Detailed information NTG0446381

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: HNE05_RS04365
• Genome accession: NZ_CP053697
• Coordinates: 958842..960551 (-)
• Length: 569 a.a.
• NCBI ID: WP_173203711.1
• Uniprot ID: A0A6M8FNE7
• Organism: Aquipseudomonas campi strain S1-A32-2
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	953016..964141	958842..960551	within	0

Gene organization within MGE regions

Location: 953016..964141

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
HNE05_RS04325 (HNE05_01935)	-	953016..953483 (+)	468	WP_173203699.1	FAD/FMN-containing dehydrogenase	-
HNE05_RS04330 (HNE05_01940)	-	953480..954169 (+)	690	WP_173203701.1	energy-coupling factor ABC transporter permease	-
HNE05_RS04335 (HNE05_01945)	-	954273..954488 (+)	216	WP_173211596.1	hypothetical protein	-
HNE05_RS04340 (HNE05_01950)	-	954631..955771 (+)	1141	Protein_853	IS481 family transposase	-
HNE05_RS04345 (HNE05_01955)	yacG	955945..956139 (-)	195	WP_173203703.1	DNA gyrase inhibitor YacG	-
HNE05_RS04350 (HNE05_01960)	coaE	956136..956747 (-)	612	WP_173203705.1	dephospho-CoA kinase	-
HNE05_RS04355 (HNE05_01965)	pilD	956748..957614 (-)	867	WP_173203707.1	prepilin peptidase	Machinery gene
HNE05_RS04360 (HNE05_01970)	pilC	957618..958838 (-)	1221	WP_173203709.1	type II secretion system F family protein	Machinery gene
HNE05_RS04365 (HNE05_01975)	pilB	958842..960551 (-)	1710	WP_173203711.1	type IV-A pilus assembly ATPase PilB	Machinery gene
HNE05_RS20545 (HNE05_01980)	pilV	960788..960973 (+)	186	WP_173203713.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
HNE05_RS04375 (HNE05_01985)	-	960990..961445 (+)	456	WP_173203716.1	hypothetical protein	-
HNE05_RS04380 (HNE05_01990)	-	961591..962379 (+)	789	WP_240008815.1	hypothetical protein	-
HNE05_RS04385 (HNE05_01995)	-	962382..963221 (+)	840	WP_173203718.1	hypothetical protein	-
HNE05_RS04390 (HNE05_02000)	-	963221..964141 (+)	921	WP_173203720.1	ABC transporter ATP-binding protein	-

Sequence

Protein

• Length: 569 a.a.
• Molecular weight: 62476.46 Da
• Isoelectric Point: 5.5826

>NTDB_id=446381 HNE05_RS04365 WP_173203711.1 958842..960551(-) (pilB) [Aquipseudomonas campi strain S1-A32-2]
MNDIPALTGLARQLVTAELLDERTAAQAHAQALRNKIPLVSYLVQNKLATSRAVAEIAAEQFGVPFCDLSAIDKESQPRD
LVSEKLCRQHRVLPLYRRGNKLFVALSDPTNHQAVTDIQFSTGISTEALLVEDDKLGDALEKFFDAGNSGMEDLADVDLD
GLDVEAVNDDKDDAGGGGGEAADDAPVVRFINKMLLDAIKGGSSDLHFEPYEKAYRVRFRTDGILHEVARPPIQLASRIS
ARLKVMASLDISERRKPQDGRIKMKISKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEEQKELYLNALKQ
PQGMILVTGPTGSGKTVSLYTGINILNTVDINISTAEDPVEINLEGINQVNVNPKHGMDFSQALRAFLRQDPDVIMVGEV
RDLETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSSCKKEVEIPRETLVKEG
FPEDKIGSFKIYAPVGCEHCKHGYKGRVGIYEVVKITPALQRLIMEEGNSIEIAKQAKNDGFNDLRTSGLFKAMQGITSL
EEVNRVTKD

