Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   GYM54_RS21890 Genome accession   NZ_CP048411
Coordinates   3937253..3937429 (-) Length   58 a.a.
NCBI ID   WP_181100122.1    Uniprot ID   -
Organism   Pseudomonas sp. MTM4     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 3932253..3942429
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYM54_RS18085 (GYM54_18080) - 3932584..3932978 (-) 395 Protein_3589 nucleotidyltransferase domain-containing protein -
  GYM54_RS18090 (GYM54_18085) - 3933150..3934187 (-) 1038 WP_181100112.1 acyltransferase -
  GYM54_RS18095 (GYM54_18090) - 3934208..3935095 (-) 888 WP_181100114.1 ABC transporter ATP-binding protein -
  GYM54_RS18100 (GYM54_18095) - 3935092..3935925 (-) 834 WP_181100116.1 hypothetical protein -
  GYM54_RS18105 (GYM54_18100) - 3935922..3936722 (-) 801 WP_181100118.1 hypothetical protein -
  GYM54_RS18110 (GYM54_18105) - 3936781..3937233 (-) 453 WP_181100120.1 hypothetical protein -
  GYM54_RS21890 (GYM54_18110) pilA 3937253..3937429 (-) 177 WP_181100122.1 type IV pilin protein Machinery gene
  GYM54_RS18120 (GYM54_18115) pilB 3937941..3939644 (+) 1704 WP_181100124.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GYM54_RS18125 (GYM54_18120) pilC 3939647..3940870 (+) 1224 WP_181100126.1 type II secretion system F family protein Machinery gene
  GYM54_RS18130 (GYM54_18125) pilD 3940875..3941738 (+) 864 WP_181100129.1 A24 family peptidase Machinery gene

Sequence


Protein


Download         Length: 58 a.a.        Molecular weight: 6225.27 Da        Isoelectric Point: 9.7375

>NTDB_id=421179 GYM54_RS21890 WP_181100122.1 3937253..3937429(-) (pilA) [Pseudomonas sp. MTM4]
MKKQKGFTLIELMIVVAIIGILAAVAIPQFQDYQAKGYDKSAQSDARNILTGGIANSN

Nucleotide


Download         Length: 177 bp        

>NTDB_id=421179 GYM54_RS21890 WP_181100122.1 3937253..3937429(-) (pilA) [Pseudomonas sp. MTM4]
ATGAAGAAGCAAAAAGGCTTTACCCTGATTGAGCTGATGATCGTGGTCGCGATCATCGGAATCCTGGCTGCAGTCGCCAT
TCCGCAATTCCAGGATTACCAAGCCAAGGGCTACGACAAGTCGGCGCAATCCGACGCTCGTAATATCCTGACCGGCGGCA
TCGCCAACTCCAACTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

67.308

89.655

0.603

  pilA Pseudomonas aeruginosa PAK

76.19

72.414

0.552

  pilA Ralstonia pseudosolanacearum GMI1000

60

86.207

0.517

  pilA Vibrio campbellii strain DS40M4

80.556

62.069

0.5

  pilA Acinetobacter nosocomialis M2

80.556

62.069

0.5

  pilA Vibrio parahaemolyticus RIMD 2210633

56

86.207

0.483

  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56

86.207

0.483

  pilA2 Legionella pneumophila str. Paris

79.412

58.621

0.466

  pilA2 Legionella pneumophila strain ERS1305867

79.412

58.621

0.466

  pilA/pilA1 Eikenella corrodens VA1

77.143

60.345

0.466

  pilA Vibrio cholerae C6706

77.143

60.345

0.466

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

77.143

60.345

0.466

  pilA Vibrio cholerae strain A1552

77.143

60.345

0.466

  pilE Acinetobacter baumannii D1279779

56.522

79.31

0.448

  comF Acinetobacter baylyi ADP1

57.778

77.586

0.448

  pilA/pilAI Pseudomonas stutzeri DSM 10701

78.788

56.897

0.448

  pilE Neisseria gonorrhoeae MS11

78.788

56.897

0.448

  pilE Neisseria gonorrhoeae strain FA1090

78.788

56.897

0.448

  comP Acinetobacter baylyi ADP1

72.222

62.069

0.448

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

75.758

56.897

0.431

  pilA/pilAII Pseudomonas stutzeri DSM 10701

58.537

70.69

0.414

  pilX Neisseria meningitidis 8013

66.667

62.069

0.414

  pilL Neisseria gonorrhoeae MS11

66.667

62.069

0.414

  pilA Haemophilus influenzae Rd KW20

69.697

56.897

0.397

  pilA Haemophilus influenzae 86-028NP

69.697

56.897

0.397

  pilA Glaesserella parasuis strain SC1401

65.625

55.172

0.362


Multiple sequence alignment