SecReT6, T6SS database

Detailed information of regulator

Summary

Regulator ID	REG0369
Regulator type	protein
Regulatory pathway (from literatures)	TCS-RR
KEGG pathway (determined by BlastKOALA)	Two-component system (map02020)
Related T6SS	T6SS00790 (Type i1)
Strain	Vibrio cholerae V52
Replicon	chromosome
Sequence	Protein sequence (230 a.a.); Nucleotide sequence (690 bp)
Description	ΔwigR V52 was greatly impaired in its ability to kill E. coli prey. This reduced killing activity was reflected at both the protein and messenger RNA levels, as ΔwigR V52 produced fewer T6SS needles and had reduced expression of T6SS genes, hcp and vipA, compared with the WT.
Reference	[1] Hersch SJ, Watanabe N, Stietz MS, et al. Envelope stress responses defend against type six secretion system attacks independently of immunity proteins. Nat Microbiol. 2020 May;5(5):706-714. doi: 10.1038/s41564-020-0672-6. Epub 2020 Feb 24. PMID: 32094588 [2] Hersch SJ, Manera K, Dong TG. Defending against the Type Six Secretion System: beyond Immunity Genes. Cell Rep. 2020 Oct 13;33(2):108259. doi: 10.1016/j.celrep.2020.108259. PMID: 33053336

External database links

Locus tag (Gene)	VCV52_RS14135 (wigR/phoB/vxrB)
Coordinates (Strand)	201806..202495 (-)
NCBI ID	446013262
RefSeq	NZ_KQ410503
Uniprot ID	Q9KU11_VIBCH, A0A0X1KUP3_VIBCO, Q19LH9_VIBC3, D7HER4_VIBCL, A0A0K9UVD4_VIBCL, A0A0H3PW59_VIBCL, C3LSX2_VIBCM, A0A085TF90_VIBCL, D2YHP6_VIBMI, A0A1D8SDF5_VIBMI
KEGG ID	vcq:EN18_07005, vcx:VAA049_2973, vcm:VCM66_0677, vch:VC0719, vcz:VAB027_1380, vco:VC0395_A0253
KEGG pathway (determined by BlastKOALA)	map02020
PDB ID	-

Target

Target	Regulation (↓/↑)	Target sequence
T6SS transcription level	↓	-

Pfam domain hit(s) of regulator

Domain	Pfam ID	E-value	Aligned region
Response_reg	PF00072.26	5.7e-29	5..116
Trans_reg_C	PF00486.30	1.4e-28	151..225

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-229	MSRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDSAMSKLAEPFPDLVLLDWMLPGGSGINLIKHMKREEMTRNIPVVMLTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVIRRVTPTALEDVIDVQGLKLDPVSHRVTANDQPLDMGPTEFKMLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALEDAGHDKLIQTVRGAGYRFSTKA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9966	-	-

Protein sequence of regulator: 230 a.a. .

                                    >REG0369 NZ_KQ410503:c202495-201806 [Vibrio cholerae V52]
MSRRILVVEDEAPIREMLCFVLEQKGYQAVEAEDYDSAMSKLAEPFPDLVLLDWMLPGGSGINLIKHMKREEMTRNIPVV
MLTARGEEEDKVRGLEVGADDYITKPFSPKELVARLKAVIRRVTPTALEDVIDVQGLKLDPVSHRVTANDQPLDMGPTEF
KMLHFFMTHQERVYSREQLLNNVWGTNVYVEDRTVDVHIRRLRKALEDAGHDKLIQTVRGAGYRFSTKA*

                                 

Nucleotide sequence of regulator: 690 bp .

                                    >REG0369 NZ_KQ410503:c202495-201806 [Vibrio cholerae V52]
ATGTCTAGAAGGATTCTGGTTGTTGAAGACGAAGCACCGATTCGTGAAATGTTGTGTTTCGTGCTTGAACAAAAGGGCTA
TCAGGCGGTTGAGGCTGAAGATTATGATTCGGCGATGAGTAAGCTGGCTGAGCCTTTTCCTGATCTCGTCCTGCTTGACT
GGATGTTGCCTGGTGGTAGTGGCATCAACCTCATCAAGCATATGAAGCGCGAAGAGATGACGCGCAACATTCCTGTTGTG
ATGCTCACCGCCCGTGGTGAAGAAGAAGATAAAGTTCGCGGCTTAGAAGTGGGCGCGGATGATTACATTACCAAACCGTT
TTCACCTAAAGAGTTGGTTGCCCGTTTAAAAGCGGTGATCCGCCGTGTCACTCCGACGGCGTTGGAGGATGTGATTGATG
TTCAAGGTTTGAAGTTAGATCCGGTCTCACACCGTGTGACCGCTAACGATCAGCCGTTGGATATGGGACCGACTGAGTTC
AAGATGTTACACTTCTTCATGACACACCAAGAGCGTGTGTATAGCCGCGAGCAGCTACTCAATAATGTTTGGGGCACCAA
CGTGTATGTGGAAGATCGCACGGTAGACGTCCACATTCGTCGTCTACGTAAAGCCTTAGAAGACGCTGGACATGATAAGC
TGATCCAGACGGTGCGCGGCGCGGGGTATCGTTTTTCAACCAAAGCCTAA

                                