Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   P1P91_RS08805 Genome accession   NZ_CP119391
Coordinates   1874370..1874501 (-) Length   43 a.a.
NCBI ID   WP_311885756.1    Uniprot ID   -
Organism   Halomonas piscis strain SG2L-4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1866822..1875019 1874370..1874501 within 0


Gene organization within MGE regions


Location: 1866822..1875019
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P1P91_RS08765 (P1P91_08755) - 1867079..1867852 (-) 774 WP_311882012.1 IS110 family transposase -
  P1P91_RS08770 (P1P91_08760) - 1868077..1868715 (-) 639 WP_311882014.1 DUF2335 domain-containing protein -
  P1P91_RS08775 (P1P91_08765) - 1869127..1870506 (-) 1380 WP_311882016.1 sigma-54 dependent transcriptional regulator -
  P1P91_RS08780 (P1P91_08770) - 1871443..1871721 (-) 279 WP_311882017.1 transposase -
  P1P91_RS08785 (P1P91_08775) - 1872038..1872316 (-) 279 WP_311882017.1 transposase -
  P1P91_RS08790 (P1P91_08780) - 1872633..1873667 (-) 1035 WP_311882018.1 IS110 family transposase -
  P1P91_RS08800 (P1P91_08790) - 1874110..1874373 (-) 264 WP_311885754.1 hypothetical protein -
  P1P91_RS08805 (P1P91_08795) pilA 1874370..1874501 (-) 132 WP_311885756.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  P1P91_RS08810 (P1P91_08800) - 1874564..1875019 (-) 456 WP_311882019.1 pilin -

Sequence

Protein


Download         Length: 43 a.a.        Molecular weight: 4852.88 Da        Isoelectric Point: 12.6293

>NTDB_id=798776 P1P91_RS08805 WP_311885756.1 1874370..1874501(-) (pilA) [Halomonas piscis strain SG2L-4]
MQSRQRGFTLIELMIVVAIIGVLAAVAIPRYRGHVARHRPAKR

Nucleotide


Download         Length: 132 bp        

>NTDB_id=798776 P1P91_RS08805 WP_311885756.1 1874370..1874501(-) (pilA) [Halomonas piscis strain SG2L-4]
ATGCAATCCAGGCAGCGCGGCTTTACCCTGATCGAGCTGATGATCGTTGTTGCCATTATCGGCGTGCTGGCCGCCGTGGC
CATTCCCCGGTATCGAGGCCACGTGGCCCGGCACAGGCCAGCGAAGCGCTGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

72.973

86.047

0.628
  pilA Vibrio parahaemolyticus RIMD 2210633

75

83.721

0.628
  pilA2 Legionella pneumophila str. Paris

72.222

83.721

0.605
  pilA2 Legionella pneumophila strain ERS1305867

72.222

83.721

0.605
  pilA/pilAII Pseudomonas stutzeri DSM 10701

58.537

95.349

0.558
  pilA Vibrio campbellii strain DS40M4

66.667

83.721

0.558
  pilE Neisseria gonorrhoeae strain FA1090

64.865

86.047

0.558
  pilA/pilAI Pseudomonas stutzeri DSM 10701

64.865

86.047

0.558
  pilE Neisseria gonorrhoeae MS11

64.865

86.047

0.558
  pilA Acinetobacter nosocomialis M2

63.158

88.372

0.558
  pilA Vibrio cholerae C6706

70.588

79.07

0.558
  pilA Vibrio cholerae strain A1552

70.588

79.07

0.558
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

70.588

79.07

0.558
  comP Acinetobacter baylyi ADP1

68.571

81.395

0.558
  pilA Ralstonia pseudosolanacearum GMI1000

68.571

81.395

0.558
  pilA Acinetobacter baumannii strain A118

68.571

81.395

0.558
  pilA/pilA1 Eikenella corrodens VA1

62.162

86.047

0.535
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

58.974

90.698

0.535
  comF Acinetobacter baylyi ADP1

66.667

76.744

0.512
  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

59.459

86.047

0.512
  comE Acinetobacter baylyi ADP1

56.757

86.047

0.488
  pilA/pilA1 Synechocystis sp. PCC 6803

58.333

83.721

0.488
  pilA Haemophilus influenzae 86-028NP

55.556

83.721

0.465
  pilA Haemophilus influenzae Rd KW20

54.054

86.047

0.465
  pilL Neisseria gonorrhoeae MS11

54.545

76.744

0.419
  pilX Neisseria meningitidis 8013

54.545

76.744

0.419
  pilH Neisseria gonorrhoeae MS11

47.368

88.372

0.419
  comP Neisseria gonorrhoeae MS11

50

79.07

0.395
  comP Neisseria meningitidis 8013

50

79.07

0.395
  pilY2 Acinetobacter baumannii D1279779

50

74.419

0.372