Detailed information NTG0465945

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: HU825_RS18760
• Genome accession: NZ_CP058908
• Coordinates: 1941844..1942026 (-)
• Length: 60 a.a.
• NCBI ID: WP_284692207.1
• Uniprot ID: -
• Organism: Pseudomonas phenolilytica strain RBPA9
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1936844..1947026

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HU825_RS09285 (HU825_09275)	-	1938485..1939369 (-)	885	WP_234303356.1	ABC transporter ATP-binding protein	-
HU825_RS09290 (HU825_09280)	-	1939384..1940214 (-)	831	WP_054093587.1	hypothetical protein	-
HU825_RS09295 (HU825_09285)	-	1940216..1941025 (-)	810	WP_054093588.1	ABC transporter permease	-
HU825_RS09300 (HU825_09290)	-	1941102..1941281 (-)	180	WP_081002739.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	-
HU825_RS09305 (HU825_09295)	-	1941391..1941831 (-)	441	WP_152947535.1	hypothetical protein	-
HU825_RS18760 (HU825_09300)	pilA	1941844..1942026 (-)	183	WP_284692207.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
HU825_RS09315 (HU825_09305)	pilB	1942401..1944104 (+)	1704	WP_234303357.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HU825_RS09320 (HU825_09310)	pilC	1944107..1945327 (+)	1221	WP_043295815.1	type II secretion system F family protein	Machinery gene
HU825_RS09325 (HU825_09315)	pilD	1945331..1946197 (+)	867	WP_284692208.1	A24 family peptidase	Machinery gene
HU825_RS09330 (HU825_09320)	coaE	1946300..1946905 (+)	606	WP_043295817.1	dephospho-CoA kinase	-

Sequence

Protein

• Length: 60 a.a.
• Molecular weight: 6390.59 Da
• Isoelectric Point: 10.2806

>NTDB_id=465945 HU825_RS18760 WP_284692207.1 1941844..1942026(-) (pilA) [Pseudomonas phenolilytica strain RBPA9]
MKSKKQKGFTLIELMIVVAIIGILAAIAIPQFNEYRAKSNDTVATADAKNSISVLAAAQL

Nucleotide

• Length: 183 bp

>NTDB_id=465945 HU825_RS18760 WP_284692207.1 1941844..1942026(-) (pilA) [Pseudomonas phenolilytica strain RBPA9]
ATGAAGTCCAAGAAGCAAAAAGGTTTCACCCTGATTGAATTGATGATCGTAGTTGCAATCATCGGCATTCTGGCTGCTAT
CGCTATTCCGCAGTTCAACGAATACCGCGCAAAATCCAATGACACTGTCGCAACGGCCGATGCAAAAAATTCCATCAGCG
TTTTAGCAGCCGCCCAGCTTTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (4-29)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Pseudomonas aeruginosa PAK	74.419	71.667	0.533
pilA	Vibrio parahaemolyticus RIMD 2210633	62.5	80	0.5
pilA	Acinetobacter baumannii strain A118	88.235	56.667	0.5
pilA2	Legionella pneumophila strain ERS1305867	65.217	76.667	0.5
pilA2	Legionella pneumophila str. Paris	65.217	76.667	0.5
pilV	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	49.153	98.333	0.483
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	76.316	63.333	0.483
pilA	Vibrio cholerae strain A1552	76.316	63.333	0.483
pilA	Vibrio cholerae C6706	76.316	63.333	0.483
pilA/pilAI	Pseudomonas stutzeri DSM 10701	72.5	66.667	0.483
pilA	Acinetobacter nosocomialis M2	85.294	56.667	0.483
pilA/pilA1	Eikenella corrodens VA1	66.667	70	0.467
pilA	Vibrio campbellii strain DS40M4	73.684	63.333	0.467
pilA/pilA1	Synechocystis sp. PCC 6803	44.828	96.667	0.433
pilA	Ralstonia pseudosolanacearum GMI1000	61.905	70	0.433
pilA/pilA4	Thermus thermophilus HB27	60.465	71.667	0.433
pilA/pilAII	Pseudomonas stutzeri DSM 10701	60.976	68.333	0.417
comE	Acinetobacter baylyi ADP1	62.5	66.667	0.417
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	73.529	56.667	0.417
pilY2	Acinetobacter baumannii D1279779	46.296	90	0.417
comF	Acinetobacter baylyi ADP1	68.571	58.333	0.4
pilA	Haemophilus influenzae 86-028NP	67.647	56.667	0.383
pilE	Neisseria gonorrhoeae MS11	67.647	56.667	0.383
pilE	Neisseria gonorrhoeae strain FA1090	67.647	56.667	0.383
pilA	Haemophilus influenzae Rd KW20	67.647	56.667	0.383
pilA	Glaesserella parasuis strain SC1401	68.75	53.333	0.367
pilL	Neisseria gonorrhoeae MS11	62.857	58.333	0.367