGAAATAGCGCGCCCGCCCATTCAGCTCGCACCACGCATCTCCGCGCGC
CTCAAAGTCATGGCCGGCCTGGACATCTCCGAGCGTCGCAAGCCGCAGGATGGGCGCATCAAAATGCGCATATCCAAAAG
CAAATCCATCGACTTTCGCGTTAGCACTTGCCCAACCTTGTGGGGTGAGAAGATCGTAATGCGGATTTTGGATTCCGCCA
GCGCCCAGATGGGCATTGACGCCCTGGGTTATGAGGAGCCGCAGAAAGAACTCTACATGGCCGCCCTCAAACAACCACAA
GGCATGATTCTGGTAACCGGCCCTACCGGCTCCGGCAAAACCGTGTCCCTTTACACCGGCTTGAACATACTCAACACAGT
AGACGTCAACATCTCCACCGCCGAAGACCCTGTAGAAATCAACTTGGAAGGTATAAACCAGGTCAACGTTAATCCCAAGC
AGGGTATGGACTTTACCGCTGCGCTAAAATCCTTCCTGCGCCAGGATCCGGATATCATCATGGTCGGCGAGATTCGCGAC
CTGGACACCGCTTCTATTGCAGTTAAAGCCGCACAAACCGGCCACATGGTGATGTCAACGCTTCACACTAACAGCGCTGC
TGAGACCCTGACACGTCTTCGCAATATGGGCGTGCCTTCATTCAACATCGCCACCTCGGTCAACCTGATCATTGCTCAGC
GACTGGCACGCAAGCTCTGCAACAATTGCAGAAAAGAAGTAAAGATTCCCCGGGAGGCGCTTTTGGAAGAAGGTTTTCCA
GAAAACAAGGTCGACACGATCAAGCTTTTTGGCCCTGTGGGTTGTGAAAACTGCAACGGCGGCTATAAAGGCCGTGTCGG
AATATATGAAGTGGTTAAAAACACGCCTGCCCTGCAACGGATTATCATGGAGGAAGGCAATTCCATCGATATTTCCGTAC
AGATGCGCAAAGACGGCTTCAACGACCTGCGCACCTCCGCATTACTGAAAGCAATGCAGGGCATTACCAGCCTTGAAGAA
GTCAACCGAGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.793

100

0.582
  pilB Acinetobacter baylyi ADP1

57.168

99.647

0.57
  pilB Legionella pneumophila strain ERS1305867

55.07

100

0.556
  pilB Vibrio cholerae strain A1552

50.177

99.471

0.499
  pilB Vibrio campbellii strain DS40M4

48.763

99.824

0.487
  pilF Neisseria gonorrhoeae MS11

48.496

99.647

0.483
  pilB Vibrio parahaemolyticus RIMD 2210633

50.947

93.122

0.474
  pilF Thermus thermophilus HB27

38.868

93.474

0.363

Multiple sequence alignment