SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP011650
Component protein type	TagL
T6SS ID (Type)	T6SS00258 (Type i3)
Strain	Yersinia pseudotuberculosis YPIII
Replicon	chromosome
Sequence	Protein sequence (561 a.a.); Nucleotide sequence (1683 bp)
Reference	[1] Zhang W, Xu S, Li J, et al. Modulation of a thermoregulated type VI secretion system by AHL-dependent quorum sensing in Yersinia pseudotuberculosis. Arch Microbiol. 2011 May;193(5):351-63. doi: 10.1007/s00203-011-0680-2. Epub 2011 Feb 5. PMID: 21298257 [2] Zhang W, Wang Y, Song Y, et al. A type VI secretion system regulated by OmpR in Yersinia pseudotuberculosis functions to maintain intracellular pH homeostasis. Environ Microbiol. 2013 Feb;15(2):557-69. doi: 10.1111/1462-2920.12005. Epub 2012 Oct 24. PMID: 23094603 [3] Song L, Pan J, Yang Y, et al. Contact-independent killing mediated by a T6SS effector with intrinsic cell-entry properties. Nat Commun. 2021 Jan 18;12(1):423. doi: 10.1038/s41467-020-20726-8. PMID: 33462232

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPK_RS07400
Coordinate (Strand)	1619849..1621531 (-)
NCBI ID	WP_012104863.1
RefSeq	NC_010465
Uniprot ID	A0A0U1QV43_YERP3, A0A0H3B098_YERPY
KEGG ID	ypi:YpsIP31758_1355, ypy:YPK_1467
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
OmpA	PF00691.22	2e-17	441..539

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-9	MFKVKLNKT
Transmembrane helix	10-27	LSLLWLAAVGTLLVSVNF
Inside	28-31	KGNA
Transmembrane helix	32-54	LWNNVIFITFLALMVLAIWKHYA
Outside	55-560	LKPKHPQVPIPVPQETAEPVEQESTVILILGPYAEKWFSNPESPDSTRFSSHAVWILITNPAALQKRLTYIAEHHPSAQVLTFFPFLPDVYENAAVMMSKLTVWQNTFSTLSLRSPLPCVFAIYAQLSNERLSHNSDNAYWTGNINLANQQPIEITQALQSLSQRLELQDINHSGFATQRNVMAHNLFTWLNESGVTNLLQSIFSRTPLRLTDVILSDNGKGFIRHGAWSAWLEKKFGIIPSLASTLSLPPFPAVINDQKPAIKPRVKTPVIKPIPASRGWLWSVCFAAVLLASHMLNTLWQEKNRHAQFNQQMVPLDNINDFSAQYLARSITQLTDEGKTLTTCVNTFDVTRWGLSQCQTLLSQINRRIESYENIPVFSFAQKLPLFDSNSTKLKLNSQTNEMMMELLSLVEQNKGKKILIVGHSDNTGSSSMNMTLSEQRALALRDWLIKRSDITVDNFITKGMGASEPVATNHTEAGREQNRRVEVLILPTQDRTRMTEPKPL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9702	-	-

Protein sequence: 561 a.a.

						>T6CP011650 NC_010465:c1621531-1619849 [Yersinia pseudotuberculosis YPIII] [TagL]
MFKVKLNKTLSLLWLAAVGTLLVSVNFKGNALWNNVIFITFLALMVLAIWKHYALKPKHPQVPIPVPQETAEPVEQESTV
ILILGPYAEKWFSNPESPDSTRFSSHAVWILITNPAALQKRLTYIAEHHPSAQVLTFFPFLPDVYENAAVMMSKLTVWQN
TFSTLSLRSPLPCVFAIYAQLSNERLSHNSDNAYWTGNINLANQQPIEITQALQSLSQRLELQDINHSGFATQRNVMAHN
LFTWLNESGVTNLLQSIFSRTPLRLTDVILSDNGKGFIRHGAWSAWLEKKFGIIPSLASTLSLPPFPAVINDQKPAIKPR
VKTPVIKPIPASRGWLWSVCFAAVLLASHMLNTLWQEKNRHAQFNQQMVPLDNINDFSAQYLARSITQLTDEGKTLTTCV
NTFDVTRWGLSQCQTLLSQINRRIESYENIPVFSFAQKLPLFDSNSTKLKLNSQTNEMMMELLSLVEQNKGKKILIVGHS
DNTGSSSMNMTLSEQRALALRDWLIKRSDITVDNFITKGMGASEPVATNHTEAGREQNRRVEVLILPTQDRTRMTEPKPL
*					

