Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   GYM54_RS18120 Genome accession   NZ_CP048411
Coordinates   3937941..3939644 (+) Length   567 a.a.
NCBI ID   WP_181100124.1    Uniprot ID   -
Organism   Pseudomonas sp. MTM4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3932941..3944644
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  GYM54_RS18090 (GYM54_18085) - 3933150..3934187 (-) 1038 WP_181100112.1 acyltransferase -
  GYM54_RS18095 (GYM54_18090) - 3934208..3935095 (-) 888 WP_181100114.1 ABC transporter ATP-binding protein -
  GYM54_RS18100 (GYM54_18095) - 3935092..3935925 (-) 834 WP_181100116.1 hypothetical protein -
  GYM54_RS18105 (GYM54_18100) - 3935922..3936722 (-) 801 WP_181100118.1 hypothetical protein -
  GYM54_RS18110 (GYM54_18105) - 3936781..3937233 (-) 453 WP_181100120.1 hypothetical protein -
  GYM54_RS21890 (GYM54_18110) pilA 3937253..3937429 (-) 177 WP_181100122.1 type IV pilin protein Machinery gene
  GYM54_RS18120 (GYM54_18115) pilB 3937941..3939644 (+) 1704 WP_181100124.1 type IV-A pilus assembly ATPase PilB Machinery gene
  GYM54_RS18125 (GYM54_18120) pilC 3939647..3940870 (+) 1224 WP_181100126.1 type II secretion system F family protein Machinery gene
  GYM54_RS18130 (GYM54_18125) pilD 3940875..3941738 (+) 864 WP_181100129.1 A24 family peptidase Machinery gene
  GYM54_RS18135 (GYM54_18130) coaE 3942053..3942661 (+) 609 WP_181100131.1 dephospho-CoA kinase -
  GYM54_RS18140 (GYM54_18135) yacG 3942658..3942852 (+) 195 WP_181100133.1 DNA gyrase inhibitor YacG -
  GYM54_RS18145 (GYM54_18140) - 3942862..3943554 (-) 693 WP_131649934.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62430.39 Da        Isoelectric Point: 5.4555

