Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   CAP2UW1_RS06635 Genome accession   NC_013194
Coordinates   1507720..1507905 (+) Length   61 a.a.
NCBI ID   WP_015765886.1    Uniprot ID   A0A9D8KUC6
Organism   Candidatus Accumulibacter phosphatis     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 1502720..1512905
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAP2UW1_RS06615 (CAP2UW1_1325) - 1503994..1504491 (-) 498 WP_015765882.1 hypothetical protein -
  CAP2UW1_RS06620 (CAP2UW1_1326) - 1504722..1505171 (-) 450 WP_015765883.1 DUF29 domain-containing protein -
  CAP2UW1_RS23590 (CAP2UW1_1327) - 1505276..1506847 (-) 1572 WP_015765884.1 hydrogenase iron-sulfur subunit -
  CAP2UW1_RS06630 (CAP2UW1_1328) - 1507114..1507326 (+) 213 WP_291933855.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  CAP2UW1_RS06635 (CAP2UW1_1329) pilA 1507720..1507905 (+) 186 WP_015765886.1 type IV pilin protein Machinery gene
  CAP2UW1_RS25355 (CAP2UW1_1330) - 1507933..1508322 (+) 390 WP_015765887.1 hypothetical protein -
  CAP2UW1_RS06645 (CAP2UW1_1331) - 1508388..1509191 (+) 804 WP_015765888.1 ABC transporter permease -
  CAP2UW1_RS06650 (CAP2UW1_1332) - 1509193..1510044 (+) 852 WP_015765889.1 hypothetical protein -
  CAP2UW1_RS06655 (CAP2UW1_1333) - 1510041..1510952 (+) 912 WP_015765890.1 ABC transporter ATP-binding protein -
  CAP2UW1_RS06660 (CAP2UW1_1334) - 1511073..1512203 (-) 1131 WP_015765891.1 phosphatase PAP2 family protein -

Sequence


Protein


Download         Length: 61 a.a.        Molecular weight: 6576.72 Da        Isoelectric Point: 8.6385

>NTDB_id=35147 CAP2UW1_RS06635 WP_015765886.1 1507720..1507905(+) (pilA) [Candidatus Accumulibacter phosphatis]
MKKLQKGFTLIELMIVVAIIGILAAIAIPQFSDYTRKGEDKAAQSDARNILTSAVVNSSPE

Nucleotide


Download         Length: 186 bp        

>NTDB_id=35147 CAP2UW1_RS06635 WP_015765886.1 1507720..1507905(+) (pilA) [Candidatus Accumulibacter phosphatis]
ATGAAGAAGCTGCAGAAAGGCTTTACCCTGATCGAACTGATGATCGTTGTGGCCATCATCGGTATTCTGGCCGCGATCGC
CATTCCCCAATTCTCTGACTACACGCGCAAAGGCGAGGATAAAGCGGCCCAGTCGGACGCGAGAAATATCCTGACCTCGG
CCGTCGTCAACTCGTCCCCAGAGTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

64.706

83.607

0.541

  comP Acinetobacter baylyi ADP1

56.14

93.443

0.525

  pilA Acinetobacter nosocomialis M2

58.491

86.885

0.508

  pilA Acinetobacter baumannii strain A118

64.583

78.689

0.508

  pilA Pseudomonas aeruginosa PAK

73.171

67.213

0.492

  pilA/pilAI Pseudomonas stutzeri DSM 10701

70.732

67.213

0.475

  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

54.717

86.885

0.475

  pilE Acinetobacter baumannii D1279779

61.702

77.049

0.475

  pilA2 Legionella pneumophila strain ERS1305867

90.323

50.82

0.459

  pilA2 Legionella pneumophila str. Paris

90.323

50.82

0.459

  pilA Vibrio campbellii strain DS40M4

75.676

60.656

0.459

  pilA/pilA1 Eikenella corrodens VA1

71.795

63.934

0.459

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

80

57.377

0.459

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

77.143

57.377

0.443

  pilA Vibrio cholerae strain A1552

77.143

57.377

0.443

  pilA Vibrio cholerae C6706

77.143

57.377

0.443

  pilA Vibrio parahaemolyticus RIMD 2210633

50.943

86.885

0.443

  pilE Neisseria gonorrhoeae strain FA1090

66.667

63.934

0.426

  pilE Neisseria gonorrhoeae MS11

66.667

63.934

0.426

  pilA Haemophilus influenzae Rd KW20

67.568

60.656

0.41

  pilA Haemophilus influenzae 86-028NP

69.444

59.016

0.41

  pilA Glaesserella parasuis strain SC1401

64.865

60.656

0.393

  comE Acinetobacter baylyi ADP1

48

81.967

0.393

  pilA/pilAII Pseudomonas stutzeri DSM 10701

68.571

57.377

0.393

  comF Acinetobacter baylyi ADP1

71.875

52.459

0.377

  pilL Neisseria gonorrhoeae MS11

67.647

55.738

0.377

  pilX Neisseria meningitidis 8013

67.647

55.738

0.377

  comYC Streptococcus gordonii str. Challis substr. CH1

35.484

100

0.361

  pilA/pilA1 Synechocystis sp. PCC 6803

50

72.131

0.361


Multiple sequence alignment