Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   M0M42_RS16505 Genome accession   NZ_CP096208
Coordinates   3533864..3535570 (-) Length   568 a.a.
NCBI ID   WP_248493407.1    Uniprot ID   -
Organism   Pseudomonas knackmussii strain B09-2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3528864..3540570
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M0M42_RS16480 (M0M42_16480) - 3529759..3530451 (+) 693 WP_248493399.1 energy-coupling factor ABC transporter permease -
  M0M42_RS16485 (M0M42_16485) yacG 3530501..3530698 (-) 198 WP_248493401.1 DNA gyrase inhibitor YacG -
  M0M42_RS16490 (M0M42_16490) coaE 3530695..3531303 (-) 609 WP_248497435.1 dephospho-CoA kinase -
  M0M42_RS16495 (M0M42_16495) pilD 3531772..3532635 (-) 864 WP_248493403.1 A24 family peptidase Machinery gene
  M0M42_RS16500 (M0M42_16500) pilC 3532638..3533861 (-) 1224 WP_248493405.1 type II secretion system F family protein Machinery gene
  M0M42_RS16505 (M0M42_16505) pilB 3533864..3535570 (-) 1707 WP_248493407.1 type IV-A pilus assembly ATPase PilB Machinery gene
  M0M42_RS21130 pilA 3536043..3536228 (+) 186 WP_282579612.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  M0M42_RS16515 (M0M42_16515) - 3536241..3536654 (+) 414 WP_248493410.1 hypothetical protein -
  M0M42_RS16520 (M0M42_16520) - 3536724..3537527 (+) 804 WP_248493412.1 ABC transporter permease subunit -
  M0M42_RS16525 (M0M42_16525) - 3537524..3538357 (+) 834 WP_248493414.1 hypothetical protein -
  M0M42_RS16530 (M0M42_16530) - 3538344..3539231 (+) 888 WP_248493416.1 ABC transporter ATP-binding protein -
  M0M42_RS16535 (M0M42_16535) - 3539838..3540278 (+) 441 WP_248493417.1 YtoQ family protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62651.81 Da        Isoelectric Point: 5.6141

