Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   AAEQ75_RS12445 Genome accession   NZ_CP154631
Coordinates   2632136..2632321 (+) Length   61 a.a.
NCBI ID   WP_343349101.1    Uniprot ID   -
Organism   Pseudomonas sediminis strain I4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 2627136..2637321
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEQ75_RS12425 (AAEQ75_12425) coaE 2627324..2627932 (-) 609 WP_343349093.1 dephospho-CoA kinase -
  AAEQ75_RS12430 (AAEQ75_12430) pilD 2628110..2628976 (-) 867 WP_343349095.1 A24 family peptidase Machinery gene
  AAEQ75_RS12435 (AAEQ75_12435) pilC 2628980..2630200 (-) 1221 WP_343349097.1 type II secretion system F family protein Machinery gene
  AAEQ75_RS12440 (AAEQ75_12440) pilB 2630204..2631904 (-) 1701 WP_343349099.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAEQ75_RS12445 (AAEQ75_12445) pilA 2632136..2632321 (+) 186 WP_343349101.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AAEQ75_RS12450 (AAEQ75_12450) - 2632333..2632776 (+) 444 WP_343349103.1 hypothetical protein -
  AAEQ75_RS12455 (AAEQ75_12455) - 2632878..2633033 (+) 156 WP_343349105.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AAEQ75_RS12460 (AAEQ75_12460) - 2633095..2633901 (+) 807 WP_343349106.1 hypothetical protein -
  AAEQ75_RS12465 (AAEQ75_12465) - 2633904..2634743 (+) 840 WP_343352379.1 hypothetical protein -
  AAEQ75_RS12470 (AAEQ75_12470) - 2634740..2635651 (+) 912 WP_343349108.1 ABC transporter ATP-binding protein -
  AAEQ75_RS12475 (AAEQ75_12475) - 2635905..2636303 (+) 399 WP_343349110.1 nucleotidyltransferase domain-containing protein -
  AAEQ75_RS12480 (AAEQ75_12480) - 2636293..2636715 (+) 423 WP_143506146.1 DUF86 domain-containing protein -

Sequence


Protein


Download         Length: 61 a.a.        Molecular weight: 6608.86 Da        Isoelectric Point: 10.2806

>NTDB_id=993861 AAEQ75_RS12445 WP_343349101.1 2632136..2632321(+) (pilA) [Pseudomonas sediminis strain I4]
MKSLKKQKGFTLIELMIVVAIIGILAAIAIPQFNEYRAKSNDTVSNADTKNTLTVMAAAQL

Nucleotide


Download         Length: 186 bp        

>NTDB_id=993861 AAEQ75_RS12445 WP_343349101.1 2632136..2632321(+) (pilA) [Pseudomonas sediminis strain I4]
ATGAAGTCTCTGAAGAAGCAAAAGGGTTTTACCCTGATCGAGCTGATGATCGTAGTCGCCATCATCGGTATTCTGGCTGC
TATCGCCATTCCGCAGTTCAATGAATATCGCGCCAAGTCCAACGATACCGTCTCTAATGCGGATACTAAAAATACTCTGA
CCGTTATGGCTGCTGCTCAGCTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

59.615

85.246

0.508

  pilA Vibrio parahaemolyticus RIMD 2210633

60

81.967

0.492

  pilA Acinetobacter baumannii strain A118

81.081

60.656

0.492

  pilA Pseudomonas aeruginosa PAK

78.947

62.295

0.492

  pilA2 Legionella pneumophila strain ERS1305867

65.217

75.41

0.492

  pilA2 Legionella pneumophila str. Paris

65.217

75.41

0.492

  pilA/pilA1 Eikenella corrodens VA1

70.732

67.213

0.475

  pilA Acinetobacter nosocomialis M2

78.378

60.656

0.475

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

77.778

59.016

0.459

  pilA Vibrio cholerae strain A1552

77.778

59.016

0.459

  pilA Vibrio cholerae C6706

77.778

59.016

0.459

  pilA/pilAI Pseudomonas stutzeri DSM 10701

77.143

57.377

0.443

  pilA Vibrio campbellii strain DS40M4

75

59.016

0.443

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

67.5

65.574

0.443

  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50.943

86.885

0.443

  pilA/pilAII Pseudomonas stutzeri DSM 10701

63.415

67.213

0.426

  pilA/pilA4 Thermus thermophilus HB27

58.537

67.213

0.393

  comF Acinetobacter baylyi ADP1

63.158

62.295

0.393

  pilE Legionella pneumophila strain ERS1305867

44.231

85.246

0.377

  pilE Legionella pneumophila str. Paris

44.231

85.246

0.377

  pilA Haemophilus influenzae 86-028NP

67.647

55.738

0.377

  pilE Neisseria gonorrhoeae MS11

67.647

55.738

0.377

  pilE Neisseria gonorrhoeae strain FA1090

67.647

55.738

0.377

  pilA Haemophilus influenzae Rd KW20

67.647

55.738

0.377

  comE Acinetobacter baylyi ADP1

62.162

60.656

0.377

  pilA Glaesserella parasuis strain SC1401

66.667

54.098

0.361

  pilL Neisseria gonorrhoeae MS11

62.857

57.377

0.361


Multiple sequence alignment