SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF76631
Effector type	-
Cognate immunity protein	-
Related T6SS (Type)	T6SS00790 (Type i1)
Strain	Vibrio cholerae V52
Replicon	chromosome
Sequence	Protein sequence (1164 a.a.); Nucleotide sequence (3492 bp)
Description	-
Reference	[1] Logan SL, Thomas J, Yan J, et al. The Vibrio cholerae type VI secretion system can modulate host intestinal mechanics to displace gut bacterial symbionts. Proc Natl Acad Sci U S A. 2018 Apr 17;115(16):E3779-E3787. doi: 10.1073/pnas.1720133115. Epub 2018 Apr 2. PMID: 29610339

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	VCV52_RS06835 (vgrG1)
Coordinates (Strand)	435059..438550 (-)
NCBI ID	WP_000212116.1
RefSeq	NZ_KQ410498
Uniprot ID	VGRG1_VIBCH, A0A7U8ZS88_VIBCL, A0A0H3Q601_VIBCL, C3LMB1_VIBCM
KEGG ID	vch:VC1416, vcm:VCM66_1371
PDB ID	4GQK

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ACD	PF16671.7	1.6e-238	726..1111
Phage_GPD	PF05954.13	3.9e-77	41..334
Phage_base_V	PF04717.14	1.1e-13	392..458

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-1163	MATLAYSIEVEGLEDETLVVRGFHGQESLSNSVFLGQACYGFRYEVQLASRVSNLTAEQMVDKRAELKLYRNSQLVQRVHGIVRAFSQGDIGHHHTFYQLTLVPALERLSLRHNSRIFQKQTVPEILSILLQEMGINDYAFALKRDGVQREFCVQYRESDIDFLHRLAAEEGLVYSFVHEAGKHTLYFSDASDSLSKLPEPIPYNALVGGAIDTPYIHGLTYRTQAEVSEVQLKDYSFKKPAYSFLQTVQGTELDYQQTRYQHFDAPGRYKDDVNGAAFSQIRLDYLRRHAHTATGQSNEPLLRAGYKFDLQEHLDPAMNRDWVVVSINHQGEQPQALQEDGGSGATTYSNQFSLIPGHLHWRAEPQPKPQVDGPMIATVVGPEGEEIFCDEHGRVKIHFPWDRYSNGNEQSSCWVRVSQGWAGSQYGFIAIPRIGHEVIVEFLNGDPDQPIITGRTYHATNTPPYTLPEHKTKTVLRTETHQGEGFNELSFEDQAGKEQIYLHAQKDFDGLIENDHTTVIRHDHHLTVENDQFTQIKHNQHLTVEWESREAVTGEQVLSIEGSLHVKTGKVWVNEAGTEIHVKAGQKVVIEAGSEITVKAGGSFVKVDPAGVHLSGALVNLNSGGSAGSGSGFGGAMPALPGGLEPAVALAPPQTISYQALLQAEQANVPAVKVCPLAAQEATPAVNSITPPPPPPIAPPMAPPQPIMNPQPTANAQPNLGRSTKATPDFPTHFPKSSIGIENELAGLVVAMPANSAQKFGYVKSAQGDALFMLTKDMNQGSYQRPPSLQDGKNYQNWQTHTVELVSYPCEMDDKAAVETRKQAMLWLATHFTTHIDQSNHQPLAPIQSEDGRFVIEITNAKHVIAAGNGISAESQGQTITMTPSGQQATVGVAAKGFGTSATPELRLLESAPWYQKSLKSQFASLTSAENLDDKELAANVFAYLTSIYLKTAELAKKFGIYINEWDPMSEQITPNANGLTDPKVKNAWEILPRTKPSKIVEILSKSDAKAVMKHIKPQLQSRYSESLSKNVFQYFQDGGEVAGHGINNATVGDKHSPELAILFEFRTVPNELQSYLPKTESTTKSEVKLLDQFDPMKRKTVIQQVESLVQNSGDAFDKWYQSYRDSMNQPPVKNAKKIASANQKAQWVKEHNPQEWQRIIA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9832	-	-

Protein sequence: 1164 a.a. .

