Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00790 experimental
T6SS type Type i1
Strain Vibrio cholerae V52
Replicon (RefSeq) chromosome (NZ_KQ410497)
Location 202021..225793
Function anti-bacterial; anti-eukaryotic; effector translocation; virulence; adherence
Comment Cytotoxicity towards Dictyostelium and cultured mammalian macrophages; inflammatory response, diarrhoea and actin cross-linking in mouse intestine; Killing of E. coli, Salmonella and Citrobacter rodentium.
References
[1] Pukatzki S et al (2006) Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system. Proc Natl Acad Sci U S A. 103(5):1528-33. [PudMed:16432199]
[2] Ma AT et al (2010) In vivo actin cross-linking induced by Vibrio cholerae type VI secretion system is associated with intestinal inflammation. Proc Natl Acad Sci U S A. 107(9):4365-70. [PudMed:20150509]
[3] Dong TG et al (2013) Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae. Proc Natl Acad Sci U S A . 110(7):2623-8. [PudMed:23362380]
[4] Ma AT et al (2009) Translocation of a Vibrio cholerae type VI secretion effector requires bacterial endocytosis by host cells. Cell Host Microbe. 5(3):234-43. [PudMed:19286133]
[5] Miyata ST et al (2011) Vibrio cholerae requires the type VI secretion system virulence factor VasX to kill Dictyostelium discoideum. Infect Immun. 79(7):2941-9. [PudMed:21555399]
[6] Shao Y et al (2014) Quorum regulatory small RNAs repress type VI secretion in Vibrio cholerae. Mol Microbiol. 92(5):921-30. [PudMed:24698180]
[7] Unterweger D et al (2014) The Vibrio cholerae type VI secretion system employs diverse effector modules for intraspecific competition. Nat Commun. 2.672916667. [PudMed:24686479]
[8] Borgeaud S et al (2015) Bacterial evolution. The type VI secretion system of Vibrio cholerae fosters horizontal gene transfer. Science. 347(6217):63-7. [PudMed:25554784]
[9] Stietz MS, Liang X, Li H, Zhang X, Dong TG. et al (2020) TssA-TssM-TagA interaction modulates type VI secretion system sheath-tube assembly in Vibrio cholerae.. Nat Commun. 11(1):5065. [PudMed:33033237]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentFHATssATssBTssCTssETssFTssGTssHTssITssJTssKTssLTssMVasH
Number12111111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
VCV52_RS00955 (rbsB) 197079..197957 - 879 WP_000737186.1 ribose ABC transporter substrate-binding protein RbsB
VCV52_RS00960 (rbsC) 198018..198986 - 969 WP_001997313.1 ribose ABC transporter permease
VCV52_RS00965 (rbsA) 199016..200518 - 1503 WP_000189455.1 ribose ABC transporter ATP-binding protein RbsA
VCV52_RS00970 (rbsD) 200545..200964 - 420 WP_001911632.1 D-ribose pyranase
VCV52_RS00980 201248..201508 + 261 WP_000799238.1 DUF3012 domain-containing protein
VCV52_RS00985 201656..202024 - 369 WP_001061295.1 hypothetical protein
T6CP011000 TssI VCV52_RS00990 (tssI) 202021..205074 - 3054 WP_000113305.1 type VI secretion system tip protein VgrG
T6CP011001 TssA VCV52_RS01000 205313..206575 - 1263 WP_000073086.1 ImpA family type VI secretion system protein
T6CP011002 TssM VCV52_RS01005 (tssM) 206626..210126 - 3501 WP_000225009.1 type VI secretion system membrane subunit TssM
T6CP011003 TssA VCV52_RS01010 (vasJ) 210187..211596 - 1410 WP_000426093.1 TssA family type VI secretion system protein VasJ
VCV52_RS01015 (tagO) 211606..212262 - 657 WP_001223375.1 type VI secretion system-associated protein TagO
T6CP012598 VasH VCV52_RS01020 (vasH) 212259..213851 - 1593 WP_000084802.1 sigma-54 dependent T6SS transcriptional regulator VasH
