Detailed information of accessory protein
|Accessory protein ID||ACP00014|
|Related T6SS (Type)||T6SS00258 (Type i3)|
|Strain||Yersinia pseudotuberculosis YPIII|
|Related structure protein||-|
|Related effector||YPK_0954 (Tce1)|
|Description||GST pull-down assays showed proteins specifically retained by GST-Tce1 were detected and mass spectrometric analysis identified Tce1 partners: BtuB, , OmpF , TolB.The specific interactions of Tce1 with BtuB, OmpF, and TolB were further supported by bacterial two-hybrid and in vitro binding assays.BtuB and OmpF are important for contact-independent, but not for contact-dependent, entry of Tce1.|
|Reference|| Song L, Pan J, Yang Y, et al. Contact-independent killing mediated by a T6SS effector with intrinsic cell-entry properties. Nat Commun. 2021 Jan 18;12(1):423. doi: 10.1038/s41467-020-20726-8. PMID: 33462232|
Bioinformatics investigation has been performed
External database links
|Locus tag (Gene)||YPK_0782 (btuB)|
|Coordinates (Strand)||1069948..1070205 (+)|
Pfam domain hit(s)
|Domain||Pfam ID||E-value||Aligned region|
- Transmembrane helices are predicted using TMHMM 2.0 software.
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
|Prediction||Probability||Cleavage site||Signal peptide sequence|
Protein sequence: 86 a.a.
Nucleotide sequence: 258 bp