Detailed information of T6SS

Note: only experimentally validated proteins are displayed in the graph.


Summary

T6SS ID T6SS00258 experimental
T6SS type Type i3
Strain Yersinia pseudotuberculosis YPIII
Replicon (RefSeq) chromosome (NC_010465)
Location 1619849..1653371
Function anti-bacterial
Comment The secretion of YPK_0952 was dramatically diminished with deletion of clpV3, but not with deletion of clpV1, clpV2,or clpV4, further indicating that YPK_0952 is a T6SS effector mainly associated with T6SS-3.
References
[1] Zhang W et al (2011) Modulation of a thermoregulated type VI secretion system by AHL-dependent quorum sensing in Yersinia pseudotuberculosis. Arch Microbiol. 193(5):351-63. [PudMed:21298257]
[2] Zhang W et al (2012) A type VI secretion system regulated by OmpR in Yersinia pseudotuberculosis functions to maintain intracellular pH homeostasis. Environ Microbiol. 15(2):557-69. [PudMed:23094603]
[3] Song L, Pan J, Yang Y, Zhang Z, Cui R, Jia S, Wang Z, Yang C, Xu L, Dong TG, Wang Y, Shen X. et al (2021) Contact-independent killing mediated by a T6SS effector with intrinsic cell-entry properties.. Nat Commun. 12(1):423. [PudMed:33462232]
experimental Experimental investigation has been performed.

T6SS components and genome coordinates

ComponentPAARTagJTagLTssATssBTssCTssDTssETssFTssGTssHTssITssJTssKTssLTssM
Number1111112111111111


Component ID Component Locus tag (Gene) Coordinate Strand Size (bp) NCBI ID Product
YPK_RS07385 (mltB) 1616059..1617153 + 1095 WP_012303905.1 lytic murein transglycosylase B
YPK_RS07390 1618395..1618997 + 603 WP_011192718.1 LuxR family transcriptional regulator
YPK_RS07395 1619370..1619852 - 483 WP_002215321.1 DcrB-related protein
T6CP011650 TagL YPK_RS07400 1619849..1621531 - 1683 WP_012104863.1 OmpA family protein
YPK_RS07405 1621532..1622233 - 702 WP_012303906.1 hypothetical protein
YPK_RS07410 1622230..1623567 - 1338 WP_012104865.1 glycosyl transferase
YPK_RS07415 1623607..1624638 - 1032 WP_011192714.1 fimbrial protein
T6CP002544 TssA YPK_RS07420 1624645..1625838 - 1194 WP_011192713.1 ImpA family type VI secretion system protein
T6CP002545 TssG YPK_RS07425 (tssG) 1625937..1626962 - 1026 WP_012303907.1 type VI secretion system baseplate subunit TssG
T6CP002546 TssF YPK_RS07430 (tssF) 1626962..1628842 - 1881 WP_002211582.1 type VI secretion system baseplate subunit TssF
T6CP002547 TssH YPK_RS07435 (tssH) 1629626..1632283 + 2658 WP_012104866.1 type VI secretion system ATPase TssH
YPK_RS07440 1632494..1633039 + 546 WP_002211580.1 type 1 fimbrial protein
YPK_RS07445 1633196..1633957 + 762 WP_012303908.1 fimbria/pilus periplasmic chaperone
YPK_RS21995 1634085..1636757 + 2673 WP_012303909.1 fimbrial biogenesis outer membrane usher protein
YPK_RS07460 1636742..1637350 + 609 WP_011192708.1 type 1 fimbrial protein
T6CP002548 TssB YPK_RS07465 (tssB) 1637456..1637980 + 525 WP_002211575.1 type VI secretion system contractile sheath small subunit
T6CP002549 TssC YPK_RS07470 (tssC) 1638004..1639506 + 1503 WP_002211574.1 type VI secretion system contractile sheath large subunit
T6CP002550 TssD YPK_RS07475 1639839..1640324 + 486 WP_002211573.1 type VI secretion system tube protein Hcp
T6CP002551 TssJ YPK_RS07480 (tssJ) 1640598..1641137 + 540 WP_002211572.1 type VI secretion system lipoprotein TssJ
T6CP002552 TssK YPK_RS07485 (tssK) 1641141..1642490 + 1350 WP_002211571.1 type VI secretion system baseplate subunit TssK
T6CP002553 TssL YPK_RS07490 (tssL) 1642487..1643863 + 1377 WP_011192706.1 type VI secretion system protein TssL, long form
T6CP002554 TssM YPK_RS07495 (tssM) 1643872..1647699 + 3828 WP_011192705.1 type VI secretion system membrane subunit TssM
T6CP002555 TssI YPK_RS07500 1648026..1650794 + 2769 WP_012303910.1 type VI secretion system Vgr family protein
T6CP011649 PAAR YPK_RS07505 1650893..1651153 + 261 WP_002209684.1 PAAR domain-containing protein
YPK_RS07510 1651159..1652001 + 843 WP_002354537.1 TagK domain-containing protein
T6CP012755 TagJ YPK_RS07515 1652021..1652827 + 807 WP_012303911.1 virulence protein SciE type
T6CP002556 TssE YPK_RS07520 1652892..1653371 + 480 WP_002209686.1 type VI secretion system baseplate subunit TssE
YPK_RS07525 1653396..1653743 + 348 WP_002209687.1 hypothetical protein
YPK_RS07530 1653818..1654159 - 342 WP_002209688.1 alkylphosphonate utilization protein
YPK_RS07535 1654535..1656574 - 2040 WP_012303912.1 DUF2207 domain-containing protein
YPK_RS07540 1656579..1657121 - 543 WP_002209691.1 LemA family protein
YPK_RS07550 1657738..1658205 + 468 WP_012303913.1 hypothetical protein
flank Genes in the 5-Kb flanking regions if available, or non-core components encoded by the T6SS gene cluster if any.
Download FASTA format files

    

Note: in the 'Target' column, ↓ means the target is positively regulated by the regulator, while ↑ indicates negatively regulation.
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Regulator ID Locus tag (Gene) Regulator type Regulatory pathway Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Target (Regulation)

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Effector ID Locus tag (Gene) Classification Cognate immunity protein Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID
EFF01873 experimental YPK_RS04780 Tde - Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 1067938..1069470 (+) 499506274
EFF01874 experimental YPK_0954 (Tce1) Tde IMU00376 experimental Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 1069948..1070205 (+) 488156855

experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed.
Immunity protein ID Locus tag (Gene) Cognate effector Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID

experimental Experimental investigation has been performed.
Accessory protein ID Locus tag (Gene) Type             Organism                 Replicon (Accession) Coordinates (Strand) NCBI ID Related structure protein Related effector
ACP00014 experimental YPK_0782 (btuB) chaperone Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 1069948..1070205 (+) 488156855 - YPK_0954 (Tce1)
ACP00015 experimental YPK_2649 (ompF) chaperone Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 2911078..2912160 (+) 501261276 - YPK_0954 (Tce1)
ACP00016 experimental YPK_2956 (tonB) chaperone Yersinia pseudotuberculosis YPIII chromosome (NC_010465) 3254339..3255631 (-) 488139530 - YPK_0954 (Tce1)