Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AAEQ75_RS12440 Genome accession   NZ_CP154631
Coordinates   2630204..2631904 (-) Length   566 a.a.
NCBI ID   WP_343349099.1    Uniprot ID   -
Organism   Pseudomonas sediminis strain I4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2625204..2636904
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AAEQ75_RS12405 (AAEQ75_12405) - 2625247..2625876 (-) 630 WP_125880112.1 DUF1780 domain-containing protein -
  AAEQ75_RS12410 (AAEQ75_12410) - 2626074..2626763 (+) 690 WP_343349088.1 energy-coupling factor ABC transporter permease -
  AAEQ75_RS12415 (AAEQ75_12415) - 2626870..2627085 (+) 216 WP_343349090.1 hypothetical protein -
  AAEQ75_RS12420 (AAEQ75_12420) yacG 2627133..2627327 (-) 195 WP_256835435.1 DNA gyrase inhibitor YacG -
  AAEQ75_RS12425 (AAEQ75_12425) coaE 2627324..2627932 (-) 609 WP_343349093.1 dephospho-CoA kinase -
  AAEQ75_RS12430 (AAEQ75_12430) pilD 2628110..2628976 (-) 867 WP_343349095.1 A24 family peptidase Machinery gene
  AAEQ75_RS12435 (AAEQ75_12435) pilC 2628980..2630200 (-) 1221 WP_343349097.1 type II secretion system F family protein Machinery gene
  AAEQ75_RS12440 (AAEQ75_12440) pilB 2630204..2631904 (-) 1701 WP_343349099.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AAEQ75_RS12445 (AAEQ75_12445) pilA 2632136..2632321 (+) 186 WP_343349101.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AAEQ75_RS12450 (AAEQ75_12450) - 2632333..2632776 (+) 444 WP_343349103.1 hypothetical protein -
  AAEQ75_RS12455 (AAEQ75_12455) - 2632878..2633033 (+) 156 WP_343349105.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AAEQ75_RS12460 (AAEQ75_12460) - 2633095..2633901 (+) 807 WP_343349106.1 hypothetical protein -
  AAEQ75_RS12465 (AAEQ75_12465) - 2633904..2634743 (+) 840 WP_343352379.1 hypothetical protein -
  AAEQ75_RS12470 (AAEQ75_12470) - 2634740..2635651 (+) 912 WP_343349108.1 ABC transporter ATP-binding protein -
  AAEQ75_RS12475 (AAEQ75_12475) - 2635905..2636303 (+) 399 WP_343349110.1 nucleotidyltransferase domain-containing protein -
  AAEQ75_RS12480 (AAEQ75_12480) - 2636293..2636715 (+) 423 WP_143506146.1 DUF86 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62143.04 Da        Isoelectric Point: 5.3341

