SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002556
Component protein type	TssE
T6SS ID (Type)	T6SS00258 (Type i3)
Strain	Yersinia pseudotuberculosis YPIII
Replicon	chromosome
Sequence	Protein sequence (160 a.a.); Nucleotide sequence (480 bp)
Reference	[1] Zhang W, Xu S, Li J, et al. Modulation of a thermoregulated type VI secretion system by AHL-dependent quorum sensing in Yersinia pseudotuberculosis. Arch Microbiol. 2011 May;193(5):351-63. doi: 10.1007/s00203-011-0680-2. Epub 2011 Feb 5. PMID: 21298257 [2] Zhang W, Wang Y, Song Y, et al. A type VI secretion system regulated by OmpR in Yersinia pseudotuberculosis functions to maintain intracellular pH homeostasis. Environ Microbiol. 2013 Feb;15(2):557-69. doi: 10.1111/1462-2920.12005. Epub 2012 Oct 24. PMID: 23094603 [3] Song L, Pan J, Yang Y, et al. Contact-independent killing mediated by a T6SS effector with intrinsic cell-entry properties. Nat Commun. 2021 Jan 18;12(1):423. doi: 10.1038/s41467-020-20726-8. PMID: 33462232

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPK_RS07520
Coordinate (Strand)	1652892..1653371 (+)
NCBI ID	WP_002209686.1
RefSeq	NC_010465
Uniprot ID	A0A0E8XPJ3_9GAMM, Q668T2_YERPS, A0A0U1R3C0_YERP3, A0A0H2YI41_YERPN, A0A0H3B3J0_YERPY, A0A2U2H0H3_YERPE, A0A0T9JCT6_YERPU
KEGG ID	ypy:YPK_1490, ype:YPO2728, ypq:DJ40_3888, ypu:BZ21_1952, yps:YPTB2655, ypn:YPN_2219, ypc:BZ23_2236, ypr:BZ20_3554, ypo:BZ17_3983, ypi:YpsIP31758_1378
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
GPW_gp25	PF04965.16	1.9e-16	37..136

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-159	MEKKKQFLPALLERLLDDEPKKHIEPHDKFFYDSRTMRKLVQRNISEILNNANIEDRLDPQRHKWVATSVLNYGISPLVGLYAVPHNWATIERIIREAIIRFEPRIMAESLVVSQQLQARNGIIQFEIRGLIKWNPHPIDLCIEGAYDVETDNAELRAR

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9924	-	-

Protein sequence: 160 a.a.

						>T6CP002556 NC_010465:1652892-1653371 [Yersinia pseudotuberculosis YPIII] [TssE]
MEKKKQFLPALLERLLDDEPKKHIEPHDKFFYDSRTMRKLVQRNISEILNNANIEDRLDPQRHKWVATSVLNYGISPLVG
LYAVPHNWATIERIIREAIIRFEPRIMAESLVVSQQLQARNGIIQFEIRGLIKWNPHPIDLCIEGAYDVETDNAELRAR*					

Nucleotide sequence: 480 bp

						>T6CP002556 NC_010465:1652892-1653371 [Yersinia pseudotuberculosis YPIII] [TssE]
ATGGAAAAGAAAAAACAGTTTTTACCGGCGTTGTTAGAGCGTTTACTCGATGATGAGCCTAAAAAGCATATTGAGCCTCA
CGATAAATTCTTTTATGACTCACGGACGATGCGGAAATTGGTACAACGTAATATTTCAGAGATATTAAATAATGCCAATA
TTGAAGACCGGTTGGATCCGCAGCGTCATAAATGGGTAGCGACGTCGGTCTTAAACTATGGTATTTCACCCTTGGTTGGG
CTTTATGCCGTGCCGCATAATTGGGCGACTATTGAACGAATTATCAGAGAGGCCATTATCCGTTTTGAACCACGGATAAT
GGCGGAATCTCTCGTTGTCAGCCAGCAACTCCAGGCCAGGAATGGCATTATACAGTTTGAGATTAGGGGGTTAATCAAAT
GGAACCCGCACCCTATCGATCTTTGTATTGAAGGTGCCTATGACGTTGAGACGGATAACGCAGAGCTACGCGCCCGTTGA					