SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF01011
Effector type	-
Cognate immunity protein	IMU00272
Related T6SS (Type)	T6SS00790 (Type i1)
Strain	Vibrio cholerae V52
Replicon	chromosome
Sequence	Protein sequence (1018 a.a.); Nucleotide sequence (3054 bp)
Description	-
Reference	[1] Logan SL, Thomas J, Yan J, et al. The Vibrio cholerae type VI secretion system can modulate host intestinal mechanics to displace gut bacterial symbionts. Proc Natl Acad Sci U S A. 2018 Apr 17;115(16):E3779-E3787. doi: 10.1073/pnas.1720133115. Epub 2018 Apr 2. PMID: 29610339 [2] Stietz MS, Liang X, Li H, et al. TssA-TssM-TagA interaction modulates type VI secretion system sheath-tube assembly in Vibrio cholerae. Nat Commun. 2020 Oct 8;11(1):5065. doi: 10.1038/s41467-020-18807-9. PMID: 33033237

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	VCV52_RS00990 (vgrG3)
Coordinates (Strand)	202021..205074 (-)
NCBI ID	WP_000113305.1
RefSeq	NZ_KQ410497
Uniprot ID	UPI0000ED6B39
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	3e-70	41..334
Phage_base_V	PF04717.14	5e-15	391..458
PG_binding_1	PF01471.20	4.4e-08	731..773
DUF2345	PF10106.11	9.7e-05	570..618

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-1017	MARLQFQLKVDGLEDESLVVRGFEGQESLSDSVWRSEPCYGFRYQVDLASALSNLTAEQFVDQTAHLTILRDGQVVQQINGIVRQFSKGDTGHRHTFYSLTLVPALERLSLRSNSRIFQQQSVPEIISILLQEMGIEDYAFALKRECAQREFCVQYRETDLQFLHRIAAEEGLVYSYLHEAQKHTLLFTDSSDSQPKLAKPVPYNALAGGEINLPYVVDLQFKTTAQVSHTELKDYSFKKPAYGFTQRTQGKDIAYQQPNYEHFDAPGRYKDDANGKAFSQIRLEYLRRDALLADAKSDEPLLLAGVRFDLQDHLDLAMNRDWLVVQANHQGTQPQALQEEGGAGATTYSNQLKLIPAHITWRARPCAKPQVDGPMIATVVGPQGEEIYCDNFGRVKVHFPWDRYSSSNEKSSCWVRVAQEWAGSQYGSMAIPRVGHEVIVSFLNGDPDQPIITGRTYHATNTAPYALPDHKTKTVLRTETHQGQGYNELSFEDQAGSEQILLHAQKDWDALIEHDHTEVIRHDQHLTVDNDRFTRIQRNQHLTVEGEVRSKIALDSSHEVGASLQHKVGQRIAVEAGKEISLKSGAKIVVEAGAELTLKAGGSFVKVDAGGVHLVGPAINLNAGGSAGSGSAYGGQLAAAPRMLAQAKPVAELVQPDIAASMQSGAARVIDVASLPTMMPSSANNTANDEPVAEEKTPERILKSDLLKPSDELEKLAKRQASAYRQGNHSDEVKLLQEALIKLGFDLGKAGADGDFGSKTKTAIEQFQKSYQPSHQTHPSYSIGAVDGIVGKGTLLALDEALMDGWVYENNIYQIWPLGKTSEKYESAGRGPGVISTGNGDYGGASYGCYQMSSNLGVVQKYIQSSKFKEFFSGLNPATKEFNVVWQDIASRYPQEFREEQHQFIKRTHYDIQIGHLRGKGLLFEHNRAAVHDLIWSTSVQFGGRTNLIFNALNGQNMESMTDKDIIILVQDYKLVNTERLFKSSPSWWSDLKKRAVSEKKALLELEIDGLEVDIK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9958	-	-

Protein sequence: 1018 a.a. .

