Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilV   Type   Machinery gene
Locus tag   HNE05_RS20545 Genome accession   NZ_CP053697
Coordinates   960788..960973 (+) Length   61 a.a.
NCBI ID   WP_173203713.1    Uniprot ID   A0A6M8FDY9
Organism   Aquipseudomonas campi strain S1-A32-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 953016..964141 960788..960973 within 0


Gene organization within MGE regions


Location: 953016..964141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNE05_RS04325 (HNE05_01935) - 953016..953483 (+) 468 WP_173203699.1 FAD/FMN-containing dehydrogenase -
  HNE05_RS04330 (HNE05_01940) - 953480..954169 (+) 690 WP_173203701.1 energy-coupling factor ABC transporter permease -
  HNE05_RS04335 (HNE05_01945) - 954273..954488 (+) 216 WP_173211596.1 hypothetical protein -
  HNE05_RS04340 (HNE05_01950) - 954631..955771 (+) 1141 Protein_853 IS481 family transposase -
  HNE05_RS04345 (HNE05_01955) yacG 955945..956139 (-) 195 WP_173203703.1 DNA gyrase inhibitor YacG -
  HNE05_RS04350 (HNE05_01960) coaE 956136..956747 (-) 612 WP_173203705.1 dephospho-CoA kinase -
  HNE05_RS04355 (HNE05_01965) pilD 956748..957614 (-) 867 WP_173203707.1 prepilin peptidase Machinery gene
  HNE05_RS04360 (HNE05_01970) pilC 957618..958838 (-) 1221 WP_173203709.1 type II secretion system F family protein Machinery gene
  HNE05_RS04365 (HNE05_01975) pilB 958842..960551 (-) 1710 WP_173203711.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HNE05_RS20545 (HNE05_01980) pilV 960788..960973 (+) 186 WP_173203713.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HNE05_RS04375 (HNE05_01985) - 960990..961445 (+) 456 WP_173203716.1 hypothetical protein -
  HNE05_RS04380 (HNE05_01990) - 961591..962379 (+) 789 WP_240008815.1 hypothetical protein -
  HNE05_RS04385 (HNE05_01995) - 962382..963221 (+) 840 WP_173203718.1 hypothetical protein -
  HNE05_RS04390 (HNE05_02000) - 963221..964141 (+) 921 WP_173203720.1 ABC transporter ATP-binding protein -

Sequence


Protein


Download         Length: 61 a.a.        Molecular weight: 6562.73 Da        Isoelectric Point: 9.9318

>NTDB_id=446405 HNE05_RS20545 WP_173203713.1 960788..960973(+) (pilV) [Aquipseudomonas campi strain S1-A32-2]
MKSLKQQKGFTLIELMIVIAIIGILAAIAIPQFNEYRAKANDSTALADAKNNTTAIVSSLQ

Nucleotide


Download         Length: 186 bp        

>NTDB_id=446405 HNE05_RS20545 WP_173203713.1 960788..960973(+) (pilV) [Aquipseudomonas campi strain S1-A32-2]
ATGAAGTCTCTCAAGCAACAAAAAGGCTTTACCCTGATCGAGCTGATGATCGTAATCGCGATCATCGGTATTCTCGCCGC
AATTGCTATTCCGCAGTTCAATGAATATCGTGCTAAGGCGAATGATTCAACTGCCTTGGCCGATGCTAAGAATAACACTA
CGGCTATCGTATCCTCGCTTCAATAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A6M8FDY9

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter nosocomialis M2

58.621

95.082

0.557

  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

58.929

91.803

0.541

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

54.098

100

0.541

  pilA/pilAII Pseudomonas stutzeri DSM 10701

43.836

100

0.525

  comP Acinetobacter baylyi ADP1

58.491

86.885

0.508

  pilA Pseudomonas aeruginosa PAK

70.455

72.131

0.508

  pilA Ralstonia pseudosolanacearum GMI1000

57.407

88.525

0.508

  pilA2 Legionella pneumophila strain ERS1305867

65.217

75.41

0.492

  pilA2 Legionella pneumophila str. Paris

65.217

75.41

0.492

  pilA Vibrio campbellii strain DS40M4

69.048

68.852

0.475

  pilA Haemophilus influenzae 86-028NP

67.442

70.492

0.475

  pilE Neisseria gonorrhoeae MS11

58

81.967

0.475

  pilE Neisseria gonorrhoeae strain FA1090

58

81.967

0.475

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

65.909

72.131

0.475

  pilA Vibrio cholerae C6706

65.909

72.131

0.475

  pilA Vibrio cholerae strain A1552

65.909

72.131

0.475

  pilA/pilAI Pseudomonas stutzeri DSM 10701

66.667

68.852

0.459

  pilA/pilA1 Eikenella corrodens VA1

77.778

59.016

0.459

  pilA Acinetobacter baumannii strain A118

62.222

73.77

0.459

  pilA Vibrio parahaemolyticus RIMD 2210633

56.522

75.41

0.426

  pilA/pilA4 Thermus thermophilus HB27

56.818

72.131

0.41

  pilA Haemophilus influenzae Rd KW20

73.529

55.738

0.41

  comF Acinetobacter baylyi ADP1

60

65.574

0.393

  pilA Glaesserella parasuis strain SC1401

72.727

54.098

0.393

  pilX Neisseria meningitidis 8013

57.895

62.295

0.361

  pilL Neisseria gonorrhoeae MS11

57.895

62.295

0.361

  pilY2 Acinetobacter baumannii D1279779

48.889

73.77

0.361