Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00790 experimental
T6SS type Type i1
Strain Vibrio cholerae V52
Replicon (RefSeq) chromosome (NZ_KQ410497)
Location 202021..225793
Function anti-bacterial; anti-eukaryotic; effector translocation; virulence; adherence
Comment Cytotoxicity towards Dictyostelium and cultured mammalian macrophages; inflammatory response, diarrhoea and actin cross-linking in mouse intestine; Killing of E. coli, Salmonella and Citrobacter rodentium.
References
[1] Dong TG et al (2013) Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae. Proc Natl Acad Sci U S A . 110(7):2623-8. [PudMed:23362380]
[2] Miyata ST et al (2011) Vibrio cholerae requires the type VI secretion system virulence factor VasX to kill Dictyostelium discoideum. Infect Immun. 79(7):2941-9. [PudMed:21555399]
[3] Unterweger D et al (2014) The Vibrio cholerae type VI secretion system employs diverse effector modules for intraspecific competition. Nat Commun. 2.672916667. [PudMed:24686479]
[4] Ma AT et al (2010) In vivo actin cross-linking induced by Vibrio cholerae type VI secretion system is associated with intestinal inflammation. Proc Natl Acad Sci U S A. 107(9):4365-70. [PudMed:20150509]
[5] Ma AT et al (2009) Translocation of a Vibrio cholerae type VI secretion effector requires bacterial endocytosis by host cells. Cell Host Microbe. 5(3):234-43. [PudMed:19286133]
[6] Shao Y et al (2014) Quorum regulatory small RNAs repress type VI secretion in Vibrio cholerae. Mol Microbiol. 92(5):921-30. [PudMed:24698180]
[7] Stietz MS, Liang X, Li H, Zhang X, Dong TG. et al (2020) TssA-TssM-TagA interaction modulates type VI secretion system sheath-tube assembly in Vibrio cholerae.. Nat Commun. 11(1):5065. [PudMed:33033237]
[8] Pukatzki S et al (2006) Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system. Proc Natl Acad Sci U S A. 103(5):1528-33. [PudMed:16432199]
[9] Borgeaud S et al (2015) Bacterial evolution. The type VI secretion system of Vibrio cholerae fosters horizontal gene transfer. Science. 347(6217):63-7. [PudMed:25554784]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentFHATssATssBTssCTssETssFTssGTssHTssITssJTssKTssLTssMVasH
Number12111111111111


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Component ID
Component
Locus tag (Gene)
Coordinate
Strand
Size (bp)
NCBI ID
Product
VCV52_RS00955 (rbsB)197079..197957-879WP_000737186.1ribose ABC transporter substrate-binding protein RbsB
VCV52_RS00960 (rbsC)198018..198986-969WP_001997313.1ribose ABC transporter permease
VCV52_RS00965 (rbsA)199016..200518-1503WP_000189455.1ribose ABC transporter ATP-binding protein RbsA
VCV52_RS00970 (rbsD)200545..200964-420WP_001911632.1D-ribose pyranase
VCV52_RS00980201248..201508+261WP_000799238.1DUF3012 domain-containing protein
VCV52_RS00985201656..202024-369WP_001061295.1hypothetical protein
T6CP011000TssIVCV52_RS00990 (tssI)202021..205074-3054WP_000113305.1type VI secretion system tip protein VgrG
T6CP011001TssAVCV52_RS01000205313..206575-1263WP_000073086.1ImpA family type VI secretion system protein
T6CP011002TssMVCV52_RS01005 (tssM)206626..210126-3501WP_000225009.1type VI secretion system membrane subunit TssM
T6CP011003TssAVCV52_RS01010 (vasJ)210187..211596-1410WP_000426093.1TssA family type VI secretion system protein VasJ
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flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
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Regulator ID
Locus tag (Gene)
Regulator type
Regulatory pathway
Organism                
Replicon (Accession)
Coordinates (Strand)
NCBI ID
Target (Regulation)
REG0314 experimentalTfoYproteinGlobal regulatorVibrio cholerae V52chromosome (-)--Unknown (↓)
REG0315 experimentalVCV52_RS10780 (TfoX)proteinGlobal regulatorVibrio cholerae V52chromosome (NZ_KQ410501)68827..69435 (-)487807468Unknown (↓)
REG0316 experimentalVCV52_RS01020 (VasH)proteinGlobal regulatorVibrio cholerae V52chromosome (NZ_KQ410497)212259..213851 (-)446006947Unknown (↓)
REG0317 experimentalVCV52_RS13040 (rpoN)proteinGlobal regulatorVibrio cholerae V52chromosome (NZ_KQ410502)216932..218395 (-)446727517Unknown (↓)
REG0369 experimentalVCV52_RS14135 (wigR/phoB/vxrB)proteinTCS-RRVibrio cholerae V52chromosome (NZ_KQ410503)201806..202495 (-)446013262T6SS transcription level (↓)
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experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
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experimental Experimental investigation has been performed.
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Accessory protein ID
Locus tag (Gene)
Type            
Organism                
Replicon (Accession)
Coordinates (Strand)
NCBI ID
Related structure protein
Related effector
ACP00025 experimentalVCV52_1391 (tap-1)adaptorVibrio cholerae V52chromosome (NZ_KQ410498)434220..435080 (-)446336115VgrG-1TseL
ACP00026 experimentalVCV52_RS01475 (vasW)adaptorVibrio cholerae V52chromosome (NZ_KQ410497)319289..320176 (-)447180717-VasX
ACP44067 experimentalVCV52_RS01000 (tagA)structure affiliated proteinVibrio cholerae V52chromosome (NZ_KQ410497)205313..206575 (-)WP_000073086.1VCV52_RS01060 (tssE); VCV52_RS01055 (tssF); VCV52_RS01030 (tssL); VCV52_RS01005 (tssM)VCV52_RS06835 (vgrG1); VCV52_RS00990 (vgrG3); VCV52_RS01485 (hcp-2)
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