Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   HNE05_RS04360 Genome accession   NZ_CP053697
Coordinates   957618..958838 (-) Length   406 a.a.
NCBI ID   WP_173203709.1    Uniprot ID   A0A6M8FBD8
Organism   Aquipseudomonas campi strain S1-A32-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 953016..964141 957618..958838 within 0


Gene organization within MGE regions


Location: 953016..964141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  HNE05_RS04325 (HNE05_01935) - 953016..953483 (+) 468 WP_173203699.1 FAD/FMN-containing dehydrogenase -
  HNE05_RS04330 (HNE05_01940) - 953480..954169 (+) 690 WP_173203701.1 energy-coupling factor ABC transporter permease -
  HNE05_RS04335 (HNE05_01945) - 954273..954488 (+) 216 WP_173211596.1 hypothetical protein -
  HNE05_RS04340 (HNE05_01950) - 954631..955771 (+) 1141 Protein_853 IS481 family transposase -
  HNE05_RS04345 (HNE05_01955) yacG 955945..956139 (-) 195 WP_173203703.1 DNA gyrase inhibitor YacG -
  HNE05_RS04350 (HNE05_01960) coaE 956136..956747 (-) 612 WP_173203705.1 dephospho-CoA kinase -
  HNE05_RS04355 (HNE05_01965) pilD 956748..957614 (-) 867 WP_173203707.1 prepilin peptidase Machinery gene
  HNE05_RS04360 (HNE05_01970) pilC 957618..958838 (-) 1221 WP_173203709.1 type II secretion system F family protein Machinery gene
  HNE05_RS04365 (HNE05_01975) pilB 958842..960551 (-) 1710 WP_173203711.1 type IV-A pilus assembly ATPase PilB Machinery gene
  HNE05_RS20545 (HNE05_01980) pilV 960788..960973 (+) 186 WP_173203713.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  HNE05_RS04375 (HNE05_01985) - 960990..961445 (+) 456 WP_173203716.1 hypothetical protein -
  HNE05_RS04380 (HNE05_01990) - 961591..962379 (+) 789 WP_240008815.1 hypothetical protein -
  HNE05_RS04385 (HNE05_01995) - 962382..963221 (+) 840 WP_173203718.1 hypothetical protein -
  HNE05_RS04390 (HNE05_02000) - 963221..964141 (+) 921 WP_173203720.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44304.15 Da        Isoelectric Point: 9.9508

>NTDB_id=446380 HNE05_RS04360 WP_173203709.1 957618..958838(-) (pilC) [Aquipseudomonas campi strain S1-A32-2]
MAAKTLKTSAFIWEGTDKKGAKIKGELIGESPALIKAQLRKQGINPLKVRKKPASLFSAGKKIKPLDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENPNMRKLVDEVKQEVAAGNSFAASLRKKPLFFDDLYCNLVDSGEQSGALETLLDRIATYKEKT
EALKAKIKKAMTYPIAVIIVAVIVTAVLLIKVVPQFESVFKGFGAELPAFTQVVVNMSRGLQEWWFIFLFAIFGAIFAFK
TTHQRSEKFRDFLDRTLLKAPIVGDILYKAVVARYARTLATTFAAGVPLVDALDSVAGATGNVVFRNAVHKIRNDVSSGT
QLNFSMRTTGIFPTMAIQMTAIGEESGSLDEMLEKVASFYEAEVDNAVDNLTTLMEPLIMSVIGVLVGGLIVAMYLPIFQ
LGAVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=446380 HNE05_RS04360 WP_173203709.1 957618..958838(-) (pilC) [Aquipseudomonas campi strain S1-A32-2]
ATGGCGGCCAAAACTCTAAAAACCAGTGCCTTTATCTGGGAAGGCACCGACAAAAAAGGCGCCAAGATCAAGGGCGAGCT
AATCGGCGAATCACCAGCCCTGATCAAAGCTCAATTGCGCAAGCAGGGCATCAACCCGCTGAAAGTCCGCAAAAAGCCGG
CCTCTCTCTTTAGCGCTGGCAAGAAAATAAAGCCACTGGATATCGCCCTGTTTACCCGGCAGATGGCCACCATGATGAAA
GCAGGCGTGCCATTGCTGCAGTCCTTCGACATCATCGGTGAGGGCTTCGAAAACCCCAATATGCGCAAGCTGGTCGACGA
AGTGAAGCAGGAAGTAGCAGCCGGCAACAGCTTCGCGGCCTCGCTGCGCAAGAAGCCGCTGTTCTTTGATGACCTGTATT
GCAACCTGGTGGACTCGGGCGAACAGTCTGGTGCCCTTGAAACCCTGCTGGATCGAATCGCAACCTATAAAGAGAAGACC
GAGGCGCTAAAGGCCAAGATCAAAAAGGCCATGACCTACCCTATTGCGGTAATCATCGTTGCAGTTATCGTAACTGCCGT
CCTACTGATCAAGGTGGTTCCGCAATTTGAGAGCGTATTCAAAGGCTTTGGTGCCGAACTACCTGCATTCACTCAGGTAG
TCGTTAATATGTCACGGGGCCTGCAGGAGTGGTGGTTCATTTTTCTGTTCGCCATTTTCGGCGCCATCTTCGCCTTCAAA
ACGACCCACCAGCGCTCCGAGAAATTTCGCGACTTTCTCGACCGCACCCTACTCAAGGCGCCAATTGTCGGCGACATCCT
CTACAAAGCCGTAGTGGCTCGCTATGCCCGCACATTGGCAACCACCTTTGCTGCCGGCGTACCACTGGTGGACGCACTCG
ACTCAGTCGCCGGCGCCACGGGCAACGTGGTCTTCCGCAATGCTGTGCATAAAATCCGCAACGACGTCTCCTCAGGTACC
CAGCTGAACTTCTCCATGCGTACTACCGGCATTTTCCCGACCATGGCCATCCAGATGACCGCCATCGGCGAGGAGTCCGG
TTCGCTGGATGAGATGCTGGAAAAGGTCGCCTCATTCTACGAAGCAGAAGTCGACAATGCGGTGGACAACCTGACCACGC
TGATGGAGCCATTGATTATGTCGGTCATCGGCGTGCTGGTCGGCGGCCTGATTGTGGCCATGTACCTGCCAATCTTCCAG
CTGGGCGCAGTGGTCGGATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6M8FBD8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.012

99.754

0.788
  pilC Acinetobacter baylyi ADP1

61.823

100

0.618
  pilC Acinetobacter baumannii D1279779

61.965

97.783

0.606
  pilC Legionella pneumophila strain ERS1305867

54.293

97.537

0.53
  pilG Neisseria gonorrhoeae MS11

46

98.522

0.453
  pilG Neisseria meningitidis 44/76-A

46

98.522

0.453
  pilC Vibrio cholerae strain A1552

42.611

100

0.426
  pilC Vibrio campbellii strain DS40M4

40.554

97.783

0.397