Detailed information    

experimental Experimentally validated

Overview

Name   comP   Type   Machinery gene
Locus tag   NGFG_RS06520 Genome accession   NC_022240
Coordinates   1183915..1184364 (-) Length   149 a.a.
NCBI ID   WP_002214937.1    Uniprot ID   A0AA44U8B7
Organism   Neisseria gonorrhoeae MS11     
Function   DNA binding; DNA uptake; receptor of DNA uptake sequence (DUS)   
DNA binding and uptake

Function

ComP overexpression increases transformability by enhancing DNA uptake. ComP overexpression also enhances sequence-specific DNA binding in the absence of uptake.


Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1180402..1191652 1183915..1184364 within 0


Gene organization within MGE regions


Location: 1180402..1191652
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NGFG_RS16035 - 1180402..1180998 (-) 597 WP_012503916.1 TIGR02391 family protein -
  NGFG_RS16040 - 1181188..1182045 (-) 858 WP_012503917.1 ATP-binding protein -
  NGFG_RS06505 - 1182059..1182430 (-) 372 WP_012503918.1 DNA cytosine methyltransferase -
  NGFG_RS06510 - 1182427..1183017 (-) 591 WP_080229150.1 DNA cytosine methyltransferase -
  NGFG_RS06515 (NGFG_02183) - 1183392..1183538 (+) 147 WP_003691757.1 hypothetical protein -
  NGFG_RS06520 (NGFG_02184) comP 1183915..1184364 (-) 450 WP_002214937.1 type IV pilin protein Machinery gene
  NGFG_RS06525 (NGFG_02391) comE 1184452..1184847 (-) 396 WP_003703428.1 helix-hairpin-helix domain-containing protein Machinery gene
  NGFG_RS16045 - 1184962..1185067 (-) 106 Protein_1237 competence protein ComE -
  NGFG_RS14520 (NGFG_01985) - 1190701..1191354 (-) 654 Protein_1238 TIGR01621 family pseudouridine synthase -
  NGFG_RS14525 (NGFG_01986) - 1191308..1191652 (-) 345 WP_003691787.1 HAD hydrolase family protein -

Regulatory network

Positive effect      
Negative effect
Regulator Target Regulation
  pilV comP negative effect

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 16834.81 Da        Isoelectric Point: 9.7951

>NTDB_id=1117 NGFG_RS06520 WP_002214937.1 1183915..1184364(-) (comP) [Neisseria gonorrhoeae MS11]
MTDNRGFTLVELISVVLILSVLALIVYPSYRNYVEKAKINAVRAALLENAHFMEKFYLQNGRFKQTSTKWPSLPIKEAEG
FCIRLNGIARGALDSKFMLKAVAIDKDKNPFIIKMNENLVTFICKKSASSCSDGLDYFKGNDKDCKLLK

Nucleotide


Download         Length: 450 bp        

>NTDB_id=1117 NGFG_RS06520 WP_002214937.1 1183915..1184364(-) (comP) [Neisseria gonorrhoeae MS11]
ATGACTGATAATCGGGGGTTTACGCTGGTTGAATTAATATCAGTGGTCTTGATATTGTCTGTACTTGCTTTAATTGTTTA
TCCGAGCTATCGCAATTATGTTGAGAAAGCAAAGATAAATGCAGTGCGGGCAGCCTTGTTAGAAAATGCACATTTTATGG
AAAAGTTTTATCTGCAGAATGGGAGATTTAAACAAACATCTACCAAATGGCCAAGTTTGCCGATTAAAGAGGCAGAAGGC
TTTTGTATCCGTTTGAATGGAATCGCGCGCGGGGCTTTAGACAGTAAATTCATGTTGAAGGCGGTAGCCATAGATAAAGA
TAAAAATCCTTTTATTATTAAGATGAATGAAAATCTAGTAACCTTTATTTGCAAGAAGTCCGCCAGTTCGTGTAGTGACG
GGCTGGATTATTTTAAAGGAAATGATAAGGACTGCAAGTTACTTAAGTAG

Domains

Predicted by InterproScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(28-107)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comP Neisseria meningitidis 8013

99.329

100

0.993
  comP Neisseria subflava NJ9703

49.66

98.658

0.49

Multiple sequence alignment    



References

[1] Stian Aleksander Helsem et al. (2025) Two different and robustly modeled DNA binding modes of Competence Protein ComP - systematic modeling with AlphaFold 3, RoseTTAFold2NA, Chai-1 and re-docking in HADDOCK. PloS One 20(5):e0315160. [PMID: 40338866]
[2] Finn Erik Aas et al. (2002) Competence for natural transformation in Neisseria gonorrhoeae: components of DNA binding and uptake linked to type IV pilus expression. Molecular Microbiology 46(3):749-60. [PMID: 12410832]
[3] M Wolfgang et al. (1999) The comP locus of Neisseria gonorrhoeae encodes a type IV prepilin that is dispensable for pilus biogenesis but essential for natural transformation. Molecular Microbiology 31(5):1345-57. [PMID: 10200956]