Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HU825_RS09320 Genome accession   NZ_CP058908
Coordinates   1944107..1945327 (+) Length   406 a.a.
NCBI ID   WP_043295815.1    Uniprot ID   -
Organism   Pseudomonas phenolilytica strain RBPA9     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 1945331..1956556 1944107..1945327 flank 4


Gene organization within MGE regions


Location: 1944107..1956556
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HU825_RS09320 (HU825_09310) pilC 1944107..1945327 (+) 1221 WP_043295815.1 type II secretion system F family protein Machinery gene
  HU825_RS09325 (HU825_09315) pilD 1945331..1946197 (+) 867 WP_284692208.1 A24 family peptidase Machinery gene
  HU825_RS09330 (HU825_09320) coaE 1946300..1946905 (+) 606 WP_043295817.1 dephospho-CoA kinase -
  HU825_RS09335 (HU825_09325) yacG 1946902..1947099 (+) 198 WP_223250045.1 DNA gyrase inhibitor YacG -
  HU825_RS09340 (HU825_09330) - 1947137..1947820 (-) 684 WP_234303358.1 energy-coupling factor ABC transporter permease -
  HU825_RS09345 (HU825_09335) rplM 1948193..1948621 (+) 429 WP_043295819.1 50S ribosomal protein L13 -
  HU825_RS09350 (HU825_09340) rpsI 1948636..1949028 (+) 393 WP_043295820.1 30S ribosomal protein S9 -
  HU825_RS09355 (HU825_09345) petA 1949280..1949873 (+) 594 WP_043295821.1 ubiquinol-cytochrome c reductase iron-sulfur subunit -
  HU825_RS09360 (HU825_09350) - 1949873..1951084 (+) 1212 WP_043295822.1 cytochrome bc complex cytochrome b subunit -
  HU825_RS09365 (HU825_09355) - 1951084..1951863 (+) 780 WP_043295823.1 cytochrome c1 -
  HU825_RS09370 (HU825_09360) - 1951951..1952568 (+) 618 WP_043295824.1 glutathione S-transferase N-terminal domain-containing protein -
  HU825_RS09375 (HU825_09365) - 1952583..1952990 (+) 408 WP_234303359.1 ClpXP protease specificity-enhancing factor -
  HU825_RS09380 (HU825_09370) - 1953136..1953711 (-) 576 WP_043295826.1 BON domain-containing protein -
  HU825_RS09385 (HU825_09375) - 1953708..1954301 (-) 594 WP_043295827.1 phosphoheptose isomerase -
  HU825_RS09390 (HU825_09380) - 1954380..1954742 (-) 363 WP_043295828.1 YraN family protein -
  HU825_RS09395 (HU825_09385) - 1954739..1956556 (-) 1818 WP_081002740.1 penicillin-binding protein activator -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44509.44 Da        Isoelectric Point: 9.7515

>NTDB_id=465932 HU825_RS09320 WP_043295815.1 1944107..1945327(+) (pilC) [Pseudomonas phenolilytica strain RBPA9]
MAEKALKTSVFTWEGTDRKGGKVKGELTGQSPALIKAQLRKQGINPLKVRKKAVSLFGSGKKIKPMDIALFTRQMATMMK
AGVPLLQSFDIIGEGFDNPNMRKLVDEIKQEVAAGNSFAGSLRKKPQYFDDLYCNLVESGEQSGALETLLDRVATYKEKT
EALKAKIKKAMTYPIAVIVVAIIVSAILLIKVVPQFESVFEGFGAQLPAFTMMVVYLSRWLQEWWYVVFAVMFAAAFVFK
HVYKSSEKFRDSLDRLLLKLPIVGDILYKSVVARYARTLSTTFAAGVPLVDALDSVAGATGNVVFRNAVAKIRSDVSSGV
QLNFSMRTTGIFPTMAIQMTAIGEESGALDEMLDKLASFYEAEVDNMVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIFQ
LGSVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=465932 HU825_RS09320 WP_043295815.1 1944107..1945327(+) (pilC) [Pseudomonas phenolilytica strain RBPA9]
ATGGCGGAAAAAGCGTTGAAAACCAGTGTGTTCACCTGGGAGGGCACCGATAGAAAAGGTGGCAAGGTCAAAGGTGAGTT
GACTGGTCAAAGTCCGGCGCTCATCAAGGCGCAGCTTCGCAAGCAGGGCATCAATCCGCTAAAGGTCCGCAAGAAAGCCG
TTTCGCTGTTCGGCTCGGGTAAGAAGATCAAGCCGATGGATATCGCGCTGTTCACCCGGCAGATGGCGACGATGATGAAG
GCGGGGGTTCCACTTTTGCAGTCGTTCGACATTATCGGCGAGGGTTTCGACAATCCCAACATGCGCAAGCTGGTGGACGA
GATCAAACAGGAAGTGGCCGCGGGTAACAGTTTCGCCGGTTCGCTGCGCAAGAAGCCGCAATATTTCGACGATCTGTATT
GCAATCTGGTCGAGTCTGGCGAGCAGTCCGGTGCGCTGGAAACGCTTCTGGATCGCGTAGCGACCTACAAGGAAAAAACC
GAAGCGCTCAAGGCGAAGATAAAGAAGGCCATGACCTACCCGATCGCGGTGATCGTGGTGGCGATCATCGTTTCGGCGAT
TCTGCTCATTAAGGTCGTTCCACAGTTCGAGAGCGTATTCGAAGGGTTTGGCGCCCAACTACCGGCTTTCACCATGATGG
TGGTCTACCTTTCGCGTTGGTTGCAGGAGTGGTGGTACGTAGTATTCGCGGTTATGTTTGCCGCAGCATTCGTATTCAAG
CATGTCTACAAGTCATCGGAAAAATTTCGCGACTCGCTCGATCGGCTGCTGCTTAAGCTGCCGATCGTTGGAGATATTCT
CTACAAGTCGGTAGTGGCGCGTTACGCGCGAACACTGTCTACCACGTTCGCGGCGGGTGTACCGCTGGTGGATGCGCTGG
ATTCGGTCGCCGGCGCCACGGGTAACGTGGTGTTTCGCAATGCGGTGGCGAAGATCCGTAGCGATGTTTCGTCGGGCGTT
CAGCTCAATTTCTCCATGCGTACCACTGGGATCTTCCCAACGATGGCCATTCAGATGACGGCAATCGGCGAGGAGTCCGG
CGCGCTGGATGAAATGCTGGATAAGCTGGCGAGCTTCTACGAGGCCGAGGTCGATAACATGGTGGACAACCTGACCACCC
TGATGGAGCCGATGATTATGGCTGTCCTGGGTGTGTTGGTTGGGGGGTTGATCATTGCGATGTACTTGCCGATCTTCCAG
TTGGGCAGCGTGGTCGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

82.469

99.754

0.823
  pilC Acinetobacter baylyi ADP1

63.054

100

0.631
  pilC Acinetobacter baumannii D1279779

61.481

99.754

0.613
  pilC Legionella pneumophila strain ERS1305867

53.535

97.537

0.522
  pilG Neisseria gonorrhoeae MS11

45.161

99.261

0.448
  pilG Neisseria meningitidis 44/76-A

45.161

99.261

0.448
  pilC Vibrio cholerae strain A1552

42.857

100

0.429
  pilC Vibrio campbellii strain DS40M4

40.05

97.783

0.392