Nucleotide sequence: 1683 bp

						>T6CP011650 NC_010465:c1621531-1619849 [Yersinia pseudotuberculosis YPIII] [TagL]
ATGTTTAAGGTTAAATTGAATAAAACGCTCTCTCTATTATGGCTGGCTGCGGTCGGCACCCTATTGGTGAGTGTGAATTT
TAAGGGAAATGCGTTATGGAATAACGTTATTTTCATTACTTTCCTGGCGCTGATGGTTCTTGCCATCTGGAAACATTACG
CCCTCAAACCCAAACACCCCCAAGTGCCGATTCCCGTCCCGCAGGAGACAGCGGAACCGGTAGAACAGGAAAGTACGGTT
ATTTTAATTCTCGGTCCCTATGCTGAGAAATGGTTTAGTAACCCAGAATCGCCAGACAGTACACGTTTTTCCAGCCATGC
GGTCTGGATATTAATCACGAATCCCGCCGCGCTGCAAAAACGCCTCACATATATTGCTGAACATCATCCATCCGCTCAGG
TATTGACCTTTTTCCCTTTCTTGCCGGATGTCTATGAGAATGCAGCAGTGATGATGTCAAAACTCACGGTGTGGCAGAAC
ACGTTTTCAACGTTATCACTGCGATCCCCCCTCCCCTGCGTATTTGCTATTTATGCGCAGTTGAGTAATGAACGCCTTAG
CCATAATTCTGATAACGCATATTGGACTGGAAATATTAATCTTGCGAACCAGCAGCCTATCGAAATTACGCAAGCCCTAC
AGTCACTGAGCCAAAGACTTGAGTTACAAGATATCAATCACTCAGGATTTGCAACTCAGCGTAATGTTATGGCCCATAAT
TTATTTACCTGGTTGAATGAATCGGGGGTGACAAACCTTCTGCAATCTATTTTCTCGCGCACCCCCTTACGGCTAACAGA
CGTCATTTTGTCGGATAATGGCAAAGGGTTCATCAGGCATGGTGCCTGGTCAGCCTGGCTGGAGAAAAAGTTTGGCATCA
TACCGAGCTTAGCCTCAACCTTGTCACTGCCACCTTTCCCTGCGGTGATCAATGACCAGAAGCCCGCAATAAAACCACGG
GTGAAAACACCGGTGATAAAACCTATCCCCGCATCCCGTGGGTGGTTATGGAGTGTGTGCTTCGCCGCGGTGCTATTAGC
CTCACATATGCTAAACACCCTGTGGCAAGAAAAAAACCGGCATGCGCAATTTAACCAACAGATGGTGCCGTTGGACAATA
TCAACGATTTTTCTGCTCAGTACCTGGCACGCAGCATCACCCAGTTAACCGATGAAGGTAAAACATTAACCACATGCGTT
AATACATTTGATGTGACACGTTGGGGGCTGTCTCAATGTCAGACGTTATTAAGCCAAATCAACCGCCGGATTGAATCCTA
TGAAAATATTCCTGTTTTCAGTTTTGCCCAAAAGCTCCCTCTGTTTGACTCAAACAGTACCAAGCTGAAACTGAACTCAC
AAACCAATGAAATGATGATGGAGTTGTTATCACTGGTCGAACAGAATAAAGGGAAAAAAATCTTAATTGTTGGCCATTCT
GATAACACGGGCAGTTCCTCCATGAACATGACACTTTCCGAACAGCGTGCGCTGGCCCTTCGCGATTGGTTAATTAAACG
CAGTGATATCACTGTCGATAATTTTATTACCAAGGGAATGGGGGCTTCAGAACCGGTTGCGACCAATCACACGGAAGCGG
GTCGAGAACAAAACAGACGCGTCGAAGTACTGATATTACCCACACAAGATAGAACAAGAATGACGGAGCCTAAACCTTTA
TGA					