Detailed information of accessory protein


Summary

Accessory protein ID ACP00015 experimental
Type chaperone
Related T6SS (Type) T6SS00258 (Type i3)
Strain Yersinia pseudotuberculosis YPIII
Replicon chromosome
Related structure protein -
Related effector YPK_0954 (Tce1)
Description GST pull-down assays showed proteins specifically retained by GST-Tce1 were detected and mass spectrometric analysis identified Tce1 partners: BtuB, , OmpF , TolB.The specific interactions of Tce1 with BtuB, OmpF, and TolB were further supported by bacterial two-hybrid and in vitro binding assays. BtuB and OmpF are important for contact-independent, but not for contact-dependent, entry of Tce1.
Reference [1] Song L, Pan J, Yang Y, et al. Contact-independent killing mediated by a T6SS effector with intrinsic cell-entry properties. Nat Commun. 2021 Jan 18;12(1):423. doi: 10.1038/s41467-020-20726-8. PMID: 33462232
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed
External database links

Locus tag (Gene) YPK_2649 (ompF)
Coordinates (Strand) 2911078..2912160 (+)
NCBI ID 501261276
RefSeq NC_010465
Uniprot ID A0A0H3B637_YERPY
KEGG ID ypy:YPK_2649
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Porin_1 PF00267.23 1.6e-141 28..360
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-6 MMKRNI
Transmembrane helix 7-26 LAVVIPALLAAGAANAAEIY
Outside 27-360 NKDGNKLDLYGKVDARHSFSDNNKQDGDKSYVRFGFKGETQITDQLTGYGQWEYNIQANNAEESGAQEGNATRLGFAGLKFAEFGSFDYGRNYGVIYDVNAWTDMLPVFGGDSISNSDNFMTGRSTGLATYRNNNFFGLVDGLNFALQYQGKNDRSEVKEANGDGFGIGSTYDIGNGINFGAGFSSSNRTLDQKYGSTAEGDKAQAWNVGAKYDANNVYLAVMYAETQNLTPYGSFSDTIANKTRDIEITAQYQFDFGLRPSLGYVQSKGKDLNDVDANHDLLKYVSVGTYYYFNKNMSTYVDYKINLLDEDEFTIANGLNTDNVVAVGLVYQF


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
SP(Sec/SPI) 0.9979 pos: 22-23. ANA-AE. MMKRNILAVVIPALLAAGAANA


  Protein sequence: 361 a.a.    

>ACP00015 NC_010465:2911078-2912160 [Yersinia pseudotuberculosis YPIII]
MMKRNILAVVIPALLAAGAANAAEIYNKDGNKLDLYGKVDARHSFSDNNKQDGDKSYVRFGFKGETQITDQLTGYGQWEY
NIQANNAEESGAQEGNATRLGFAGLKFAEFGSFDYGRNYGVIYDVNAWTDMLPVFGGDSISNSDNFMTGRSTGLATYRNN
NFFGLVDGLNFALQYQGKNDRSEVKEANGDGFGIGSTYDIGNGINFGAGFSSSNRTLDQKYGSTAEGDKAQAWNVGAKYD
ANNVYLAVMYAETQNLTPYGSFSDTIANKTRDIEITAQYQFDFGLRPSLGYVQSKGKDLNDVDANHDLLKYVSVGTYYYF
NKNMSTYVDYKINLLDEDEFTIANGLNTDNVVAVGLVYQF*

  Nucleotide sequence: 1083 bp    

>ACP00015 NC_010465:2911078-2912160 [Yersinia pseudotuberculosis YPIII]
ATGATGAAGCGCAATATTCTTGCAGTAGTAATCCCAGCTCTGTTAGCCGCTGGTGCAGCTAATGCAGCAGAAATCTATAA
CAAAGATGGCAACAAACTTGACCTGTACGGTAAAGTTGACGCACGTCACTCTTTCTCCGATAACAACAAACAAGATGGCG
ACAAGTCTTACGTTCGTTTCGGCTTCAAAGGTGAAACCCAAATTACTGACCAACTGACCGGTTACGGCCAGTGGGAATAC
AACATTCAAGCAAACAACGCTGAAGAATCAGGCGCTCAAGAAGGCAACGCAACCCGTCTGGGCTTTGCTGGTCTGAAATT
TGCTGAATTCGGTTCATTCGACTACGGCCGTAACTACGGCGTAATCTATGACGTTAACGCATGGACTGACATGCTGCCAG
TGTTCGGTGGTGATTCAATCTCCAACTCTGACAACTTCATGACTGGCCGTTCTACTGGCCTGGCAACTTATCGTAACAAC
AATTTCTTCGGTCTGGTTGACGGCCTGAACTTTGCCCTGCAATACCAAGGCAAAAATGACCGTTCTGAAGTCAAAGAAGC
TAACGGTGACGGCTTCGGTATCGGTTCTACTTATGATATCGGCAATGGCATTAACTTCGGTGCTGGTTTCTCTTCTTCAA
ACCGTACTCTTGATCAAAAATATGGTTCTACCGCTGAAGGCGACAAAGCTCAGGCATGGAACGTTGGCGCTAAATACGAC
GCAAACAACGTATACCTGGCTGTAATGTATGCTGAAACTCAGAACCTGACTCCATACGGTAGCTTCTCTGATACTATCGC
TAACAAGACTCGCGATATCGAGATCACTGCACAATATCAGTTCGACTTCGGTCTGCGCCCATCACTGGGTTATGTTCAGT
CCAAAGGTAAGGACCTGAACGATGTTGATGCCAACCATGATCTGCTGAAATATGTTTCTGTTGGTACTTACTACTACTTC
AACAAAAACATGTCTACCTATGTTGATTACAAAATCAACTTGCTGGACGAAGATGAGTTCACAATCGCAAACGGTCTGAA
CACCGACAACGTTGTTGCTGTTGGCTTGGTTTACCAGTTCTAA