Detailed information of accessory protein


Accessory protein ID ACP00014 experimental
Type chaperone
Related T6SS (Type) T6SS00258 (Type i3)
Strain Yersinia pseudotuberculosis YPIII
Replicon chromosome
Related structure protein -
Related effector YPK_0954 (Tce1)
Description GST pull-down assays showed proteins specifically retained by GST-Tce1 were detected and mass spectrometric analysis identified Tce1 partners: BtuB, , OmpF , TolB.The specific interactions of Tce1 with BtuB, OmpF, and TolB were further supported by bacterial two-hybrid and in vitro binding assays.BtuB and OmpF are important for contact-independent, but not for contact-dependent, entry of Tce1.
Reference [1] Song L, Pan J, Yang Y, et al. Contact-independent killing mediated by a T6SS effector with intrinsic cell-entry properties. Nat Commun. 2021 Jan 18;12(1):423. doi: 10.1038/s41467-020-20726-8. PMID: 33462232
experimental Experimental investigation has been performed.
insolico Bioinformatics investigation has been performed

External database links

Locus tag (Gene) YPK_0782 (btuB)
Coordinates (Strand) 1069948..1070205 (+)
NCBI ID 488156855
RefSeq NC_010465
Uniprot ID Q666X9_YERPS
KEGG ID yps:YPTB3115

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence

Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9928 - -

  Protein sequence: 86 a.a.    

>ACP00014 NC_010465:1069948-1070205 [Yersinia pseudotuberculosis YPIII]

  Nucleotide sequence: 258 bp    

>ACP00014 NC_010465:1069948-1070205 [Yersinia pseudotuberculosis YPIII]