SecReT6, T6SS database

Detailed information of effector

Summary

Effector ID	EFF76632
Effector type	-
Cognate immunity protein	-
Related T6SS (Type)	T6SS00790 (Type i1)
Strain	Vibrio cholerae V52
Replicon	chromosome
Sequence	Protein sequence (173 a.a.); Nucleotide sequence (519 bp)
Description	-
Reference	[1] Ishikawa T, Sabharwal D, Bröms J, et al. Pathoadaptive conditional regulation of the type VI secretion system in Vibrio cholerae O1 strains. Infect Immun. 2012 Feb;80(2):575-84. doi: 10.1128/IAI.05510-11. Epub 2011 Nov 14. PMID: 22083711 [2] Ishikawa T, Rompikuntal PK, Lindmark B, et al. Quorum sensing regulation of the two hcp alleles in Vibrio cholerae O1 strains. PLoS One. 2009 Aug 24;4(8):e6734. doi: 10.1371/journal.pone.0006734. PMID: 19701456 [3] Stietz MS, Liang X, Li H, et al. TssA-TssM-TagA interaction modulates type VI secretion system sheath-tube assembly in Vibrio cholerae. Nat Commun. 2020 Oct 8;11(1):5065. doi: 10.1038/s41467-020-18807-9. PMID: 33033237

Experimental investigation has been performed.

Bioinformatics investigation has been performed

External database links

Locus tag (Gene)	VCV52_RS06840 (hcp2)
Coordinates (Strand)	438678..439196 (-)
NCBI ID	WP_001142947.1
RefSeq	NZ_KQ410498
Uniprot ID	A0A366AGP5_9VIBR, H9L4Q3_VIBCH, A0A0X1J838_VIBCO, D7HBV7_VIBCL, A0A0K9V0F0_VIBCL, A0A7U8WQB7_VIBCL, A0A0H3PYB9_VIBCL, C3LMB0_VIBCM, P72350_VIBCL
KEGG ID	vcq:EN18_03855, vcz:VAB027_2088, vcx:VAA049_1881, vch:VC1415, vcm:VCM66_1370, vcm:VCM66_A0016, vch:VCA0017, vcz:VAB027_1005, vcq:EN18_10295
PDB ID	5MXN, 5OJQ

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_HCP	PF05638.14	5.3e-35	6..155

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-172	MPTPCYISIEGQTQGLITAGACTADSIGDSFVEGHEDEMLVQQFDHVVTVPTDPQSGQPSGQRVHKPFKFTVALNKAVPLLYNALSSGEKLKTVELKWYRTSIEGKQENFFTTKLENASIVDIHCEMPHCQDPAKSDFTQNVTVSLSYRKITWDHVNAGTSGSDDWRKPIEA

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.7783	-	-

Protein sequence: 173 a.a. .

                                    >EFF76632 NZ_KQ410498:c439196-438678  (Unknown) [Vibrio cholerae V52]
MPTPCYISIEGQTQGLITAGACTADSIGDSFVEGHEDEMLVQQFDHVVTVPTDPQSGQPSGQRVHKPFKFTVALNKAVPL
LYNALSSGEKLKTVELKWYRTSIEGKQENFFTTKLENASIVDIHCEMPHCQDPAKSDFTQNVTVSLSYRKITWDHVNAGT
SGSDDWRKPIEA*

                                

Nucleotide sequence: 519 bp .

                                    >EFF76632 NZ_KQ410498:c439196-438678  (Unknown) [Vibrio cholerae V52]
ATGCCAACTCCATGTTATATCTCTATCGAAGGCCAAACTCAGGGTCTTATCACTGCAGGTGCATGTACTGCTGACTCTAT
CGGCGATTCATTCGTTGAAGGCCACGAAGATGAGATGCTGGTTCAGCAGTTTGACCACGTTGTGACTGTACCGACTGACC
CACAATCTGGTCAGCCTTCAGGCCAACGTGTGCACAAGCCATTCAAATTCACTGTGGCGCTGAACAAAGCGGTTCCTCTG
CTGTACAACGCGCTCTCTTCTGGTGAGAAGCTGAAAACCGTTGAGCTGAAGTGGTACCGTACCTCTATCGAAGGTAAACA
AGAGAACTTCTTCACCACTAAGCTGGAAAACGCGTCTATCGTCGACATCCACTGTGAAATGCCACACTGCCAAGACCCAG
CAAAATCTGACTTTACTCAGAATGTGACTGTGTCTCTGTCTTACCGCAAAATCACTTGGGACCACGTTAACGCGGGCACC
TCAGGCTCTGATGACTGGCGTAAGCCAATCGAAGCGTAA

                                