Detailed information of T6SS
Note: only experimentally validated proteins are displayed in the graph.
Summary
T6SS ID | T6SS00790 |
T6SS type | Type i1 |
Strain | Vibrio cholerae V52 |
Replicon (RefSeq) | chromosome (NZ_KQ410497) |
Location | 202021..225793 |
Function | anti-bacterial; anti-eukaryotic; effector translocation; virulence; adherence |
Comment | Cytotoxicity towards Dictyostelium and cultured mammalian macrophages; inflammatory response, diarrhoea and actin cross-linking in mouse intestine; Killing of E. coli, Salmonella and Citrobacter rodentium. |
References | [1] Dong TG et al (2013) Identification of T6SS-dependent effector and immunity proteins by Tn-seq in Vibrio cholerae. Proc Natl Acad Sci U S A . 110(7):2623-8. [PudMed:23362380] [2] Miyata ST et al (2011) Vibrio cholerae requires the type VI secretion system virulence factor VasX to kill Dictyostelium discoideum. Infect Immun. 79(7):2941-9. [PudMed:21555399] [3] Unterweger D et al (2014) The Vibrio cholerae type VI secretion system employs diverse effector modules for intraspecific competition. Nat Commun. 2.672916667. [PudMed:24686479] [4] Ma AT et al (2010) In vivo actin cross-linking induced by Vibrio cholerae type VI secretion system is associated with intestinal inflammation. Proc Natl Acad Sci U S A. 107(9):4365-70. [PudMed:20150509] [5] Ma AT et al (2009) Translocation of a Vibrio cholerae type VI secretion effector requires bacterial endocytosis by host cells. Cell Host Microbe. 5(3):234-43. [PudMed:19286133] [6] Shao Y et al (2014) Quorum regulatory small RNAs repress type VI secretion in Vibrio cholerae. Mol Microbiol. 92(5):921-30. [PudMed:24698180] [7] Stietz MS, Liang X, Li H, Zhang X, Dong TG. et al (2020) TssA-TssM-TagA interaction modulates type VI secretion system sheath-tube assembly in Vibrio cholerae.. Nat Commun. 11(1):5065. [PudMed:33033237] [8] Pukatzki S et al (2006) Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system. Proc Natl Acad Sci U S A. 103(5):1528-33. [PudMed:16432199] [9] Borgeaud S et al (2015) Bacterial evolution. The type VI secretion system of Vibrio cholerae fosters horizontal gene transfer. Science. 347(6217):63-7. [PudMed:25554784] |
T6SS components and genome coordinates
Component | FHA | TssA | TssB | TssC | TssE | TssF | TssG | TssH | TssI | TssJ | TssK | TssL | TssM | VasH |
Number | 1 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 |
Component ID | Component | Locus tag (Gene) | Coordinate | Strand | Size (bp) | NCBI ID | Product |
---|---|---|---|---|---|---|---|
VCV52_RS00955 (rbsB) | 197079..197957 | - | 879 | WP_000737186.1 | ribose ABC transporter substrate-binding protein RbsB | ||
VCV52_RS00960 (rbsC) | 198018..198986 | - | 969 | WP_001997313.1 | ribose ABC transporter permease | ||
VCV52_RS00965 (rbsA) | 199016..200518 | - | 1503 | WP_000189455.1 | ribose ABC transporter ATP-binding protein RbsA | ||
VCV52_RS00970 (rbsD) | 200545..200964 | - | 420 | WP_001911632.1 | D-ribose pyranase | ||
VCV52_RS00980 | 201248..201508 | + | 261 | WP_000799238.1 | DUF3012 domain-containing protein | ||
VCV52_RS00985 | 201656..202024 | - | 369 | WP_001061295.1 | hypothetical protein | ||
T6CP011000 | TssI | VCV52_RS00990 (tssI) | 202021..205074 | - | 3054 | WP_000113305.1 | type VI secretion system tip protein VgrG |
T6CP011001 | TssA | VCV52_RS01000 | 205313..206575 | - | 1263 | WP_000073086.1 | ImpA family type VI secretion system protein |
T6CP011002 | TssM | VCV52_RS01005 (tssM) | 206626..210126 | - | 3501 | WP_000225009.1 | type VI secretion system membrane subunit TssM |
T6CP011003 | TssA | VCV52_RS01010 (vasJ) | 210187..211596 | - | 1410 | WP_000426093.1 | TssA family type VI secretion system protein VasJ |
VCV52_RS01015 (tagO) | 211606..212262 | - | 657 | WP_001223375.1 | type VI secretion system-associated protein TagO | ||
T6CP012598 | VasH | VCV52_RS01020 (vasH) | 212259..213851 | - | 1593 | WP_000084802.1 | sigma-54 dependent T6SS transcriptional regulator VasH |
T6CP011004 | TssH | VCV52_RS01025 (tssH) | 213854..216463 | - | 2610 | WP_000619133.1 | type VI secretion system ATPase TssH |
T6CP011005 | TssL | VCV52_RS01030 | 216489..217262 | - | 774 | WP_000083407.1 | type IVB secretion system protein IcmH/DotU |