>NTDB_id=993860 AAEQ75_RS12440 WP_343349099.1 2630204..2631904(-) (pilB) [Pseudomonas sediminis strain I4]
MSDTAQLTGLARQLVNANLLDERAASQAFAQALRNRIPLVSYLVQNKLATSRALAEIASEQFGVPYFDLAAIDKDSQPRE
LISEKLCRQHRVLPLYRRGNKLFIALSDPTNHQAITDVQFSTGLSTDALLVEDDKLGDALDKFFDAGTSGMEELADVDLD
GLDVEAVSDEADDAGAGNDSDDAPVVRFINKMLLDAIKGGSSDLHFEPYEKAYRVRFRTDGMLHEVARPPIQLASRLSAR
LKVMAQLDISERRKPQDGRIKMKVSKTKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEEQKELYMTALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPKHGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSCKKEVTLPREALLKEGFPE
SKIGSFKLFAPNGCDNCKGGYKGRVGIYEVVKITPSLQRIIMEEGNSIEIAAQAKADGFNDLRTSGLFKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=993860 AAEQ75_RS12440 WP_343349099.1 2630204..2631904(-) (pilB) [Pseudomonas sediminis strain I4]
ATGAGCGACACCGCCCAACTGACCGGCCTCGCCCGCCAACTGGTCAATGCCAACCTGCTCGACGAGCGCGCCGCCAGCCA
GGCCTTTGCCCAGGCGCTGCGCAACAGGATCCCCCTGGTGTCCTACCTGGTGCAGAACAAGCTGGCCACCAGCCGCGCCC
TGGCCGAGATCGCCTCCGAGCAGTTCGGCGTGCCCTACTTCGACCTGGCGGCCATCGACAAGGACAGCCAGCCGCGCGAG
TTGATCAGCGAGAAGCTGTGCCGCCAGCACCGCGTGCTGCCGCTGTACCGCCGCGGCAACAAGCTGTTTATCGCCCTCTC
CGACCCCACCAACCACCAGGCCATCACCGACGTGCAGTTCAGCACCGGGCTGAGCACCGACGCCCTGCTGGTGGAGGACG
ACAAGCTGGGCGACGCCCTGGACAAGTTCTTCGACGCCGGCACCAGCGGTATGGAAGAGCTGGCCGACGTCGATCTGGAC
GGGCTTGATGTCGAAGCCGTCAGTGACGAAGCGGACGATGCTGGCGCCGGTAACGACTCTGACGATGCGCCAGTGGTGCG
CTTCATCAACAAGATGCTGCTGGACGCCATCAAGGGTGGCTCGTCCGACCTGCACTTCGAGCCCTACGAAAAAGCCTACC
GCGTCCGCTTTCGTACCGACGGCATGCTGCATGAAGTAGCCCGCCCGCCCATCCAGCTGGCCAGCCGACTTTCCGCTCGC
CTCAAGGTCATGGCGCAACTGGATATCTCCGAGCGGCGCAAGCCGCAGGACGGCCGGATCAAGATGAAGGTCTCCAAAAC
CAAAGCCATCGACTTTCGCGTCAACACCCTGCCCACCCTCTGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCCACCA
GCGCGCAGATGGGCATCGATGCGCTGGGCTATGAAGAAGAACAGAAAGAGCTGTACATGACAGCTCTGAAGCAGCCGCAA
GGCATGATTCTGGTGACCGGCCCAACCGGCTCGGGCAAGACGGTATCGCTTTATACCGGCCTGAATATTCTCAACACCAC
GGACATCAATATTTCCACCGCGGAAGACCCGGTGGAAATCAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGAAAC
ACGGCATGGATTTCGCCCAGGCCCTGCGCGCCTTCCTGCGCCAGGACCCGGATGTGATCATGGTCGGTGAGATTCGCGAC
CTGGAAACGGCCGAAATCGCCATCAAGGCCGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
GGAAACCCTGACCCGCCTGCGCAACATGGGCGTGCCGGCATTCAACATCGCCACCTCGGTGAACCTGATCATCGCCCAGC
GCCTGGCGCGTAAACTTTGCAGTTGCAAGAAGGAAGTAACACTGCCGCGCGAGGCCTTGCTTAAAGAAGGCTTCCCTGAA
AGCAAGATTGGCAGCTTCAAGCTGTTCGCCCCCAATGGCTGTGACAATTGCAAAGGCGGCTATAAAGGAAGGGTGGGGAT
TTATGAAGTAGTTAAAATCACGCCCTCCCTGCAACGCATTATCATGGAGGAAGGCAACTCCATCGAAATCGCCGCGCAGG
CGAAAGCCGACGGCTTCAATGACCTCCGAACTTCCGGGCTGTTCAAGGCGATGCAGGGCATCACCAGCCTGGAAGAAGTC
AACCGTGTGACCAAGGACTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.474

100

0.599
  pilB Acinetobacter baylyi ADP1

59.043

99.647

0.588
  pilB Legionella pneumophila strain ERS1305867

54.24

100

0.542
  pilB Vibrio campbellii strain DS40M4

49.734

99.47

0.495
  pilB Vibrio parahaemolyticus RIMD 2210633

52.345

94.17

0.493
  pilF Neisseria gonorrhoeae MS11

49.293

100

0.493
  pilB Vibrio cholerae strain A1552

52.256

93.993

0.491
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.19

92.756

0.373
  pilF Thermus thermophilus HB27

37.276

98.587

0.367

Multiple sequence alignment