Nucleotide

• Length: 1710 bp

>NTDB_id=446381 HNE05_RS04365 WP_173203711.1 958842..960551(-) (pilB) [Aquipseudomonas campi strain S1-A32-2]
ATGAACGATATCCCCGCCCTGACGGGCCTGGCTCGCCAACTGGTAACCGCCGAACTGCTGGACGAGCGCACAGCCGCCCA
GGCCCACGCCCAGGCCTTGCGCAATAAGATCCCCCTAGTTTCTTACCTGGTGCAGAACAAACTCGCTACCAGCAGGGCCG
TGGCAGAGATAGCAGCAGAACAATTCGGCGTTCCTTTCTGCGACCTCAGCGCCATCGATAAGGAAAGCCAGCCACGCGAC
CTGGTCAGCGAAAAACTGTGCCGCCAACACCGCGTGCTGCCGCTGTACCGTCGCGGCAACAAATTATTCGTTGCGCTATC
CGATCCAACCAACCACCAGGCTGTCACCGACATCCAGTTCAGCACCGGCATCAGCACCGAGGCCCTGCTGGTTGAAGACG
ATAAGCTGGGCGATGCGCTGGAAAAATTCTTCGATGCCGGCAATAGCGGCATGGAAGACCTGGCCGATGTCGATCTCGAC
GGTCTGGATGTTGAAGCCGTCAATGACGACAAGGACGACGCTGGCGGTGGCGGTGGCGAGGCCGCAGACGATGCGCCAGT
GGTGCGATTCATCAACAAAATGCTGCTGGATGCGATCAAAGGCGGCTCATCGGACTTGCACTTCGAGCCCTATGAAAAAG
CCTATCGGGTGCGCTTTCGCACGGACGGTATCCTTCATGAAGTAGCCAGACCGCCGATTCAACTGGCTAGCCGCATCTCC
GCCCGTCTCAAGGTAATGGCGTCTCTGGACATCTCCGAGCGACGCAAGCCGCAGGATGGCCGGATCAAGATGAAAATATC
CAAGACCAAGGCCATCGACTTTCGCGTCAATACACTCCCCACGCTATGGGGAGAGAAAATCGTCATGCGGATTCTCGACC
CCTCCAGCGCGCAGATGGGCATCGATGCCCTGGGTTATGAGGAGGAACAGAAGGAGCTGTATCTCAACGCACTGAAGCAA
CCACAAGGCATGATCCTGGTGACCGGCCCGACCGGTTCTGGCAAAACGGTGTCCCTGTATACCGGGATCAACATCCTCAA
CACCGTCGACATCAATATCTCCACCGCCGAAGACCCGGTGGAAATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACC
CCAAGCATGGCATGGACTTCTCCCAAGCCCTGCGCGCCTTCCTACGCCAGGACCCCGACGTAATCATGGTCGGCGAGGTG
CGCGACCTGGAAACCGCCGAGATTGCCATCAAGGCCGCGCAAACTGGGCACATGGTGATGTCCACCCTGCACACCAACAG
CGCCGCAGAAACCCTGACCCGCCTACGCAATATGGGCGTACCGGCCTTCAACATCGCCACCTCGGTCAACCTGATCATCG
CCCAGCGCCTGGCGCGCAAGCTGTGCAGCAGCTGCAAGAAGGAAGTCGAGATTCCAAGGGAGACCCTAGTCAAGGAAGGC
TTCCCCGAAGACAAGATCGGCAGCTTCAAGATTTATGCACCCGTCGGTTGCGAGCACTGCAAGCATGGCTACAAAGGCCG
TGTGGGTATTTATGAAGTAGTTAAAATCACACCGGCCTTGCAACGGCTTATCATGGAGGAAGGCAACTCCATTGAAATCG
CCAAGCAAGCGAAAAATGACGGTTTCAATGACCTGCGCACATCCGGGCTGTTCAAGGCGATGCAAGGCATCACCAGCCTG
GAAGAAGTCAACCGCGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (43-146)

• Type II/IV secretion system protein (T2SSE) (193-460)

3D structure

• AlphaFold DB: A0A6M8FNE7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	58.979	99.824	0.589
pilB	Acinetobacter baylyi ADP1	58.554	99.649	0.583
pilB	Legionella pneumophila strain ERS1305867	55.908	99.649	0.557
pilB	Vibrio cholerae strain A1552	50.176	99.824	0.501
pilF	Neisseria gonorrhoeae MS11	48.944	99.824	0.489
pilB	Vibrio campbellii strain DS40M4	48.94	99.473	0.487
pilB	Vibrio parahaemolyticus RIMD 2210633	51.402	94.025	0.483
pilB	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.941	92.97	0.362