>NTDB_id=680424 M0M42_RS16505 WP_248493407.1 3533864..3535570(-) (pilB) [Pseudomonas knackmussii strain B09-2]
MNENAPLGGLARQLVLVGQLDEKIALQAQQQAQRNQVPLVSWLVQNKLLKSRAIVDVAGEQFGVPYFDLSALDKEAQPKD
LVSEKLVRQHRVLPLQRRGNKLYVAVSDPTNHQAISDIQFNTGLSVESVLVEDDKLGEAIEKLFESANTALDGLTDSDLD
DLDIEMGGGAVEKEEGEHNEADDAPVVRFVNKMLLDAIKRGSSDLHFEPYEKSYRVRFRTDGVLHEVARPPVQLVSRISA
RLKVMAALDIAERRKPQDGRIKMRVSKTKSIDFRVNSLPTLWGEKIVMRILDSSSAKMGIDALGYEEDQKELYMTALKQP
QGMILVTGPTGSGKTVSLYTGLNILNTIDVNISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDIIMVGEIR
DLETAEIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCASCKKEVEVPREALLKEGI
PENKIGTFQLYGPVGCENCNGGYKGRVGIYEVVKITPALQRLIMEEGNSIDIAIQARKDGFNDLRLSGLVKAMHGITSLE
EVNRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=680424 M0M42_RS16505 WP_248493407.1 3533864..3535570(-) (pilB) [Pseudomonas knackmussii strain B09-2]
ATGAACGAAAACGCTCCCCTCGGCGGTCTGGCGCGCCAGTTGGTATTGGTCGGCCAGCTTGATGAGAAAATTGCGCTACA
GGCGCAACAACAGGCCCAGCGTAATCAGGTACCGCTGGTTTCCTGGCTGGTGCAGAACAAACTGTTAAAAAGCCGAGCGA
TAGTCGATGTCGCGGGAGAGCAGTTTGGGGTTCCGTATTTCGACCTGAGTGCGCTGGACAAGGAAGCCCAGCCTAAAGAC
CTGGTTAGCGAAAAGCTGGTACGCCAGCATCGTGTGCTTCCGCTGCAGCGGCGCGGTAATAAGCTGTATGTCGCCGTTTC
CGATCCAACCAATCACCAAGCCATCAGCGATATCCAGTTCAATACCGGGCTGTCGGTCGAGTCGGTGCTGGTTGAAGATG
ACAAGCTTGGCGAGGCCATTGAAAAGCTATTCGAATCTGCCAATACGGCGCTGGACGGACTGACCGATTCCGATCTGGAT
GATCTGGATATCGAGATGGGTGGCGGCGCCGTCGAGAAAGAGGAGGGCGAGCACAACGAGGCTGACGATGCGCCGGTGGT
GCGCTTCGTCAACAAGATGCTGCTCGATGCGATCAAGCGCGGCTCATCGGACTTGCACTTCGAACCCTACGAGAAATCCT
ATCGGGTGCGTTTTCGTACCGATGGCGTGTTGCATGAAGTGGCAAGGCCGCCGGTGCAGCTGGTCAGTCGAATCTCGGCA
CGTCTCAAGGTGATGGCGGCGCTGGACATTGCCGAACGACGCAAGCCGCAAGACGGCCGAATAAAAATGAGGGTTTCAAA
GACCAAGTCCATCGATTTTCGCGTCAACAGCCTCCCCACGCTTTGGGGCGAAAAGATCGTGATGCGTATCCTCGATTCGT
CCAGCGCCAAAATGGGCATTGATGCACTTGGCTACGAGGAAGATCAAAAAGAGTTGTACATGACGGCGCTAAAGCAGCCG
CAAGGAATGATCTTGGTCACCGGCCCAACCGGCTCGGGCAAAACGGTCTCCCTGTATACCGGCCTAAACATTCTCAATAC
GATCGATGTGAACATCTCCACTGCCGAAGACCCGGTTGAGATCAACCTTGAAGGTATTAACCAGGTCAACGTCAATCCAC
GCCAGGGGATGGACTTCGCCCAGGCGCTGCGCGCCTTCCTGCGCCAGGACCCTGACATCATCATGGTGGGCGAGATTCGC
GACCTGGAAACCGCTGAGATTGCCGTTAAGGCCGCCCAGACCGGGCATATGGTGATGTCGACGCTGCACACTAACAGCGC
TGCGGAAACCCTGACTCGCCTACGTAACATGGGAGTGCCGGCGTTCAATATCGCGACTTCGGTCAACCTGATCATCGCCC
AGCGTCTAGCTCGCAAGCTCTGCGCGAGTTGTAAGAAGGAAGTGGAGGTGCCCCGCGAAGCACTTCTGAAGGAAGGTATT
CCGGAAAACAAGATAGGGACCTTTCAGCTCTACGGACCGGTGGGTTGTGAGAATTGCAACGGCGGGTACAAGGGCCGTGT
CGGTATTTATGAAGTGGTTAAAATCACGCCTGCTCTGCAGCGGCTTATCATGGAGGAAGGAAACTCCATCGATATCGCCA
TCCAAGCGCGGAAAGATGGCTTCAATGATTTGCGTCTTTCGGGTCTGGTCAAAGCCATGCACGGCATCACCAGCCTGGAG
GAAGTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.596

100

0.588
  pilB Acinetobacter baylyi ADP1

57.522

99.472

0.572
  pilB Legionella pneumophila strain ERS1305867

53.846

100

0.542
  pilB Vibrio cholerae strain A1552

51.064

99.296

0.507
  pilB Vibrio parahaemolyticus RIMD 2210633

50.556

95.07

0.481
  pilF Neisseria gonorrhoeae MS11

52.93

90.141

0.477
  pilB Vibrio campbellii strain DS40M4

50.279

94.542

0.475
  pilF Thermus thermophilus HB27

38.732

100

0.387
  pilB/pilB1 Synechocystis sp. PCC 6803

40.115

91.725

0.368