                                    >EFF01011 NZ_KQ410497:c205074-202021  (Unknown) [Vibrio cholerae V52]
MARLQFQLKVDGLEDESLVVRGFEGQESLSDSVWRSEPCYGFRYQVDLASALSNLTAEQFVDQTAHLTILRDGQVVQQIN
GIVRQFSKGDTGHRHTFYSLTLVPALERLSLRSNSRIFQQQSVPEIISILLQEMGIEDYAFALKRECAQREFCVQYRETD
LQFLHRIAAEEGLVYSYLHEAQKHTLLFTDSSDSQPKLAKPVPYNALAGGEINLPYVVDLQFKTTAQVSHTELKDYSFKK
PAYGFTQRTQGKDIAYQQPNYEHFDAPGRYKDDANGKAFSQIRLEYLRRDALLADAKSDEPLLLAGVRFDLQDHLDLAMN
RDWLVVQANHQGTQPQALQEEGGAGATTYSNQLKLIPAHITWRARPCAKPQVDGPMIATVVGPQGEEIYCDNFGRVKVHF
PWDRYSSSNEKSSCWVRVAQEWAGSQYGSMAIPRVGHEVIVSFLNGDPDQPIITGRTYHATNTAPYALPDHKTKTVLRTE
THQGQGYNELSFEDQAGSEQILLHAQKDWDALIEHDHTEVIRHDQHLTVDNDRFTRIQRNQHLTVEGEVRSKIALDSSHE
VGASLQHKVGQRIAVEAGKEISLKSGAKIVVEAGAELTLKAGGSFVKVDAGGVHLVGPAINLNAGGSAGSGSAYGGQLAA
APRMLAQAKPVAELVQPDIAASMQSGAARVIDVASLPTMMPSSANNTANDEPVAEEKTPERILKSDLLKPSDELEKLAKR
QASAYRQGNHSDEVKLLQEALIKLGFDLGKAGADGDFGSKTKTAIEQFQKSYQPSHQTHPSYSIGAVDGIVGKGTLLALD
EALMDGWVYENNIYQIWPLGKTSEKYESAGRGPGVISTGNGDYGGASYGCYQMSSNLGVVQKYIQSSKFKEFFSGLNPAT
KEFNVVWQDIASRYPQEFREEQHQFIKRTHYDIQIGHLRGKGLLFEHNRAAVHDLIWSTSVQFGGRTNLIFNALNGQNME
SMTDKDIIILVQDYKLVNTERLFKSSPSWWSDLKKRAVSEKKALLELEIDGLEVDIK*

                                

Nucleotide sequence: 3054 bp .

