Detailed information of accessory protein
Summary
| Accessory protein ID | ACP00025  |
| Type | adaptor |
| Related T6SS (Type) | T6SS00790 (Type i1) |
| Strain | Vibrio cholerae V52 |
| Replicon | chromosome |
| Related structure protein | VgrG-1 |
| Related effector | TseL |
| Description | Tap-1, DUF4123 domain-containing protein. |
| Reference | [1] Unterweger D, Kostiuk B, Ötjengerdes R, et al. Chimeric adaptor proteins translocate diverse type VI secretion system effectors in Vibrio cholerae. EMBO J. 2015 Aug 13;34(16):2198-210. doi: 10.15252/embj.201591163. Epub 2015 Jul 19. PMID: 26194724 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | VCV52_1391 (tap-1) |
| Coordinates (Strand) | 434220..435080 (-) |
| NCBI ID | 446336115 |
| RefSeq | NZ_KQ410498 |
| Uniprot ID | UPI00005F3639 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| DUF4123 | PF13503.8 | 8.3e-16 | 28..140 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-286 | MATHYCLVENGMEKMLLQWLSEQTSNVYWLADHLTFKQAVANNSGIVFDGTQVVFHGETFAPVMALSPWLVPVSDMVSDIDYECLQQGIFLSCSCPSTELLSHLQSLLIAALEGEEVLFRFYDRQVIVPMLERMDEIEKNQFLGKINQLVVFDSHEKNRLVSFTNTSDAQFTAKKTTWWVIKRHHLEAHENLPLVQANLESWLWRHFPKVMNEYLIQGRDIASMLAPSLSVPEQTLTYRVLSAAIVAVVGAEQLTQPSMIEFLRDYNNEEAQLALHALTRQFELRG |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9975 | - | - |
Protein sequence: 287 a.a.
>ACP00025 NZ_KQ410498:c435080-434220 [Vibrio cholerae V52]
MATHYCLVENGMEKMLLQWLSEQTSNVYWLADHLTFKQAVANNSGIVFDGTQVVFHGETFAPVMALSPWLVPVSDMVSDI
DYECLQQGIFLSCSCPSTELLSHLQSLLIAALEGEEVLFRFYDRQVIVPMLERMDEIEKNQFLGKINQLVVFDSHEKNRL
VSFTNTSDAQFTAKKTTWWVIKRHHLEAHENLPLVQANLESWLWRHFPKVMNEYLIQGRDIASMLAPSLSVPEQTLTYRV
LSAAIVAVVGAEQLTQPSMIEFLRDYNNEEAQLALHALTRQFELRG*
MATHYCLVENGMEKMLLQWLSEQTSNVYWLADHLTFKQAVANNSGIVFDGTQVVFHGETFAPVMALSPWLVPVSDMVSDI
DYECLQQGIFLSCSCPSTELLSHLQSLLIAALEGEEVLFRFYDRQVIVPMLERMDEIEKNQFLGKINQLVVFDSHEKNRL
VSFTNTSDAQFTAKKTTWWVIKRHHLEAHENLPLVQANLESWLWRHFPKVMNEYLIQGRDIASMLAPSLSVPEQTLTYRV
LSAAIVAVVGAEQLTQPSMIEFLRDYNNEEAQLALHALTRQFELRG*
Nucleotide sequence: 861 bp
>ACP00025 NZ_KQ410498:c435080-434220 [Vibrio cholerae V52]
ATGGCAACGCATTATTGCTTAGTGGAAAATGGAATGGAAAAGATGCTTTTACAATGGTTGAGTGAACAGACATCAAACGT
CTATTGGCTTGCTGATCATTTAACGTTTAAACAGGCGGTGGCGAATAACAGTGGAATTGTATTTGATGGTACACAAGTTG
TTTTCCACGGAGAGACCTTTGCCCCTGTGATGGCGTTATCGCCCTGGTTAGTCCCTGTCTCTGATATGGTATCGGATATC
GACTACGAGTGCTTACAACAAGGTATATTCCTAAGTTGCTCCTGTCCCTCCACAGAGTTATTAAGCCATCTCCAAAGCCT
TTTGATTGCAGCACTGGAAGGCGAAGAAGTACTGTTTCGTTTTTATGATAGGCAGGTCATTGTGCCTATGCTTGAACGAA
TGGATGAAATAGAAAAAAATCAGTTCTTAGGTAAGATAAATCAGTTAGTCGTGTTCGATAGCCATGAGAAAAATCGGTTA
GTCTCATTTACCAATACATCAGACGCTCAGTTTACGGCCAAAAAGACAACGTGGTGGGTGATCAAACGACACCATTTGGA
GGCTCATGAGAATTTGCCTCTTGTTCAAGCTAACCTCGAATCTTGGCTATGGCGGCATTTTCCAAAAGTGATGAACGAGT
ACTTAATTCAAGGGCGTGATATCGCTTCCATGCTCGCTCCATCTCTCTCTGTTCCTGAACAAACCTTAACATATCGTGTG
TTAAGTGCCGCTATCGTTGCTGTTGTCGGTGCCGAACAATTGACTCAGCCTAGCATGATTGAATTTCTACGAGACTATAA
CAACGAAGAAGCTCAGCTCGCTCTGCATGCTTTAACTCGTCAATTTGAACTAAGGGGATAA
ATGGCAACGCATTATTGCTTAGTGGAAAATGGAATGGAAAAGATGCTTTTACAATGGTTGAGTGAACAGACATCAAACGT
CTATTGGCTTGCTGATCATTTAACGTTTAAACAGGCGGTGGCGAATAACAGTGGAATTGTATTTGATGGTACACAAGTTG
TTTTCCACGGAGAGACCTTTGCCCCTGTGATGGCGTTATCGCCCTGGTTAGTCCCTGTCTCTGATATGGTATCGGATATC
GACTACGAGTGCTTACAACAAGGTATATTCCTAAGTTGCTCCTGTCCCTCCACAGAGTTATTAAGCCATCTCCAAAGCCT
TTTGATTGCAGCACTGGAAGGCGAAGAAGTACTGTTTCGTTTTTATGATAGGCAGGTCATTGTGCCTATGCTTGAACGAA
TGGATGAAATAGAAAAAAATCAGTTCTTAGGTAAGATAAATCAGTTAGTCGTGTTCGATAGCCATGAGAAAAATCGGTTA
GTCTCATTTACCAATACATCAGACGCTCAGTTTACGGCCAAAAAGACAACGTGGTGGGTGATCAAACGACACCATTTGGA
GGCTCATGAGAATTTGCCTCTTGTTCAAGCTAACCTCGAATCTTGGCTATGGCGGCATTTTCCAAAAGTGATGAACGAGT
ACTTAATTCAAGGGCGTGATATCGCTTCCATGCTCGCTCCATCTCTCTCTGTTCCTGAACAAACCTTAACATATCGTGTG
TTAAGTGCCGCTATCGTTGCTGTTGTCGGTGCCGAACAATTGACTCAGCCTAGCATGATTGAATTTCTACGAGACTATAA
CAACGAAGAAGCTCAGCTCGCTCTGCATGCTTTAACTCGTCAATTTGAACTAAGGGGATAA