Detailed information of accessory protein
Summary
| Accessory protein ID | ACP00014  |
| Type | chaperone |
| Related T6SS (Type) | T6SS00258 (Type i3) |
| Strain | Yersinia pseudotuberculosis YPIII |
| Replicon | chromosome |
| Related structure protein | - |
| Related effector | YPK_0954 (Tce1) |
| Description | GST pull-down assays showed proteins specifically retained by GST-Tce1 were detected and mass spectrometric analysis identified Tce1 partners: BtuB, , OmpF , TolB.The specific interactions of Tce1 with BtuB, OmpF, and TolB were further supported by bacterial two-hybrid and in vitro binding assays.BtuB and OmpF are important for contact-independent, but not for contact-dependent, entry of Tce1. |
| Reference | [1] Song L, Pan J, Yang Y, et al. Contact-independent killing mediated by a T6SS effector with intrinsic cell-entry properties. Nat Commun. 2021 Jan 18;12(1):423. doi: 10.1038/s41467-020-20726-8. PMID: 33462232 |
Experimental investigation has been performed.
Bioinformatics investigation has been performedExternal database links
| Locus tag (Gene) | YPK_0782 (btuB) |
| Coordinates (Strand) | 1069948..1070205 (+) |
| NCBI ID | 488156855 |
| RefSeq | NC_010465 |
| Uniprot ID | Q666X9_YERPS |
| KEGG ID | yps:YPTB3115 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Outside | 1-85 | MVANGNVESISLPEVRRLMSGELDRSSASEWAFAIIDDDHIRVSDQVVWKVLQCLGGADLPITDREYLYEKEDFNCWLNEIDSHE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| Other | 0.9928 | - | - |
Protein sequence: 86 a.a.
>ACP00014 NC_010465:1069948-1070205 [Yersinia pseudotuberculosis YPIII]
MVANGNVESISLPEVRRLMSGELDRSSASEWAFAIIDDDHIRVSDQVVWKVLQCLGGADLPITDREYLYEKEDFNCWLNE
IDSHE*
MVANGNVESISLPEVRRLMSGELDRSSASEWAFAIIDDDHIRVSDQVVWKVLQCLGGADLPITDREYLYEKEDFNCWLNE
IDSHE*
Nucleotide sequence: 258 bp
>ACP00014 NC_010465:1069948-1070205 [Yersinia pseudotuberculosis YPIII]
ATGGTAGCAAATGGTAACGTTGAGAGCATTTCACTGCCGGAGGTAAGGCGCTTAATGTCAGGGGAACTTGACCGCTCAAG
CGCCTCTGAATGGGCGTTTGCAATAATTGATGATGATCACATTCGAGTAAGCGATCAGGTTGTTTGGAAAGTACTTCAAT
GCTTGGGGGGAGCAGATTTACCGATAACTGACAGGGAGTATTTATATGAAAAAGAAGACTTTAATTGCTGGCTCAACGAA
ATTGACTCACATGAGTAA
ATGGTAGCAAATGGTAACGTTGAGAGCATTTCACTGCCGGAGGTAAGGCGCTTAATGTCAGGGGAACTTGACCGCTCAAG
CGCCTCTGAATGGGCGTTTGCAATAATTGATGATGATCACATTCGAGTAAGCGATCAGGTTGTTTGGAAAGTACTTCAAT
GCTTGGGGGGAGCAGATTTACCGATAACTGACAGGGAGTATTTATATGAAAAAGAAGACTTTAATTGCTGGCTCAACGAA
ATTGACTCACATGAGTAA