SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002550
Component protein type	TssD
T6SS ID (Type)	T6SS00258 (Type i3)
Strain	Yersinia pseudotuberculosis YPIII
Replicon	chromosome
Sequence	Protein sequence (162 a.a.); Nucleotide sequence (486 bp)
Reference	[1] Zhang W, Xu S, Li J, et al. Modulation of a thermoregulated type VI secretion system by AHL-dependent quorum sensing in Yersinia pseudotuberculosis. Arch Microbiol. 2011 May;193(5):351-63. doi: 10.1007/s00203-011-0680-2. Epub 2011 Feb 5. PMID: 21298257 [2] Zhang W, Wang Y, Song Y, et al. A type VI secretion system regulated by OmpR in Yersinia pseudotuberculosis functions to maintain intracellular pH homeostasis. Environ Microbiol. 2013 Feb;15(2):557-69. doi: 10.1111/1462-2920.12005. Epub 2012 Oct 24. PMID: 23094603 [3] Song L, Pan J, Yang Y, et al. Contact-independent killing mediated by a T6SS effector with intrinsic cell-entry properties. Nat Commun. 2021 Jan 18;12(1):423. doi: 10.1038/s41467-020-20726-8. PMID: 33462232

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPK_RS07475
Coordinate (Strand)	1639839..1640324 (+)
NCBI ID	WP_002211573.1
RefSeq	NC_010465
Uniprot ID	A0A0U1QUS9_YERP3, A0A0T9QX71_9GAMM, A0A5P8YHR4_YERPE, A0A0T9MKL1_YERPU
KEGG ID	ype:YPO2937, ysi:BF17_22805, ypw:CH59_3137, ypq:DJ40_3879, ypr:BZ20_3545, ypu:BZ21_1961, ypj:CH55_4142, ypl:CH46_2168, ypc:BZ23_2245, ypv:BZ15_594, ypi:YpsIP31758_1369
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_HCP	PF05638.14	1.6e-40	6..136

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-161	MAQDMFIKIDGIEGESLDANHKNEIQVLAWNWDVAQHSNMHSGSGGGSGKASVSDFCFAHYIDKASPNLLSYCLLGKHIKNVQFVLRKAGGDPLEYLTIKFTDVIITRVDMAGSLEDETRPREEIRFSFTKMTQDYVMQNAEGHKSGVISANYDVKANMHS

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9835	-	-

Protein sequence: 162 a.a.

						>T6CP002550 NC_010465:1639839-1640324 [Yersinia pseudotuberculosis YPIII] [TssD]
MAQDMFIKIDGIEGESLDANHKNEIQVLAWNWDVAQHSNMHSGSGGGSGKASVSDFCFAHYIDKASPNLLSYCLLGKHIK
NVQFVLRKAGGDPLEYLTIKFTDVIITRVDMAGSLEDETRPREEIRFSFTKMTQDYVMQNAEGHKSGVISANYDVKANMH
S*					

Nucleotide sequence: 486 bp

						>T6CP002550 NC_010465:1639839-1640324 [Yersinia pseudotuberculosis YPIII] [TssD]
ATGGCGCAAGATATGTTTATCAAAATTGATGGTATTGAAGGTGAGTCTTTGGATGCCAATCACAAAAACGAAATCCAGGT
ATTAGCTTGGAACTGGGATGTTGCTCAACACTCGAATATGCACAGCGGTTCAGGTGGCGGTTCAGGTAAAGCCTCTGTTT
CAGATTTTTGCTTCGCACATTATATCGATAAAGCTAGCCCGAACTTATTGAGCTATTGCTTGCTGGGTAAGCACATTAAA
AATGTTCAGTTCGTACTGCGTAAAGCCGGTGGCGATCCGCTGGAATATCTGACAATTAAATTTACCGATGTCATCATTAC
TCGTGTTGATATGGCGGGTTCTTTGGAAGATGAAACGCGTCCACGTGAAGAAATTCGTTTCTCATTCACTAAGATGACTC
AGGACTATGTTATGCAGAATGCTGAAGGCCATAAATCTGGCGTTATTTCTGCTAACTACGATGTTAAAGCTAACATGCAC
AGCTAA					