Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   HU825_RS09315 Genome accession   NZ_CP058908
Coordinates   1942401..1944104 (+) Length   567 a.a.
NCBI ID   WP_234303357.1    Uniprot ID   -
Organism   Pseudomonas phenolilytica strain RBPA9     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1937401..1949104
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU825_RS09285 (HU825_09275) - 1938485..1939369 (-) 885 WP_234303356.1 ABC transporter ATP-binding protein -
  HU825_RS09290 (HU825_09280) - 1939384..1940214 (-) 831 WP_054093587.1 hypothetical protein -
  HU825_RS09295 (HU825_09285) - 1940216..1941025 (-) 810 WP_054093588.1 ABC transporter permease -
  HU825_RS09300 (HU825_09290) - 1941102..1941281 (-) 180 WP_081002739.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  HU825_RS09305 (HU825_09295) - 1941391..1941831 (-) 441 WP_152947535.1 hypothetical protein -
  HU825_RS18760 (HU825_09300) pilA 1941844..1942026 (-) 183 WP_284692207.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HU825_RS09315 (HU825_09305) pilB 1942401..1944104 (+) 1704 WP_234303357.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HU825_RS09320 (HU825_09310) pilC 1944107..1945327 (+) 1221 WP_043295815.1 type II secretion system F family protein Machinery gene
  HU825_RS09325 (HU825_09315) pilD 1945331..1946197 (+) 867 WP_284692208.1 A24 family peptidase Machinery gene
  HU825_RS09330 (HU825_09320) coaE 1946300..1946905 (+) 606 WP_043295817.1 dephospho-CoA kinase -
  HU825_RS09335 (HU825_09325) yacG 1946902..1947099 (+) 198 WP_223250045.1 DNA gyrase inhibitor YacG -
  HU825_RS09340 (HU825_09330) - 1947137..1947820 (-) 684 WP_234303358.1 energy-coupling factor ABC transporter permease -
  HU825_RS09345 (HU825_09335) rplM 1948193..1948621 (+) 429 WP_043295819.1 50S ribosomal protein L13 -
  HU825_RS09350 (HU825_09340) rpsI 1948636..1949028 (+) 393 WP_043295820.1 30S ribosomal protein S9 -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62291.33 Da        Isoelectric Point: 5.7757

