Detailed information of regulator


Summary

Regulator ID REG0316 experimental
Regulator type protein
Regulatory pathway (from literatures) Global regulator
KEGG pathway (determined by BlastKOALA) -
Related T6SS T6SS00790 experimental (Type i1)
Strain Vibrio cholerae V52
Replicon chromosome
Sequence Protein sequence (531 a.a.); Nucleotide sequence (1593 bp)
Description VasH is essential for T6SS-mediated secretion of Hcp and VasX and bacterial killing by V52; Whole-genome microarray-based transcriptome analysis showed that transcriptional expression of hcp-1 (VC1415) and hcp-2 (VCA0017), was significantly reduced in the vasH deletion mutant SP117 compared with wild-type V52
Reference
[1] Kitaoka M, Miyata ST, Brooks TM, et al. VasH is a transcriptional regulator of the type VI secretion system functional in endemic and pandemic Vibrio cholerae. J Bacteriol. 2011 Dec;193(23):6471-82. doi: 10.1128/JB.05414-11. Epub 2011 Sep 23. PMID: 21949076
[2] Dong TG, Mekalanos JJ. Characterization of the RpoN regulon reveals differential regulation of T6SS and new flagellar operons in Vibrio cholerae O37 strain V52. Nucleic Acids Res. 2012 Sep;40(16):7766-75. doi: 10.1093/nar/gks567. Epub 2012 Jun 20. PMID: 22723378
[3] Pukatzki S, Ma AT, Sturtevant D, et al. Identification of a conserved bacterial protein secretion system in Vibrio cholerae using the Dictyostelium host model system. Proc Natl Acad Sci U S A. 2006 Jan 31;103(5):1528-33. Epub 2006 Jan 23. PMID: 16432199
[4] Manera K, Caro F, Li H, et al. Sensing of intracellular Hcp levels controls T6SS expression in Vibrio cholerae. Proc Natl Acad Sci U S A. 2021 Jun 22;118(25). pii: e2104813118. doi: 10.1073/pnas.2104813118. PMID: 34161288
External database links

Locus tag (Gene) VCV52_RS01020 (VasH)
Coordinates (Strand) 212259..213851 (-)
NCBI ID 446006947
RefSeq NZ_KQ410497
Uniprot ID UPI00005F4E9C
KEGG ID -
KEGG pathway (determined by BlastKOALA) -
PDB ID -
  Target
Target Regulation (↓/↑) Target sequence
Unknown ↓ -
Pfam domain hit(s) of regulator

Domain Pfam ID E-value Aligned region
Sigma54_activat PF00158.28 1.5e-68 193..359
HTH_8 PF02954.21 1.8e-06 486..521
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-530 MSQWLAFATQLVGVRKSHQLALQFVDLLTQGLDLSDSLLLLPSSDGRLLVPHDPQRQFAWSVTDFDVPFAHVLQSSNAMHLTAEELVFWQSNRTFSQLTSRVGMFDSVWIQPLPMHTRQVHSILLLMGESHGIVSAFENADFLKFIEVFSQQWSLLNDMEREEQRRLELKQSLTDIERDSAQRSLANALSRTLIGESAAMQKLREQIVSAANSQLSVMVQGETGTGKELVAAAVHELSSRKSAPFVAINCAAIPEHLLESELFGYCKGAFSGADSDKQGLIAQANGGTLFLDEIGDMPLTLQAKLLRVLESRTFRPLGGKQELSSDFRLVSATHVNLLDQVRKKEFRQDLYYRLFQYPITLPRLAARLEDIELLSEHFVRVFNLQHNTRIRGLNYRAIDCLKQYDFPGNVRELKHLIEFGCAQTADGTQVEASCFAHRLQTLPCLAPETTPVAVSVETENVDLEPSVALAGEPNFAVIHDLKQAVSQFEALIISERLNRFAGDRAKAAKSLGIPKRTLAYKCLKLEIKTP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9730 - -


  Protein sequence of regulator: 531 a.a.    .