T6CP011004 TssH VCV52_RS01025 (tssH) 213854..216463 - 2610 WP_000619133.1 type VI secretion system ATPase TssH
T6CP011005 TssL VCV52_RS01030 216489..217262 - 774 WP_000083407.1 type IVB secretion system protein IcmH/DotU
T6CP011006 TssK VCV52_RS01035 (tssK) 217265..218599 - 1335 WP_000458007.1 type VI secretion system baseplate subunit TssK
T6CP011007 TssJ VCV52_RS01040 (tssJ) 218606..219082 - 477 WP_001045311.1 type VI secretion system lipoprotein TssJ
T6CP011349 FHA VCV52_RS01045 (tagH) 219085..220572 - 1488 WP_001089706.1 type VI secretion system-associated FHA domain protein TagH
T6CP011008 TssG VCV52_RS01050 (tssG) 220575..221591 - 1017 WP_000510174.1 type VI secretion system baseplate subunit TssG
T6CP011009 TssF VCV52_RS01055 (tssF) 221555..223322 - 1768 pseudogene type VI secretion system baseplate subunit TssF
T6CP011010 TssE VCV52_RS01060 (tssE) 223328..223765 - 438 WP_000221001.1 type VI secretion system baseplate subunit TssE
T6CP011011 TssC VCV52_RS01065 (tssC) 223768..225243 - 1476 WP_000108140.1 type VI secretion system contractile sheath large subunit
T6CP011012 TssB VCV52_RS01070 (tssB) 225287..225793 - 507 WP_000031391.1 type VI secretion system contractile sheath small subunit
VCV52_RS01075 226225..227145 - 921 WP_001901866.1 sel1 repeat family protein
VCV52_RS01080 227213..227497 - 285 WP_000526403.1 type VI secretion system PAAR protein
VCV52_RS01085 228269..229948 + 1680 WP_001275247.1 RluA family pseudouridine synthase
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)
REG0314 experimental TfoY protein Global regulator Vibrio cholerae V52 chromosome (-) - - Unknown (↓)
REG0315 experimental VCV52_RS10780 (TfoX) protein Global regulator Vibrio cholerae V52 chromosome (NZ_KQ410501) 68827..69435 (-) 487807468 Unknown (↓)
REG0316 experimental VCV52_RS01020 (VasH) protein Global regulator Vibrio cholerae V52 chromosome (NZ_KQ410497) 212259..213851 (-) 446006947 Unknown (↓)
REG0317 experimental VCV52_RS13040 (rpoN) protein Global regulator Vibrio cholerae V52 chromosome (NZ_KQ410502) 216932..218395 (-) 446727517 Unknown (↓)
REG0369 experimental VCV52_RS14135 (wigR/phoB/vxrB) protein TCS-RR Vibrio cholerae V52 chromosome (NZ_KQ410503) 201806..202495 (-) 446013262 T6SS transcription level (↓)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
IMU38208 experimental VCV52_RS06820 EFF76630 experimental Vibrio cholerae V52 chromosome (NZ_KQ410498) 431552..432295 (-) WP_000771534.1
IMU00272 experimental VCV52_RS00985 EFF01011 experimental Vibrio cholerae V52 chromosome (NZ_KQ410497) 201656..202024 (-) WP_001061295.1
IMU38206 experimental VCV52_RS20260 EFF76626 experimental Vibrio cholerae V52 chromosome (NZ_KQ410497) 31747..31974 (-) WP_001911595.1
IMU38207 experimental VCV52_RS01465 EFF76627 experimental Vibrio cholerae V52 chromosome (NZ_KQ410497) 315306..316034 (-) WP_000904079.1

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector
ACP00025 experimental VCV52_1391 (tap-1) adaptor Vibrio cholerae V52 chromosome (NZ_KQ410498) 434220..435080 (-) 446336115 VgrG-1 TseL
ACP00026 experimental VCV52_RS01475 (vasW) adaptor Vibrio cholerae V52 chromosome (NZ_KQ410497) 319289..320176 (-) 447180717 - VasX
ACP44067 experimental VCV52_RS01000 (tagA) structure affiliated protein Vibrio cholerae V52 chromosome (NZ_KQ410497) 205313..206575 (-) WP_000073086.1 VCV52_RS01060 (tssE); VCV52_RS01055 (tssF); VCV52_RS01030 (tssL); VCV52_RS01005 (tssM) VCV52_RS06835 (vgrG1); VCV52_RS00990 (vgrG3); VCV52_RS01485 (hcp-2)