>NTDB_id=465931 HU825_RS09315 WP_234303357.1 1942401..1944104(+) (pilB) [Pseudomonas phenolilytica strain RBPA9]
MNETVSLGGLARQLVLAGQLDEKAAMQAQLQAQRNQSSLVTWLVQNKLVKSRVLGEIAADQFGVPFFDLSALDKDTQPRD
LVSEKLVRQHRALPLQRRGNKLYVAVSDPTNHQAISDIQFNTGLSVEGVLVEDDKLGEAIEKFFESAATALDGLSSSDLE
DLDVEVAGEAKDDGAEHSEADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKTYRVRFRTDGMLHEVARPPVQLVSRISAR
LKVMASLDIAERRKPQDGRIKMKVSKTKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDALGYEDDQKELYMNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPACKREVDVPREVLLKEGFP
VEKIGTFKLFGPVGCDSCKGGYKGRVGIYEVVKITPALQRLIMEEGNSIDIAQQARKDGFNDLRTSGLVKAMHGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=465931 HU825_RS09315 WP_234303357.1 1942401..1944104(+) (pilB) [Pseudomonas phenolilytica strain RBPA9]
ATGAACGAAACTGTTTCCCTCGGTGGTCTCGCGCGGCAGTTGGTGCTGGCGGGCCAGTTGGATGAAAAGGCTGCGATGCA
GGCGCAGCTGCAGGCACAGCGCAATCAGTCGTCGCTTGTGACGTGGCTGGTACAGAACAAGCTGGTCAAGAGTCGTGTGC
TTGGGGAGATCGCCGCCGACCAGTTCGGCGTGCCGTTCTTTGATCTCTCGGCGCTAGATAAGGATACCCAGCCGCGCGAT
CTGGTCAGCGAAAAGCTGGTGCGCCAGCACCGTGCTCTACCGTTGCAACGCCGTGGCAATAAGCTGTACGTGGCAGTGTC
GGACCCGACTAATCATCAGGCCATCAGCGATATCCAGTTCAATACCGGACTCTCTGTCGAGGGCGTGCTGGTCGAGGATG
ACAAGCTGGGCGAGGCCATCGAAAAGTTCTTCGAGTCGGCCGCTACGGCACTCGACGGTTTGAGCAGCTCGGATCTGGAG
GATCTGGATGTTGAGGTGGCCGGAGAGGCGAAAGATGATGGCGCGGAGCACAGCGAGGCCGATGACGCGCCGGTGGTGCG
CTTCGTCAATAAGATGTTGTTGGATGCCATCAAGGGTGGCTCGTCCGACCTGCACTTCGAGCCTTACGAGAAGACCTATC
GCGTGCGCTTTCGTACCGACGGCATGTTGCACGAGGTTGCTCGCCCTCCAGTGCAGTTGGTCAGCAGGATTTCGGCGCGA
TTGAAGGTCATGGCCAGCCTGGATATCGCCGAGCGGCGTAAGCCGCAGGACGGGCGAATCAAGATGAAGGTGTCAAAAAC
CAAGTCCATTGATTTTCGCGTGAACACGCTGCCCACGCTGTGGGGCGAGAAGATCGTGATGCGGATTCTGGATTCGGCCA
GCGCGCAGATGGGGATCGATGCCCTGGGTTATGAGGACGACCAGAAAGAGCTGTACATGAACGCCCTTAAACAGCCGCAG
GGCATGATCCTGGTGACTGGCCCCACGGGATCGGGCAAGACGGTTTCGTTGTATACCGGCCTGAACATCCTCAATACCGT
TGACGTGAACATCTCTACCGCGGAGGACCCGGTGGAGATCAACCTGGAGGGGATCAACCAGGTCAACGTCAATCCGCGTC
AGGGAATGGATTTTGCTCAGGCGTTGCGTGCTTTTCTGCGCCAAGACCCAGATATCATCATGGTCGGCGAGATTCGTGAC
CTTGAAACCGCCGAGATTGCTATCAAGGCGGCGCAAACCGGGCATATGGTCATGTCGACGCTGCACACCAATAGCGCAGC
GGAAACGCTGACGCGTCTGCGTAATATGGGGGTGCCGGCTTTCAATATTGCGACTTCGGTCAACTTGATCATTGCGCAGC
GGTTGGCGCGCAAGCTTTGCCCGGCGTGCAAGAGGGAGGTTGACGTGCCGCGCGAGGTGCTTCTCAAGGAGGGGTTCCCG
GTTGAAAAGATCGGCACATTCAAACTATTTGGTCCAGTGGGCTGCGATAGTTGCAAGGGTGGCTACAAGGGGCGTGTCGG
TATTTATGAAGTGGTTAAAATCACGCCAGCGTTGCAGCGGCTTATCATGGAAGAAGGTAACTCCATCGATATCGCCCAGC
AGGCGCGCAAAGATGGTTTCAATGATTTGCGCACTTCGGGACTGGTCAAGGCCATGCACGGCGTCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.091

100

0.596
  pilB Acinetobacter baylyi ADP1

59.115

99.647

0.589
  pilB Legionella pneumophila strain ERS1305867

54.72

100

0.552
  pilB Vibrio cholerae strain A1552

50.444

99.295

0.501
  pilF Neisseria gonorrhoeae MS11

53.816

90.123

0.485
  pilB Vibrio parahaemolyticus RIMD 2210633

51.509

93.474

0.481
  pilB Vibrio campbellii strain DS40M4

51.224

93.651

0.48
  pilB/pilB1 Synechocystis sp. PCC 6803

36.825

100

0.397
  pilF Thermus thermophilus HB27

37.722

99.118

0.374
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.205

93.122

0.365