>REG0316 NZ_KQ410497:c213851-212259 [Vibrio cholerae V52]
MSQWLAFATQLVGVRKSHQLALQFVDLLTQGLDLSDSLLLLPSSDGRLLVPHDPQRQFAWSVTDFDVPFAHVLQSSNAMH
LTAEELVFWQSNRTFSQLTSRVGMFDSVWIQPLPMHTRQVHSILLLMGESHGIVSAFENADFLKFIEVFSQQWSLLNDME
REEQRRLELKQSLTDIERDSAQRSLANALSRTLIGESAAMQKLREQIVSAANSQLSVMVQGETGTGKELVAAAVHELSSR
KSAPFVAINCAAIPEHLLESELFGYCKGAFSGADSDKQGLIAQANGGTLFLDEIGDMPLTLQAKLLRVLESRTFRPLGGK
QELSSDFRLVSATHVNLLDQVRKKEFRQDLYYRLFQYPITLPRLAARLEDIELLSEHFVRVFNLQHNTRIRGLNYRAIDC
LKQYDFPGNVRELKHLIEFGCAQTADGTQVEASCFAHRLQTLPCLAPETTPVAVSVETENVDLEPSVALAGEPNFAVIHD
LKQAVSQFEALIISERLNRFAGDRAKAAKSLGIPKRTLAYKCLKLEIKTP*

  Nucleotide sequence of regulator: 1593 bp    .

>REG0316 NZ_KQ410497:c213851-212259 [Vibrio cholerae V52]
ATGAGTCAATGGCTGGCGTTTGCAACCCAATTGGTGGGAGTAAGGAAGTCCCACCAGCTTGCGCTGCAATTTGTTGATCT
GCTGACTCAAGGCTTAGATCTGAGTGATAGCCTTTTGTTGTTGCCATCGTCGGATGGGCGTTTACTTGTGCCTCATGATC
CACAGCGTCAGTTCGCGTGGTCAGTGACGGATTTTGATGTGCCTTTTGCCCATGTTTTGCAGTCATCCAATGCGATGCAT
TTGACCGCGGAAGAGTTGGTATTTTGGCAATCCAATCGCACTTTTAGCCAGTTAACCTCTCGCGTGGGGATGTTTGATTC
GGTATGGATCCAACCCCTGCCGATGCATACTCGCCAAGTACACTCGATCTTGCTGTTGATGGGCGAGAGTCACGGCATCG
TGAGCGCTTTTGAAAACGCGGACTTTTTGAAGTTTATCGAGGTCTTTTCCCAGCAATGGTCACTGCTCAACGATATGGAA
CGTGAAGAGCAGCGCCGCTTGGAGCTGAAACAATCGCTGACGGATATTGAACGCGACTCGGCGCAGCGCTCACTGGCCAA
TGCGTTATCGCGTACTTTGATTGGTGAAAGCGCTGCGATGCAAAAGCTGCGTGAGCAGATTGTCTCTGCCGCTAACTCTC
AGCTCTCGGTCATGGTGCAAGGTGAAACTGGCACGGGTAAAGAGTTAGTCGCCGCGGCTGTGCATGAACTTTCGTCACGT
AAGTCTGCGCCGTTTGTTGCGATCAACTGTGCGGCGATCCCTGAGCATCTACTGGAAAGTGAACTGTTTGGCTACTGCAA
AGGGGCATTTTCTGGAGCGGACAGCGATAAACAGGGACTTATCGCACAAGCGAATGGTGGCACGCTATTTTTGGATGAGA
TCGGCGATATGCCGCTCACCTTACAAGCCAAATTATTGCGTGTGCTTGAATCACGTACTTTCCGACCGCTCGGTGGTAAG
CAGGAGCTCAGTTCGGATTTCCGTTTGGTATCGGCCACACACGTCAATCTGCTGGATCAAGTACGCAAAAAAGAATTTCG
CCAAGATCTCTATTACCGTCTATTCCAGTATCCGATCACCTTGCCGCGTTTGGCGGCACGCTTGGAAGATATTGAGCTGC
TCAGTGAGCACTTTGTGCGCGTGTTCAACTTGCAACATAACACGCGCATTCGTGGTTTGAACTATCGAGCCATCGACTGC
CTTAAGCAGTACGACTTCCCGGGAAATGTGCGTGAGCTGAAACATTTGATTGAGTTTGGCTGCGCGCAAACAGCAGATGG
TACGCAGGTGGAAGCTAGTTGCTTTGCCCATCGTTTACAAACCCTACCCTGTCTTGCGCCTGAAACGACGCCGGTAGCCG
TTTCTGTTGAAACTGAGAACGTGGATCTTGAACCCTCGGTTGCGCTGGCTGGAGAACCGAATTTTGCGGTTATCCATGAT
TTGAAACAGGCGGTCAGTCAGTTTGAAGCGTTGATCATCAGTGAGCGTTTGAACCGCTTTGCTGGCGATCGCGCGAAAGC
GGCGAAAAGTCTCGGTATCCCTAAGCGTACCTTGGCCTACAAGTGCCTGAAATTGGAGATCAAAACCCCATGA