                                    >EFF76631 NZ_KQ410498:c438550-435059  (Unknown) [Vibrio cholerae V52]
MATLAYSIEVEGLEDETLVVRGFHGQESLSNSVFLGQACYGFRYEVQLASRVSNLTAEQMVDKRAELKLYRNSQLVQRVH
GIVRAFSQGDIGHHHTFYQLTLVPALERLSLRHNSRIFQKQTVPEILSILLQEMGINDYAFALKRDGVQREFCVQYRESD
IDFLHRLAAEEGLVYSFVHEAGKHTLYFSDASDSLSKLPEPIPYNALVGGAIDTPYIHGLTYRTQAEVSEVQLKDYSFKK
PAYSFLQTVQGTELDYQQTRYQHFDAPGRYKDDVNGAAFSQIRLDYLRRHAHTATGQSNEPLLRAGYKFDLQEHLDPAMN
RDWVVVSINHQGEQPQALQEDGGSGATTYSNQFSLIPGHLHWRAEPQPKPQVDGPMIATVVGPEGEEIFCDEHGRVKIHF
PWDRYSNGNEQSSCWVRVSQGWAGSQYGFIAIPRIGHEVIVEFLNGDPDQPIITGRTYHATNTPPYTLPEHKTKTVLRTE
THQGEGFNELSFEDQAGKEQIYLHAQKDFDGLIENDHTTVIRHDHHLTVENDQFTQIKHNQHLTVEWESREAVTGEQVLS
IEGSLHVKTGKVWVNEAGTEIHVKAGQKVVIEAGSEITVKAGGSFVKVDPAGVHLSGALVNLNSGGSAGSGSGFGGAMPA
LPGGLEPAVALAPPQTISYQALLQAEQANVPAVKVCPLAAQEATPAVNSITPPPPPPIAPPMAPPQPIMNPQPTANAQPN
LGRSTKATPDFPTHFPKSSIGIENELAGLVVAMPANSAQKFGYVKSAQGDALFMLTKDMNQGSYQRPPSLQDGKNYQNWQ
THTVELVSYPCEMDDKAAVETRKQAMLWLATHFTTHIDQSNHQPLAPIQSEDGRFVIEITNAKHVIAAGNGISAESQGQT
ITMTPSGQQATVGVAAKGFGTSATPELRLLESAPWYQKSLKSQFASLTSAENLDDKELAANVFAYLTSIYLKTAELAKKF
GIYINEWDPMSEQITPNANGLTDPKVKNAWEILPRTKPSKIVEILSKSDAKAVMKHIKPQLQSRYSESLSKNVFQYFQDG
GEVAGHGINNATVGDKHSPELAILFEFRTVPNELQSYLPKTESTTKSEVKLLDQFDPMKRKTVIQQVESLVQNSGDAFDK
WYQSYRDSMNQPPVKNAKKIASANQKAQWVKEHNPQEWQRIIA*

                                

Nucleotide sequence: 3492 bp .

