SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP002546
Component protein type	TssF
T6SS ID (Type)	T6SS00258 (Type i3)
Strain	Yersinia pseudotuberculosis YPIII
Replicon	chromosome
Sequence	Protein sequence (627 a.a.); Nucleotide sequence (1881 bp)
Reference	[1] Zhang W, Xu S, Li J, et al. Modulation of a thermoregulated type VI secretion system by AHL-dependent quorum sensing in Yersinia pseudotuberculosis. Arch Microbiol. 2011 May;193(5):351-63. doi: 10.1007/s00203-011-0680-2. Epub 2011 Feb 5. PMID: 21298257 [2] Zhang W, Wang Y, Song Y, et al. A type VI secretion system regulated by OmpR in Yersinia pseudotuberculosis functions to maintain intracellular pH homeostasis. Environ Microbiol. 2013 Feb;15(2):557-69. doi: 10.1111/1462-2920.12005. Epub 2012 Oct 24. PMID: 23094603 [3] Song L, Pan J, Yang Y, et al. Contact-independent killing mediated by a T6SS effector with intrinsic cell-entry properties. Nat Commun. 2021 Jan 18;12(1):423. doi: 10.1038/s41467-020-20726-8. PMID: 33462232

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPK_RS07430
Coordinate (Strand)	1626962..1628842 (-)
NCBI ID	WP_002211582.1
RefSeq	NC_010465
Uniprot ID	Q668R5_YERPS, A0A0U1QVU9_YERP3, A0A0H2Y9L2_YERPA, A0A0H2YHV0_YERPN, A0A0H3B1K7_YERPY, A0A5P8YHX9_YERPE, A0A3G5IXH3_YERPU
KEGG ID	ypa:YPA_2138, ypi:YpsIP31758_1361, yps:YPTB2672, ypn:YPN_1433, ype:YPO2947, ypy:YPK_1473
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	1.8e-215	2..623

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-626	VDHKFLDYYNKELTFMREMAQEFAEKHPKIAGRLGMHGIEVADPYVERLIESFCFLSARTQIKLDAEFPKFTQRLLDIVYPNYNSPTPSMGVIQLKPNLKEGDLTQGYCVPRGSSFFTHIAPGETTRCEFRSGQDVELWPLEIAEARLSSVPPDMPNLEKHRIAYHRLKGALRIKLKLVGDLTFSQLEGLDRLPIYIDGDERIVSHIFELLHASHVATIIRAGHGETAKDTVISDQPLSFEGLSPDQSLLPLSWNIFHGHNLVQEYFTCRQRFYFFTLTQLSQSLRDNHTGEAEIIILLDRLPQDLVSHVAPSRFLLACTPVINLFPKRMDKIEINRALNDFHVVPDRSRPLDYEVFAINKVFGQQAETSNEVVFNPLYQTRHCDNGNFGRYFSITRKARTGDSNKRKYDTRTPYASTEVFVSLVDQQEAPYGDSLRYLSIEAQVTNRDLPRLISASGNFELTMSDSAPVHGARFVFQPSAPRAPFAMDESAWRLIRQLSFNYLPLSDMSHSNGGESLRNMLRLFVNASDHESNTQIDALVGCKSEPVIRRLPGNGLLVYGRGICCTLTVDEEGFSGISPYLFGMIMENYVARHAAINVFTETELNSMQRGRVGHWKARPGRRGVL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9964	-	-

Protein sequence: 627 a.a.

						>T6CP002546 NC_010465:c1628842-1626962 [Yersinia pseudotuberculosis YPIII] [TssF]
VDHKFLDYYNKELTFMREMAQEFAEKHPKIAGRLGMHGIEVADPYVERLIESFCFLSARTQIKLDAEFPKFTQRLLDIVY
PNYNSPTPSMGVIQLKPNLKEGDLTQGYCVPRGSSFFTHIAPGETTRCEFRSGQDVELWPLEIAEARLSSVPPDMPNLEK
HRIAYHRLKGALRIKLKLVGDLTFSQLEGLDRLPIYIDGDERIVSHIFELLHASHVATIIRAGHGETAKDTVISDQPLSF
EGLSPDQSLLPLSWNIFHGHNLVQEYFTCRQRFYFFTLTQLSQSLRDNHTGEAEIIILLDRLPQDLVSHVAPSRFLLACT
PVINLFPKRMDKIEINRALNDFHVVPDRSRPLDYEVFAINKVFGQQAETSNEVVFNPLYQTRHCDNGNFGRYFSITRKAR
TGDSNKRKYDTRTPYASTEVFVSLVDQQEAPYGDSLRYLSIEAQVTNRDLPRLISASGNFELTMSDSAPVHGARFVFQPS
APRAPFAMDESAWRLIRQLSFNYLPLSDMSHSNGGESLRNMLRLFVNASDHESNTQIDALVGCKSEPVIRRLPGNGLLVY
GRGICCTLTVDEEGFSGISPYLFGMIMENYVARHAAINVFTETELNSMQRGRVGHWKARPGRRGVL*					

