Detailed information of accessory protein
Summary
| Accessory protein ID | ACP44067  |
| Type | structure affiliated protein |
| Related T6SS (Type) | T6SS00790 (Type i1) |
| Strain | Vibrio cholerae V52 |
| Replicon | chromosome |
| Related structure protein | VCV52_RS01060 (tssE); VCV52_RS01055 (tssF); VCV52_RS01030 (tssL); VCV52_RS01005 (tssM) |
| Related effector | VCV52_RS06835 (vgrG1); VCV52_RS00990 (vgrG3); VCV52_RS01485 (hcp-2) |
| Description | TagA interact with multiple T6SS structural components. |
| Reference | [1] Stietz MS, Liang X, Li H, et al. TssA-TssM-TagA interaction modulates type VI secretion system sheath-tube assembly in Vibrio cholerae. Nat Commun. 2020 Oct 8;11(1):5065. doi: 10.1038/s41467-020-18807-9. PMID: 33033237 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | VCV52_RS01000 (tagA) |
| Coordinates (Strand) | 205313..206575 (-) |
| NCBI ID | WP_000073086.1 |
| RefSeq | NZ_KQ410497 |
| Uniprot ID | UPI00005F4E99 |
| KEGG ID | - |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| ImpA_N | PF06812.14 | 1.3e-08 | 25..99 |
| Cytochrom_B562 | PF07361.13 | 7.1e-05 | 327..397 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-200 | MSNVIFIDNVCYRLTNDSEEIRGLEPYAKVREEINRRFNPIAGGTDWAVVKEYCEQLACNQGMDFLICGYYAVACLKTQGLAGYATGMELMSASLANQGEYDVKSAKVRKEILDWVNARVVQELKALKPTYESLRDLYRAERHCERLHQLFEQQQTEYKVDFEGVGFALFEHIDRIETQYHSLLKKQEKAQPPKLKFWQR |
| Transmembrane helix | 201-223 | GYALFTLGVLGVALGVVGGYWGW |
| Outside | 224-420 | PWFYTTPYAAPQRITTLNEASQTQALLNDSSTSERARWQHDLIPLYRDTLQQNLTISFSEPKRHAITQLNLLRTLYPENEQVNALSQEFAIQQQQALEQTALFVAKFSEIRTKMANIALLAKRGKWAELEKQTKSLEEFAVSLSPIYGRVDYVQGLLEQGDVTNAQKEFAILKQRLDSLSWKIAELEQRIGDAALLQ |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9722 | - | - |
Protein sequence: 421 a.a.
>ACP44067 NZ_KQ410497:c206575-205313 [Vibrio cholerae V52]
MSNVIFIDNVCYRLTNDSEEIRGLEPYAKVREEINRRFNPIAGGTDWAVVKEYCEQLACNQGMDFLICGYYAVACLKTQG
LAGYATGMELMSASLANQGEYDVKSAKVRKEILDWVNARVVQELKALKPTYESLRDLYRAERHCERLHQLFEQQQTEYKV
DFEGVGFALFEHIDRIETQYHSLLKKQEKAQPPKLKFWQRGYALFTLGVLGVALGVVGGYWGWPWFYTTPYAAPQRITTL
NEASQTQALLNDSSTSERARWQHDLIPLYRDTLQQNLTISFSEPKRHAITQLNLLRTLYPENEQVNALSQEFAIQQQQAL
EQTALFVAKFSEIRTKMANIALLAKRGKWAELEKQTKSLEEFAVSLSPIYGRVDYVQGLLEQGDVTNAQKEFAILKQRLD
SLSWKIAELEQRIGDAALLQ*
MSNVIFIDNVCYRLTNDSEEIRGLEPYAKVREEINRRFNPIAGGTDWAVVKEYCEQLACNQGMDFLICGYYAVACLKTQG
LAGYATGMELMSASLANQGEYDVKSAKVRKEILDWVNARVVQELKALKPTYESLRDLYRAERHCERLHQLFEQQQTEYKV
DFEGVGFALFEHIDRIETQYHSLLKKQEKAQPPKLKFWQRGYALFTLGVLGVALGVVGGYWGWPWFYTTPYAAPQRITTL
NEASQTQALLNDSSTSERARWQHDLIPLYRDTLQQNLTISFSEPKRHAITQLNLLRTLYPENEQVNALSQEFAIQQQQAL
EQTALFVAKFSEIRTKMANIALLAKRGKWAELEKQTKSLEEFAVSLSPIYGRVDYVQGLLEQGDVTNAQKEFAILKQRLD