                                    >EFF01011 NZ_KQ410497:c205074-202021  (Unknown) [Vibrio cholerae V52]
ATGGCAAGGTTACAGTTTCAATTAAAGGTGGATGGCCTTGAGGATGAATCCCTCGTCGTACGCGGATTTGAAGGGCAAGA
GTCCTTGTCTGATTCTGTATGGCGCAGTGAACCCTGCTATGGTTTTCGCTATCAGGTTGATTTAGCCAGTGCGCTAAGTA
ATCTCACGGCTGAGCAATTTGTAGACCAAACCGCACATTTAACCATTCTGCGTGACGGGCAAGTGGTGCAGCAAATAAAC
GGTATTGTGCGTCAATTCAGCAAAGGGGATACCGGCCATCGACATACTTTCTATTCATTAACGCTAGTTCCTGCGTTGGA
AAGGCTTTCTTTACGCAGTAATAGCCGTATTTTCCAGCAGCAAAGCGTGCCAGAGATTATCTCTATTTTACTGCAAGAGA
TGGGGATTGAAGATTACGCCTTTGCCCTTAAGCGTGAGTGTGCCCAGCGAGAGTTTTGTGTACAGTATCGTGAAACAGAT
TTGCAGTTTTTACACCGTATCGCCGCCGAAGAAGGTTTGGTTTACAGCTATCTACATGAAGCGCAGAAACACACCTTGCT
GTTTACCGATAGCTCGGACAGCCAACCGAAGTTAGCCAAGCCAGTACCTTATAATGCGCTGGCGGGTGGCGAGATCAATC
TCCCTTATGTGGTCGATCTGCAATTCAAGACCACCGCGCAAGTGAGCCATACCGAGCTAAAGGATTACAGCTTTAAAAAG
CCAGCTTACGGATTTACTCAGCGCACGCAGGGTAAAGATATCGCTTATCAGCAGCCAAATTATGAGCATTTTGATGCTCC
GGGACGCTACAAAGACGATGCAAATGGCAAGGCCTTTAGCCAAATTCGCTTAGAGTATTTACGCCGCGATGCTTTGCTTG
CTGATGCGAAGAGTGACGAACCTTTGCTGTTGGCGGGAGTGCGTTTTGACTTGCAAGATCACCTTGATCTCGCAATGAAT
CGAGATTGGCTCGTGGTACAAGCCAATCACCAAGGGACGCAGCCACAAGCGTTACAAGAAGAGGGGGGGGCAGGCGCTAC
CACTTACAGCAATCAGCTAAAACTTATCCCCGCTCACATCACTTGGCGAGCAAGGCCTTGTGCTAAGCCGCAAGTGGATG
GTCCCATGATCGCTACCGTAGTTGGGCCACAAGGTGAAGAGATTTATTGTGATAACTTTGGTCGCGTGAAAGTGCATTTC
CCGTGGGATCGCTACTCAAGTAGCAATGAGAAAAGCTCTTGCTGGGTACGAGTGGCACAAGAATGGGCGGGTAGCCAATA
CGGTAGTATGGCGATTCCGCGAGTCGGCCATGAAGTGATTGTTTCGTTCCTGAATGGCGATCCGGATCAACCCATCATCA
CGGGTCGTACGTACCATGCGACCAATACCGCGCCTTACGCCTTGCCTGACCACAAAACCAAAACCGTACTGCGAACTGAG
ACTCACCAAGGGCAAGGCTACAACGAACTGAGTTTTGAGGATCAAGCGGGCAGCGAACAGATTTTGCTGCATGCACAAAA
AGATTGGGATGCGTTGATTGAGCATGATCACACCGAAGTGATTCGCCACGATCAGCATCTCACTGTGGACAATGATCGCT
TTACCCGCATTCAGCGCAACCAACATTTAACGGTTGAAGGTGAAGTCCGTAGCAAAATCGCACTCGATAGCAGCCATGAA
GTCGGCGCGTCACTACAACACAAAGTAGGGCAACGCATTGCTGTCGAGGCGGGCAAAGAGATTTCACTCAAAAGCGGCGC
AAAAATCGTGGTTGAAGCCGGAGCGGAGTTAACCCTAAAAGCAGGGGGCAGCTTTGTAAAAGTCGATGCAGGTGGCGTGC
ACTTAGTCGGTCCTGCCATTAACCTAAACGCGGGCGGCAGTGCAGGCAGCGGTAGCGCTTATGGTGGGCAATTAGCCGCA
GCACCAAGAATGTTAGCGCAAGCTAAACCAGTAGCTGAATTGGTTCAGCCGGATATTGCTGCCTCAATGCAATCAGGCGC
GGCGCGTGTTATTGATGTGGCATCACTCCCCACAATGATGCCGAGTTCAGCCAATAACACGGCAAATGATGAGCCTGTGG
CCGAAGAGAAAACACCGGAGCGAATTTTAAAATCCGATCTCCTCAAACCATCCGATGAGTTAGAGAAACTCGCCAAGCGA
CAAGCGAGTGCTTATCGTCAAGGTAATCACAGCGATGAGGTTAAGTTACTACAAGAAGCGCTCATAAAATTGGGGTTTGA
TCTCGGGAAAGCAGGAGCTGATGGTGACTTTGGGAGCAAAACTAAAACCGCGATTGAGCAGTTCCAGAAAAGCTATCAAC
CAAGCCACCAAACCCACCCGTCTTACAGCATTGGCGCTGTTGACGGTATTGTCGGCAAAGGTACACTACTGGCACTAGAC
GAAGCCTTGATGGATGGGTGGGTGTATGAGAATAATATTTATCAAATATGGCCTCTAGGTAAAACGTCAGAGAAATATGA
GTCGGCAGGAAGAGGCCCAGGGGTTATATCAACAGGAAATGGAGATTATGGTGGGGCTTCATATGGTTGCTATCAAATGT
CATCAAATCTTGGTGTGGTACAAAAATATATTCAGTCATCAAAATTCAAAGAATTCTTTAGTGGATTAAATCCCGCTACG
AAAGAGTTTAATGTTGTTTGGCAGGATATAGCTTCAAGATATCCTCAGGAATTCAGAGAAGAACAACACCAATTTATTAA
GAGAACTCATTATGATATACAAATAGGACATCTTAGAGGGAAAGGACTTTTGTTTGAACATAATCGAGCTGCTGTACATG
ATTTGATCTGGTCTACTTCAGTACAGTTTGGTGGGAGAACTAATTTGATTTTCAATGCATTGAATGGACAAAATATGGAA
AGTATGACTGATAAGGACATCATTATTCTGGTACAAGATTACAAGCTTGTTAATACAGAAAGGCTTTTTAAGTCTTCGCC
ATCATGGTGGAGTGATTTAAAAAAACGTGCGGTATCAGAGAAAAAAGCTTTACTTGAACTAGAAATTGACGGTTTGGAGG
TTGATATAAAATGA

                                