Nucleotide sequence: 1881 bp

						>T6CP002546 NC_010465:c1628842-1626962 [Yersinia pseudotuberculosis YPIII] [TssF]
GTGGATCACAAATTTCTTGACTATTACAATAAAGAACTCACTTTTATGCGCGAAATGGCGCAAGAGTTTGCTGAAAAACA
TCCCAAAATAGCAGGCCGCCTGGGCATGCATGGGATTGAAGTCGCCGATCCTTACGTTGAGCGGCTGATAGAGTCCTTCT
GTTTTTTATCTGCCAGAACGCAGATTAAGTTGGATGCTGAGTTTCCTAAATTTACTCAGCGTCTTTTGGACATTGTCTAT
CCCAATTACAATTCGCCAACCCCCTCAATGGGCGTGATCCAACTTAAGCCAAATCTGAAGGAAGGGGATTTGACTCAAGG
GTATTGTGTTCCTAGGGGCAGCTCGTTCTTCACCCATATCGCACCTGGCGAGACAACCCGCTGCGAGTTCCGTAGTGGTC
AGGATGTGGAGTTGTGGCCACTGGAAATAGCAGAAGCGCGTCTAAGCTCTGTTCCCCCGGATATGCCTAATCTGGAAAAG
CACCGCATTGCCTATCATCGGCTTAAAGGTGCCTTACGCATTAAATTAAAATTAGTGGGCGATCTCACTTTCTCACAATT
AGAAGGCCTTGATCGGCTGCCTATTTATATTGATGGTGATGAACGAATCGTTTCACATATCTTCGAGCTACTCCACGCCA
GCCATGTTGCTACCATCATACGGGCAGGCCACGGAGAGACAGCAAAAGACACCGTGATCAGTGATCAGCCACTGTCCTTT
GAAGGGCTAAGCCCCGACCAAAGCCTACTGCCGCTATCATGGAATATCTTTCACGGACATAATCTGGTGCAGGAGTATTT
TACCTGCCGCCAGCGTTTCTACTTTTTTACGCTGACACAGCTGTCGCAAAGTTTGCGCGATAATCATACCGGTGAAGCGG
AAATCATCATCCTACTGGACAGATTACCGCAGGATTTGGTGAGCCATGTTGCCCCCTCTCGCTTCCTGTTAGCCTGCACG
CCAGTGATTAATCTCTTTCCTAAACGCATGGATAAGATCGAAATTAACCGTGCATTGAACGATTTTCACGTAGTACCAGA
CCGCAGCCGCCCACTGGATTATGAAGTTTTCGCCATTAATAAAGTATTTGGTCAACAGGCGGAAACCAGCAACGAGGTGG
TATTTAACCCACTTTATCAAACCCGTCACTGTGATAATGGCAACTTTGGGCGTTATTTCTCCATCACCCGCAAGGCCAGA
ACGGGCGATAGCAACAAAAGAAAATATGACACCCGCACGCCTTACGCCAGTACCGAGGTCTTTGTCTCGTTGGTCGATCA
ACAAGAGGCGCCTTATGGCGATAGCTTGCGCTACCTCTCTATTGAAGCTCAGGTGACTAATCGGGATCTTCCGCGCCTGA
TTTCCGCCAGTGGTAATTTTGAATTGACCATGTCGGATTCAGCGCCCGTTCACGGTGCCCGGTTTGTGTTCCAACCCAGC
GCCCCACGCGCCCCCTTTGCCATGGATGAATCCGCCTGGCGTTTGATTCGCCAATTGAGCTTCAACTATCTGCCACTGTC
AGATATGTCCCACTCGAATGGGGGGGAGTCACTGCGCAATATGCTACGTCTGTTTGTTAACGCTTCAGACCATGAATCGA
ATACACAAATTGACGCGCTGGTGGGCTGTAAAAGCGAGCCGGTGATCCGCCGGTTACCCGGCAATGGCCTACTGGTTTAT
GGCCGGGGCATCTGCTGCACCTTGACTGTGGATGAAGAAGGATTCTCCGGTATCAGCCCTTATCTATTTGGCATGATCAT
GGAAAATTATGTGGCCCGCCATGCCGCAATAAATGTGTTTACCGAGACCGAGCTAAACTCCATGCAAAGAGGACGTGTCG
GTCACTGGAAAGCCCGCCCTGGCAGACGAGGAGTCCTGTAA					