SLSWKIAELEQRIGDAALLQ*
Nucleotide sequence: 1263 bp
>ACP44067 NZ_KQ410497:c206575-205313 [Vibrio cholerae V52]
ATGTCCAATGTGATTTTTATTGATAACGTTTGCTACCGATTAACCAATGATTCTGAGGAGATTCGCGGATTAGAGCCCTA
TGCGAAGGTTCGTGAGGAGATAAACCGCCGCTTCAATCCGATTGCTGGGGGCACGGATTGGGCAGTGGTAAAAGAGTACT
GTGAACAACTGGCCTGTAACCAAGGAATGGATTTTCTGATCTGCGGTTACTATGCGGTAGCCTGCCTGAAAACTCAGGGG
TTAGCGGGGTATGCCACCGGTATGGAGCTGATGAGTGCAAGCCTTGCCAACCAAGGTGAGTATGATGTGAAAAGCGCCAA
AGTGCGCAAAGAGATCCTTGATTGGGTCAATGCACGTGTGGTGCAGGAACTGAAAGCACTTAAACCAACTTATGAGTCGC
TGCGAGATCTGTATCGGGCTGAGCGCCATTGCGAGCGGTTACATCAGCTGTTTGAACAGCAACAAACCGAATACAAAGTA
GACTTCGAAGGGGTCGGGTTTGCTCTGTTTGAACACATAGACAGAATTGAAACCCAATACCACAGCTTATTGAAAAAGCA
GGAGAAGGCTCAGCCACCTAAGCTTAAGTTTTGGCAGCGCGGTTACGCGTTGTTCACTCTAGGTGTACTTGGGGTTGCAC
TGGGAGTGGTGGGAGGCTACTGGGGGTGGCCTTGGTTTTATACCACGCCTTATGCCGCGCCACAGCGCATCACCACGTTG
AATGAGGCCTCTCAAACTCAAGCACTGCTCAACGACTCTTCCACAAGTGAGCGTGCGCGTTGGCAACATGATCTGATCCC
GCTGTATCGCGATACTTTGCAGCAGAATTTAACCATCTCGTTTAGTGAACCTAAGCGACATGCCATCACTCAGCTGAATC
TATTGCGCACTCTTTATCCTGAAAACGAGCAAGTGAATGCACTGAGTCAAGAGTTTGCCATTCAGCAGCAACAAGCGTTG
GAGCAAACCGCGCTGTTCGTGGCTAAGTTCAGCGAAATTCGCACCAAAATGGCCAATATCGCGTTACTGGCTAAGCGAGG
GAAATGGGCTGAGTTAGAAAAGCAGACCAAATCATTAGAAGAGTTCGCGGTCAGCCTATCACCGATTTATGGTCGAGTTG
ACTATGTGCAAGGCCTACTTGAACAGGGCGATGTCACAAATGCCCAGAAAGAGTTTGCGATCCTCAAACAAAGGCTAGAT
AGCCTAAGTTGGAAAATTGCCGAGCTTGAGCAGCGTATCGGCGATGCGGCTTTGTTGCAGTGA
ATGTCCAATGTGATTTTTATTGATAACGTTTGCTACCGATTAACCAATGATTCTGAGGAGATTCGCGGATTAGAGCCCTA
TGCGAAGGTTCGTGAGGAGATAAACCGCCGCTTCAATCCGATTGCTGGGGGCACGGATTGGGCAGTGGTAAAAGAGTACT
GTGAACAACTGGCCTGTAACCAAGGAATGGATTTTCTGATCTGCGGTTACTATGCGGTAGCCTGCCTGAAAACTCAGGGG
TTAGCGGGGTATGCCACCGGTATGGAGCTGATGAGTGCAAGCCTTGCCAACCAAGGTGAGTATGATGTGAAAAGCGCCAA
AGTGCGCAAAGAGATCCTTGATTGGGTCAATGCACGTGTGGTGCAGGAACTGAAAGCACTTAAACCAACTTATGAGTCGC
TGCGAGATCTGTATCGGGCTGAGCGCCATTGCGAGCGGTTACATCAGCTGTTTGAACAGCAACAAACCGAATACAAAGTA
GACTTCGAAGGGGTCGGGTTTGCTCTGTTTGAACACATAGACAGAATTGAAACCCAATACCACAGCTTATTGAAAAAGCA
GGAGAAGGCTCAGCCACCTAAGCTTAAGTTTTGGCAGCGCGGTTACGCGTTGTTCACTCTAGGTGTACTTGGGGTTGCAC
TGGGAGTGGTGGGAGGCTACTGGGGGTGGCCTTGGTTTTATACCACGCCTTATGCCGCGCCACAGCGCATCACCACGTTG
AATGAGGCCTCTCAAACTCAAGCACTGCTCAACGACTCTTCCACAAGTGAGCGTGCGCGTTGGCAACATGATCTGATCCC
GCTGTATCGCGATACTTTGCAGCAGAATTTAACCATCTCGTTTAGTGAACCTAAGCGACATGCCATCACTCAGCTGAATC
TATTGCGCACTCTTTATCCTGAAAACGAGCAAGTGAATGCACTGAGTCAAGAGTTTGCCATTCAGCAGCAACAAGCGTTG
GAGCAAACCGCGCTGTTCGTGGCTAAGTTCAGCGAAATTCGCACCAAAATGGCCAATATCGCGTTACTGGCTAAGCGAGG
GAAATGGGCTGAGTTAGAAAAGCAGACCAAATCATTAGAAGAGTTCGCGGTCAGCCTATCACCGATTTATGGTCGAGTTG
ACTATGTGCAAGGCCTACTTGAACAGGGCGATGTCACAAATGCCCAGAAAGAGTTTGCGATCCTCAAACAAAGGCTAGAT
AGCCTAAGTTGGAAAATTGCCGAGCTTGAGCAGCGTATCGGCGATGCGGCTTTGTTGCAGTGA