Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   M0M42_RS21130 Genome accession   NZ_CP096208
Coordinates   3536043..3536228 (+) Length   61 a.a.
NCBI ID   WP_282579612.1    Uniprot ID   -
Organism   Pseudomonas knackmussii strain B09-2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 3531043..3541228
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0M42_RS16495 (M0M42_16495) pilD 3531772..3532635 (-) 864 WP_248493403.1 A24 family peptidase Machinery gene
  M0M42_RS16500 (M0M42_16500) pilC 3532638..3533861 (-) 1224 WP_248493405.1 type II secretion system F family protein Machinery gene
  M0M42_RS16505 (M0M42_16505) pilB 3533864..3535570 (-) 1707 WP_248493407.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M0M42_RS21130 pilA 3536043..3536228 (+) 186 WP_282579612.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  M0M42_RS16515 (M0M42_16515) - 3536241..3536654 (+) 414 WP_248493410.1 hypothetical protein -
  M0M42_RS16520 (M0M42_16520) - 3536724..3537527 (+) 804 WP_248493412.1 ABC transporter permease subunit -
  M0M42_RS16525 (M0M42_16525) - 3537524..3538357 (+) 834 WP_248493414.1 hypothetical protein -
  M0M42_RS16530 (M0M42_16530) - 3538344..3539231 (+) 888 WP_248493416.1 ABC transporter ATP-binding protein -
  M0M42_RS16535 (M0M42_16535) - 3539838..3540278 (+) 441 WP_248493417.1 YtoQ family protein -

Sequence


Protein


Download         Length: 61 a.a.        Molecular weight: 6609.79 Da        Isoelectric Point: 10.1002

>NTDB_id=680431 M0M42_RS21130 WP_282579612.1 3536043..3536228(+) (pilA) [Pseudomonas knackmussii strain B09-2]
MKSIKKQKGFTLIELMIVVAIIGILAAIAIPQFQEYQAKGYDKSAQSDARNLLTAGIANSQ

Nucleotide


Download         Length: 186 bp        

>NTDB_id=680431 M0M42_RS21130 WP_282579612.1 3536043..3536228(+) (pilA) [Pseudomonas knackmussii strain B09-2]
ATGAAGTCCATCAAGAAGCAAAAGGGTTTTACCCTGATCGAACTGATGATCGTGGTTGCGATTATCGGCATTCTGGCTGC
GATCGCGATTCCTCAGTTTCAGGAATATCAGGCCAAAGGCTATGACAAGTCGGCCCAATCCGACGCTCGCAACCTTTTGA
CCGCCGGCATTGCGAACTCACAGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

52.113

100

0.607

  pilA Pseudomonas aeruginosa PAK

66.667

83.607

0.557

  pilA Acinetobacter baumannii strain A118

67.347

80.328

0.541

  pilA Acinetobacter nosocomialis M2

87.879

54.098

0.475

  pilA Vibrio parahaemolyticus RIMD 2210633

58

81.967

0.475

  pilA/pilAII Pseudomonas stutzeri DSM 10701

60.87

75.41

0.459

  pilA Vibrio campbellii strain DS40M4

77.778

59.016

0.459

  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.902

83.607

0.459

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

80

57.377

0.459

  pilA Vibrio cholerae strain A1552

80

57.377

0.459

  pilA Vibrio cholerae C6706

80

57.377

0.459

  pilA2 Legionella pneumophila str. Paris

87.097

50.82

0.443

  pilA2 Legionella pneumophila strain ERS1305867

87.097

50.82

0.443

  pilA/pilA1 Eikenella corrodens VA1

77.143

57.377

0.443

  pilE Acinetobacter baumannii D1279779

56.522

75.41

0.426

  comF Acinetobacter baylyi ADP1

57.778

73.77

0.426

  pilA/pilAI Pseudomonas stutzeri DSM 10701

78.788

54.098

0.426

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

75.758

54.098

0.41

  pilL Neisseria gonorrhoeae MS11

69.444

59.016

0.41

  pilX Neisseria meningitidis 8013

69.444

59.016

0.41

  pilA Haemophilus influenzae Rd KW20

72.727

54.098

0.393

  pilE Neisseria gonorrhoeae strain FA1090

72.727

54.098

0.393

  pilE Neisseria gonorrhoeae MS11

72.727

54.098

0.393

  pilA Haemophilus influenzae 86-028NP

72.727

54.098

0.393

  pilH Neisseria gonorrhoeae MS11

44.898

80.328

0.361

  pilA Glaesserella parasuis strain SC1401

68.75

52.459

0.361

  comE Acinetobacter baylyi ADP1

68.75

52.459

0.361