                                    >EFF76631 NZ_KQ410498:c438550-435059  (Unknown) [Vibrio cholerae V52]
ATGGCGACATTAGCGTACAGCATTGAGGTGGAAGGGCTGGAAGATGAGACTCTGGTGGTCAGAGGCTTCCACGGGCAGGA
GTCACTCTCTAATAGTGTTTTTTTAGGGCAAGCCTGTTACGGGTTTCGCTACGAAGTGCAACTGGCGAGTCGGGTATCAA
ACCTCACCGCCGAGCAGATGGTGGATAAGCGTGCCGAGCTCAAGCTTTACCGCAACTCACAGTTGGTGCAGCGCGTGCAC
GGCATTGTGCGTGCTTTCAGCCAAGGCGATATCGGCCATCACCACACCTTCTACCAACTCACTTTAGTCCCTGCCTTAGA
GCGCTTATCCCTGCGTCATAACAGCCGCATTTTCCAAAAGCAGACTGTCCCTGAGATTCTCTCTATCTTGCTGCAAGAGA
TGGGCATCAACGATTACGCTTTTGCGCTCAAACGTGATGGCGTGCAGCGTGAGTTTTGTGTGCAGTACCGCGAAAGCGAT
ATCGACTTTCTGCACCGCCTCGCCGCTGAAGAAGGTTTGGTGTACAGCTTTGTCCATGAAGCGGGCAAACATACCCTCTA
TTTCAGTGATGCCAGTGATAGCCTAAGCAAACTGCCGGAGCCTATCCCATACAACGCTTTAGTCGGTGGCGCGATAGATA
CCCCGTATATCCACGGCTTAACCTATCGCACCCAAGCAGAAGTGAGCGAAGTTCAGCTCAAAGACTACAGCTTCAAAAAG
CCAGCCTACTCCTTTTTGCAAACCGTGCAGGGTACTGAGCTGGATTATCAGCAAACGCGCTACCAACATTTTGATGCGCC
GGGGCGCTACAAAGACGATGTGAACGGCGCCGCCTTTAGCCAAATCCGTTTAGACTACCTGCGCCGCCATGCCCATACCG
CAACAGGGCAGAGCAACGAGCCGCTGCTGCGTGCCGGCTATAAGTTTGACCTGCAAGAGCACCTTGACCCCGCGATGAAC
CGCGATTGGGTGGTAGTGTCGATTAACCACCAAGGTGAGCAGCCGCAAGCGCTACAAGAAGACGGTGGCAGCGGCGCTAC
TACTTACAGCAATCAGTTCAGCTTAATTCCGGGGCATCTTCACTGGCGCGCCGAGCCACAACCCAAACCGCAAGTCGATG
GTCCAATGATTGCCACGGTCGTCGGTCCTGAAGGCGAAGAAATCTTCTGTGATGAACATGGGCGGGTGAAAATCCATTTC
CCGTGGGACCGTTACTCGAACGGTAACGAGCAAAGCTCCTGTTGGGTACGGGTGTCGCAAGGTTGGGCGGGCAGTCAGTA
TGGCTTTATCGCTATTCCGCGCATCGGCCATGAAGTGATTGTAGAGTTTTTAAACGGTGACCCAGACCAACCGATTATCA
CTGGCCGCACTTATCATGCGACCAACACGCCGCCATACACCTTGCCTGAGCACAAAACCAAAACCGTGCTGCGCACCGAG
ACGCACCAAGGGGAGGGCTTTAACGAGCTCAGTTTTGAAGACCAAGCGGGCAAAGAGCAAATCTACCTGCATGCGCAGAA
AGATTTTGATGGTTTGATTGAAAACGACCACACCACGGTCATTCGTCACGACCATCACCTGACCGTTGAAAACGACCAAT
TTACCCAGATTAAGCACAACCAACACCTGACGGTGGAGTGGGAAAGTCGAGAAGCGGTAACGGGCGAGCAAGTGCTGAGT
ATTGAAGGCTCGCTGCATGTGAAAACAGGCAAAGTTTGGGTCAATGAAGCGGGTACAGAGATCCATGTAAAAGCGGGACA
GAAAGTGGTGATTGAAGCGGGCTCCGAGATTACCGTTAAAGCGGGCGGCAGCTTTGTGAAAGTCGATCCGGCTGGGGTGC
ATCTGTCCGGTGCTTTGGTCAATCTCAACTCTGGCGGCAGCGCTGGTAGTGGTAGCGGCTTTGGCGGTGCAATGCCCGCC
TTACCGGGCGGCTTAGAACCCGCCGTGGCACTGGCTCCACCGCAAACCATCTCTTACCAAGCGTTGCTACAGGCCGAGCA
AGCGAATGTTCCTGCGGTGAAGGTGTGTCCTTTAGCCGCTCAAGAAGCAACGCCTGCGGTGAATAGTATCACGCCGCCTC
CACCACCACCGATTGCTCCGCCAATGGCCCCACCACAACCAATAATGAATCCACAGCCAACCGCTAACGCGCAGCCTAAT
CTTGGAAGATCCACTAAGGCAACGCCGGATTTTCCAACACATTTCCCGAAGTCATCCATTGGCATTGAGAATGAGTTAGC
TGGTCTAGTGGTGGCTATGCCAGCCAACTCGGCACAGAAATTTGGCTACGTGAAAAGTGCACAAGGTGATGCGCTGTTTA
TGTTAACGAAAGACATGAACCAAGGTTCGTATCAGCGGCCACCTTCATTACAAGATGGAAAAAATTATCAGAATTGGCAG
ACGCATACAGTAGAGTTGGTGAGCTACCCTTGTGAAATGGACGATAAAGCTGCCGTTGAAACACGCAAACAAGCCATGTT
GTGGTTAGCAACCCACTTTACTACGCATATCGACCAGTCCAATCATCAACCATTAGCTCCTATTCAAAGTGAAGACGGAC
GCTTTGTTATCGAAATCACTAATGCGAAGCATGTGATTGCTGCGGGCAATGGGATTTCTGCAGAATCTCAAGGTCAAACA
ATCACGATGACACCAAGTGGACAACAAGCCACAGTGGGTGTTGCTGCTAAAGGTTTCGGGACGAGTGCAACGCCAGAATT
AAGGTTACTTGAATCGGCACCTTGGTATCAGAAATCACTCAAGAGCCAATTTGCATCTCTAACGAGCGCAGAAAACTTGG
ATGATAAAGAGTTAGCTGCAAACGTATTTGCTTATCTGACGTCTATTTATTTAAAAACAGCAGAGCTAGCGAAGAAGTTT
GGTATTTACATCAATGAATGGGACCCCATGTCGGAGCAAATTACTCCAAATGCGAATGGATTAACGGATCCAAAAGTAAA
AAATGCATGGGAAATATTGCCACGTACAAAACCAAGCAAAATAGTAGAAATACTCAGTAAGAGTGATGCCAAAGCGGTTA
TGAAACATATCAAGCCTCAATTGCAGTCTCGCTATTCTGAGAGTTTGTCGAAAAATGTATTTCAATATTTCCAAGATGGT
GGTGAAGTAGCCGGACACGGTATTAATAATGCGACGGTCGGTGATAAACATAGTCCGGAATTGGCGATTTTGTTTGAGTT
CAGAACTGTTCCGAATGAACTGCAAAGTTATTTGCCTAAAACAGAATCGACAACTAAGTCAGAAGTGAAGTTACTCGATC
AGTTTGATCCTATGAAGCGTAAAACAGTAATTCAACAAGTAGAATCGCTTGTGCAAAATAGTGGCGATGCATTTGATAAA
TGGTACCAAAGCTACCGAGACTCCATGAATCAACCTCCCGTTAAAAATGCGAAGAAAATTGCTTCCGCGAATCAAAAAGC
ACAATGGGTTAAAGAGCATAATCCTCAAGAATGGCAACGCATTATTGCTTAG

                                