Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   GYM54_RS18125 Genome accession   NZ_CP048411
Coordinates   3939647..3940870 (+) Length   407 a.a.
NCBI ID   WP_181100126.1    Uniprot ID   -
Organism   Pseudomonas sp. MTM4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3934647..3945870
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYM54_RS18100 (GYM54_18095) - 3935092..3935925 (-) 834 WP_181100116.1 hypothetical protein -
  GYM54_RS18105 (GYM54_18100) - 3935922..3936722 (-) 801 WP_181100118.1 hypothetical protein -
  GYM54_RS18110 (GYM54_18105) - 3936781..3937233 (-) 453 WP_181100120.1 hypothetical protein -
  GYM54_RS21890 (GYM54_18110) pilA 3937253..3937429 (-) 177 WP_181100122.1 type IV pilin protein Machinery gene
  GYM54_RS18120 (GYM54_18115) pilB 3937941..3939644 (+) 1704 WP_181100124.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GYM54_RS18125 (GYM54_18120) pilC 3939647..3940870 (+) 1224 WP_181100126.1 type II secretion system F family protein Machinery gene
  GYM54_RS18130 (GYM54_18125) pilD 3940875..3941738 (+) 864 WP_181100129.1 A24 family peptidase Machinery gene
  GYM54_RS18135 (GYM54_18130) coaE 3942053..3942661 (+) 609 WP_181100131.1 dephospho-CoA kinase -
  GYM54_RS18140 (GYM54_18135) yacG 3942658..3942852 (+) 195 WP_181100133.1 DNA gyrase inhibitor YacG -
  GYM54_RS18145 (GYM54_18140) - 3942862..3943554 (-) 693 WP_131649934.1 energy-coupling factor ABC transporter permease -
  GYM54_RS18150 (GYM54_18145) - 3943633..3944670 (-) 1038 WP_131649935.1 NADP(H)-dependent aldo-keto reductase -
  GYM54_RS18155 (GYM54_18150) rplM 3945065..3945493 (+) 429 WP_019342644.1 50S ribosomal protein L13 -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 44690.48 Da        Isoelectric Point: 9.9689

>NTDB_id=421169 GYM54_RS18125 WP_181100126.1 3939647..3940870(+) (pilC) [Pseudomonas sp. MTM4]
MAQQKALKTSVFTWEGTDRKGSRIKGELSGQSPALIKAQLRKQGINPLKVRKKAVSLFSAGKKIKPMDIALFTRQMATMM
KAGVPLLQSFDIIGEGFDNSNMRKLVDDVKQEVAAGNSFAGSLRKKPQYFDDLYCNLVESGEQSGALETLLDRVATYKEK
TEQLKAKIRKAMTYPIAVIVVAVIVSAILLIKVVPQFENVFHGFGAELPAFTMMVIGLSRWLQDWWYLVIVGMFAAAFLF
KHSYKRSEKFRDVLDRGLLRLPIVGNILYKAVVARYARTLSTTFAAGVPLVDALDSVGGATGNVVFRNAVAKIRNDVSSG
VQLNFSMRTTAVFPSMAVQMTAIGEESGSLDEMLDKVAGFYEDEVDNMVDNLTTLMEPMIMAVLGVLVGGLIIAMYLPIF
QLGSVIG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=421169 GYM54_RS18125 WP_181100126.1 3939647..3940870(+) (pilC) [Pseudomonas sp. MTM4]
ATGGCGCAGCAGAAAGCGTTGAAAACCAGTGTGTTCACCTGGGAAGGCACTGACCGCAAGGGCAGCAGGATCAAGGGAGA
GCTGTCTGGCCAGAGCCCGGCGCTGATCAAGGCGCAACTGCGCAAGCAGGGCATTAATCCGCTTAAGGTGCGCAAGAAAG
CGGTCTCGCTGTTCAGCGCGGGCAAGAAGATCAAGCCGATGGACATCGCGCTGTTCACCCGGCAGATGGCGACCATGATG
AAGGCCGGTGTGCCGTTGTTGCAGTCGTTCGACATCATCGGTGAGGGCTTCGACAATTCGAACATGCGCAAGCTGGTGGA
TGACGTGAAGCAAGAGGTGGCCGCTGGTAACAGCTTCGCCGGTTCGTTGCGCAAGAAGCCGCAATATTTCGATGACCTTT
ATTGCAACCTGGTGGAATCCGGTGAGCAGTCCGGCGCGCTGGAAACCTTGCTGGATAGGGTCGCAACCTACAAGGAAAAG
ACCGAGCAGCTCAAGGCTAAGATTAGAAAGGCAATGACTTATCCGATAGCAGTTATTGTGGTGGCGGTCATAGTTTCCGC
GATCCTGCTGATCAAGGTGGTGCCACAGTTTGAGAATGTATTTCACGGTTTCGGTGCGGAACTGCCTGCTTTCACCATGA
TGGTGATTGGGCTCTCGCGCTGGCTGCAGGACTGGTGGTATCTGGTTATCGTTGGGATGTTTGCGGCGGCATTTCTATTC
AAGCATAGCTATAAGCGCTCGGAAAAATTTCGCGATGTGCTGGATAGAGGGCTCCTCAGGTTGCCGATTGTAGGCAACAT
TCTTTACAAGGCCGTTGTGGCGCGTTACGCGCGAACTCTTTCGACTACCTTCGCTGCCGGCGTACCACTTGTCGATGCGC
TGGATTCGGTAGGGGGTGCCACTGGTAACGTAGTGTTCCGCAACGCGGTCGCTAAAATTCGTAACGATGTGTCCTCCGGT
GTGCAGCTTAATTTCTCGATGCGTACTACCGCGGTGTTCCCCTCAATGGCGGTTCAGATGACGGCCATTGGTGAGGAGTC
AGGCTCGCTGGACGAAATGCTGGATAAAGTTGCGGGTTTCTACGAGGACGAAGTGGACAATATGGTCGATAACCTGACGA
CTCTGATGGAACCCATGATCATGGCGGTGCTGGGGGTGCTGGTGGGGGGGCTGATCATCGCGATGTACCTGCCTATCTTC
CAGCTTGGCAGCGTGATCGGTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(277-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

82.63

99.017

0.818
  pilC Acinetobacter baylyi ADP1

61.275

100

0.614
  pilC Acinetobacter baumannii D1279779

59.559

100

0.597
  pilC Legionella pneumophila strain ERS1305867

51.768

97.297

0.504
  pilG Neisseria gonorrhoeae MS11

43.035

98.771

0.425
  pilG Neisseria meningitidis 44/76-A

42.928

99.017

0.425
  pilC Vibrio cholerae strain A1552

41.912

100

0.42
  pilC Vibrio campbellii strain DS40M4

41.31

97.543

0.403
  pilC Thermus thermophilus HB27

37.157

98.526

0.366

Multiple sequence alignment