Detailed information    

experimental Experimentally validated

Overview

Name   comP   Type   Machinery gene
Locus tag   NEISUBOT_RS06510 Genome accession   NZ_ACEO02000007
Coordinates   4968..5462 (+) Length   164 a.a.
NCBI ID   WP_050767750.1    Uniprot ID   -
Organism   Neisseria subflava NJ9703     
Function   DNA binding; DNA uptake; receptor of DNA uptake sequence (DUS)   
DNA binding and uptake

Function

The ComP homolog in the N. subflava commensal preferentially binds its cognate DUS and mediates DUS-enhanced transformation when expressed in N. meningitidis.


Genomic Context

Location: 1..10462
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NEISUBOT_RS06505 (NEISUBOT_04549) comE 4492..4779 (+) 288 WP_004520163.1 ComEA family DNA-binding protein Machinery gene
  NEISUBOT_RS06510 (NEISUBOT_04550) comP 4968..5462 (+) 495 WP_050767750.1 type IV pilin protein Machinery gene
  NEISUBOT_RS06515 (NEISUBOT_04552) pyrB 6505..7425 (+) 921 WP_039862268.1 aspartate carbamoyltransferase -
  NEISUBOT_RS06520 (NEISUBOT_04553) pyrI 7436..7894 (+) 459 WP_004520167.1 aspartate carbamoyltransferase regulatory subunit -
  NEISUBOT_RS06525 (NEISUBOT_04554) - 8088..8754 (+) 667 Protein_4 hypothetical protein -
  NEISUBOT_RS06530 (NEISUBOT_04555) - 9030..9893 (+) 864 WP_039862269.1 GNAT family N-acetyltransferase -
  NEISUBOT_RS12310 (NEISUBOT_04556) - 9917..10042 (+) 126 WP_003684698.1 hypothetical protein -

Sequence

Protein


Download         Length: 164 a.a.        Molecular weight: 18231.28 Da        Isoelectric Point: 10.0832

>NTDB_id=1187 NEISUBOT_RS06510 WP_050767750.1 4968..5462(+) (comP) [Neisseria subflava NJ9703]
MYLKAFDRKWDRATVQRGYSLIQLLVVMLLVSILATAAFTVYRESVRSANLRAAHAALLENARFMEQFYAKKGSFKLTST
KWPELPVKEAGGFCIRMSGQAKGILEGKFTLKAVALDREAEPRVLRLNESLTAVVCGKMKGKGSCTDGEEIFRGNDAECR
PFTG

Nucleotide


Download         Length: 495 bp        

>NTDB_id=1187 NEISUBOT_RS06510 WP_050767750.1 4968..5462(+) (comP) [Neisseria subflava NJ9703]
ATGTACTTAAAGGCATTTGACAGAAAATGGGATAGGGCAACTGTGCAGAGGGGCTACTCCTTGATACAGCTGCTGGTGGT
GATGCTGCTGGTTTCGATCTTGGCAACGGCTGCCTTTACGGTCTATCGGGAATCGGTCCGCTCGGCCAACCTGCGTGCAG
CGCATGCCGCGCTGCTGGAAAATGCGCGCTTTATGGAGCAGTTCTACGCGAAAAAGGGCAGCTTTAAGCTGACGTCGACG
AAGTGGCCGGAACTGCCGGTGAAGGAGGCGGGCGGTTTCTGTATCAGGATGAGCGGTCAGGCTAAGGGCATCCTGGAGGG
TAAGTTTACCTTGAAGGCGGTGGCGCTGGACAGGGAGGCGGAGCCGAGGGTGCTGCGCTTGAACGAGTCGCTGACGGCGG
TGGTGTGCGGGAAGATGAAGGGGAAGGGCAGCTGTACGGACGGTGAGGAGATATTTAGGGGCAATGATGCGGAGTGTCGG
CCTTTTACGGGGTGA

Domains

Predicted by InterproScan.

Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS)

(42-120)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comP Neisseria meningitidis 8013

49.664

100

0.497
  comP Neisseria gonorrhoeae MS11

49.66

98.658

0.49

Multiple sequence alignment    



References

[1] Jamie-Lee Berry et al. (2016) A Comparative Structure/Function Analysis of Two Type IV Pilin DNA Receptors Defines a Novel Mode of DNA Binding. Structure (London, England : 1993) 24(6):926-34. [PMID: 27161979]
[2] Jamie-Lee Berry et al. (2013) Functional analysis of the interdependence between DNA uptake sequence and its cognate ComP receptor during natural transformation in Neisseria species. PLoS Genetics 9(12):e1004014. [PMID: 24385921]