T6CP011006 | TssK | VCV52_RS01035 (tssK) | 217265..218599 | - | 1335 | WP_000458007.1 | type VI secretion system baseplate subunit TssK |
T6CP011007 | TssJ | VCV52_RS01040 (tssJ) | 218606..219082 | - | 477 | WP_001045311.1 | type VI secretion system lipoprotein TssJ |
T6CP011349 | FHA | VCV52_RS01045 (tagH) | 219085..220572 | - | 1488 | WP_001089706.1 | type VI secretion system-associated FHA domain protein TagH |
T6CP011008 | TssG | VCV52_RS01050 (tssG) | 220575..221591 | - | 1017 | WP_000510174.1 | type VI secretion system baseplate subunit TssG |
T6CP011009 | TssF | VCV52_RS01055 (tssF) | 221555..223322 | - | 1768 | pseudogene | type VI secretion system baseplate subunit TssF |
T6CP011010 | TssE | VCV52_RS01060 (tssE) | 223328..223765 | - | 438 | WP_000221001.1 | type VI secretion system baseplate subunit TssE |
T6CP011011 | TssC | VCV52_RS01065 (tssC) | 223768..225243 | - | 1476 | WP_000108140.1 | type VI secretion system contractile sheath large subunit |
T6CP011012 | TssB | VCV52_RS01070 (tssB) | 225287..225793 | - | 507 | WP_000031391.1 | type VI secretion system contractile sheath small subunit |
VCV52_RS01075 | 226225..227145 | - | 921 | WP_001901866.1 | sel1 repeat family protein | ||
VCV52_RS01080 | 227213..227497 | - | 285 | WP_000526403.1 | type VI secretion system PAAR protein | ||
VCV52_RS01085 | 228269..229948 | + | 1680 | WP_001275247.1 | RluA family pseudouridine synthase |
Download FASTA format files
Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.
Regulator ID | Locus tag (Gene) | Regulator type | Regulatory pathway | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Target (Regulation) |
---|---|---|---|---|---|---|---|---|
REG0314 | TfoY | protein | Global regulator | Vibrio cholerae V52 | chromosome (-) | - | - | Unknown (↓) |
REG0315 | VCV52_RS10780 (TfoX) | protein | Global regulator | Vibrio cholerae V52 | chromosome (NZ_KQ410501) | 68827..69435 (-) | 487807468 | Unknown (↓) |
REG0316 | VCV52_RS01020 (VasH) | protein | Global regulator | Vibrio cholerae V52 | chromosome (NZ_KQ410497) | 212259..213851 (-) | 446006947 | Unknown (↓) |
REG0317 | VCV52_RS13040 (rpoN) | protein | Global regulator | Vibrio cholerae V52 | chromosome (NZ_KQ410502) | 216932..218395 (-) | 446727517 | Unknown (↓) |
REG0369 | VCV52_RS14135 (wigR/phoB/vxrB) | protein | TCS-RR | Vibrio cholerae V52 | chromosome (NZ_KQ410503) | 201806..202495 (-) | 446013262 | T6SS transcription level (↓) |
Experimental investigation has been performed.
Bioinformatics investigation has been performed.
Experimental investigation has been performed.
Bioinformatics investigation has been performed.
Immunity protein ID | Locus tag (Gene) | Cognate effector | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID |
---|---|---|---|---|---|---|
IMU38208 | VCV52_RS06820 | EFF76630 | Vibrio cholerae V52 | chromosome (NZ_KQ410498) | 431552..432295 (-) | WP_000771534.1 |
IMU00272 | VCV52_RS00985 | EFF01011 | Vibrio cholerae V52 | chromosome (NZ_KQ410497) | 201656..202024 (-) | WP_001061295.1 |
IMU38206 | VCV52_RS20260 | EFF76626 | Vibrio cholerae V52 | chromosome (NZ_KQ410497) | 31747..31974 (-) | WP_001911595.1 |
IMU38207 | VCV52_RS01465 | EFF76627 | Vibrio cholerae V52 | chromosome (NZ_KQ410497) | 315306..316034 (-) | WP_000904079.1 |
Experimental investigation has been performed.
Accessory protein ID | Locus tag (Gene) | Type | Organism | Replicon (Accession) | Coordinates (Strand) | NCBI ID | Related structure protein | Related effector |
---|---|---|---|---|---|---|---|---|
ACP00025 | VCV52_1391 (tap-1) | adaptor | Vibrio cholerae V52 | chromosome (NZ_KQ410498) | 434220..435080 (-) | 446336115 | VgrG-1 | TseL |
ACP00026 | VCV52_RS01475 (vasW) | adaptor | Vibrio cholerae V52 | chromosome (NZ_KQ410497) | 319289..320176 (-) | 447180717 | - | VasX |
ACP44067 | VCV52_RS01000 (tagA) | structure affiliated protein | Vibrio cholerae V52 | chromosome (NZ_KQ410497) | 205313..206575 (-) | WP_000073086.1 | VCV52_RS01060 (tssE); VCV52_RS01055 (tssF); VCV52_RS01030 (tssL); VCV52_RS01005 (tssM) | VCV52_RS06835 (vgrG1); VCV52_RS00990 (vgrG3); VCV52_RS01485 (hcp-2) |