>NTDB_id=421168 GYM54_RS18120 WP_181100124.1 3937941..3939644(+) (pilB) [Pseudomonas sp. MTM4]
MNENAPLGGLARQLVLVGQLDEKVAQQAQQQAQRNQVSLVSWLVQNKLIKSRTVVDVAGEQFGVPYFDLSALDRETQPTD
LISEKLVRQHRLLPLQRRGNKLYVAISDPTNHQAISDIQFNTGLSVESVLVEDDKLGEAIEKLFESADAAMDGLTDSDLD
ELDVEISGGANEEDGGHNEADDAPVVRFVNKMLLDAIKRGSSDLHFEPYEKSYRVRFRTDGVLHEVARPPVQLVSRISAR
LKVMAALDIAERRKPQDGRIKMKVSKSKSIDFRVNSLPTLWGEKIVMRILDSSSAKMGIDALGYEEEQKALYMAALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTIDVNISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPGCKKPVEVPRDALLKEGFP
EDKIGTFQLYGPVGCDNCNGGYKGRVGIYEVVKITPALQRLIMEEGNSIDIAIQARRDGFNDLRLSGLVKAMHGITSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=421168 GYM54_RS18120 WP_181100124.1 3937941..3939644(+) (pilB) [Pseudomonas sp. MTM4]
ATGAACGAAAACGCTCCTCTCGGTGGTTTAGCTCGCCAGTTGGTATTGGTTGGTCAGCTTGATGAAAAGGTCGCGCAGCA
GGCCCAGCAACAGGCCCAGCGTAATCAGGTGTCGCTGGTTTCCTGGTTGGTGCAGAACAAACTGATAAAAAGTCGGACGG
TGGTGGATGTCGCTGGTGAGCAGTTTGGCGTTCCGTATTTCGATCTGAGTGCTCTGGACAGGGAAACCCAGCCTACGGAC
CTGATTAGCGAAAAGCTGGTACGCCAGCACCGTCTGCTTCCACTGCAGCGTCGCGGTAACAAATTGTATGTCGCGATTTC
CGATCCAACTAATCATCAGGCCATCAGCGATATCCAGTTCAACACCGGGCTTTCTGTCGAATCGGTGCTGGTTGAAGATG
ACAAGCTTGGCGAAGCGATTGAAAAACTGTTCGAGTCTGCCGACGCTGCGATGGATGGGCTTACTGATTCTGATCTCGAC
GAGTTGGATGTCGAGATTAGTGGTGGCGCCAATGAAGAGGATGGAGGGCACAACGAGGCCGATGATGCGCCGGTTGTGCG
CTTCGTCAACAAGATGCTGCTAGACGCAATCAAGCGCGGCTCGTCGGACCTGCACTTCGAACCCTACGAGAAATCCTATC
GGGTGCGCTTCCGTACCGACGGTGTGCTGCATGAGGTAGCGAGGCCGCCAGTGCAGTTGGTCAGTCGAATTTCCGCGCGC
CTCAAGGTCATGGCGGCGCTGGATATTGCCGAGCGGCGCAAGCCGCAGGACGGCCGGATCAAGATGAAGGTCTCTAAGAG
CAAGTCCATCGACTTCCGTGTCAACAGCCTGCCGACGCTATGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCATCCA
GCGCCAAGATGGGCATCGATGCGCTGGGCTATGAGGAAGAGCAAAAAGCGCTGTACATGGCTGCGCTGAAGCAGCCGCAG
GGGATGATTTTGGTCACCGGCCCAACGGGATCGGGCAAGACGGTTTCGCTGTACACCGGCCTGAATATTCTCAATACCAT
CGATGTGAATATTTCTACTGCTGAAGACCCGGTGGAGATCAACCTCGAAGGCATCAATCAGGTCAACGTCAATCCGCGTC
AGGGCATGGACTTTGCCCAGGCGTTACGCGCCTTTTTGCGTCAGGACCCGGACATCATCATGGTCGGCGAAATCCGCGAT
CTGGAAACCGCCGAGATCGCCATCAAGGCGGCGCAGACAGGCCATATGGTGATGTCAACGCTGCATACCAACAGTGCCGC
TGAAACCTTGACCCGCCTGCGCAATATGGGGGTGCCGGCATTCAATATCGCGACTTCGGTCAACTTGATCATCGCCCAGC
GTTTGGCGCGCAAGTTGTGTCCCGGTTGCAAGAAGCCGGTCGAGGTGCCGCGCGACGCCTTGCTGAAGGAAGGCTTTCCA
GAAGACAAGATCGGCACGTTCCAGCTTTATGGGCCGGTGGGTTGTGACAATTGCAATGGCGGTTACAAGGGCCGTGTCGG
TATTTATGAAGTGGTTAAAATCACGCCTGCTCTACAGCGGCTTATCATGGAGGAAGGAAACTCCATCGATATCGCTATTC
AAGCGCGCAGAGATGGCTTCAATGATTTGCGTCTTTCGGGTCTGGTCAAAGCCATGCACGGCATCACCAGCCTGGAGGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

59.929

99.471

0.596
  pilB Acinetobacter baumannii D1279779

59.123

100

0.594
  pilB Legionella pneumophila strain ERS1305867

54.021

100

0.545
  pilB Vibrio cholerae strain A1552

51.197

95.767

0.49
  pilF Neisseria gonorrhoeae MS11

48.85

99.647

0.487
  pilB Vibrio parahaemolyticus RIMD 2210633

51.21

94.709

0.485
  pilB Vibrio campbellii strain DS40M4

50.464

95.062

0.48
  pilF Thermus thermophilus HB27

37.5

100

0.376
  pilB/pilB1 Synechocystis sp. PCC 6803

39.575

91.358

0.362

Multiple sequence alignment