Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   KCX70_RS23435 Genome accession   NZ_CP073105
Coordinates   998259..998444 (-) Length   61 a.a.
NCBI ID   WP_156716308.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain F2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 993259..1003444
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCX70_RS04765 (KCX70_04765) - 995240..996127 (-) 888 WP_212619453.1 ABC transporter ATP-binding protein -
  KCX70_RS04770 (KCX70_04770) - 996124..996957 (-) 834 WP_212619454.1 hypothetical protein -
  KCX70_RS04775 (KCX70_04775) - 996954..997754 (-) 801 WP_212619455.1 hypothetical protein -
  KCX70_RS04780 (KCX70_04780) - 997814..998239 (-) 426 WP_212619456.1 hypothetical protein -
  KCX70_RS23435 (KCX70_04785) pilA 998259..998444 (-) 186 WP_156716308.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  KCX70_RS04790 (KCX70_04790) pilB 998985..1000691 (+) 1707 WP_212619457.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KCX70_RS04795 (KCX70_04795) pilC 1000694..1001917 (+) 1224 WP_212619458.1 type II secretion system F family protein Machinery gene
  KCX70_RS04800 (KCX70_04800) pilD 1001914..1002780 (+) 867 WP_102846952.1 A24 family peptidase Machinery gene

Sequence


Protein


Download         Length: 61 a.a.        Molecular weight: 6553.68 Da        Isoelectric Point: 10.1002

>NTDB_id=557965 KCX70_RS23435 WP_156716308.1 998259..998444(-) (pilA) [Stutzerimonas stutzeri strain F2]
MKSIKKQKGFTLIELMIVVAIIGILAAVAIPQFQDYQAKGYDKSAQSDARNILTGGIANSN

Nucleotide


Download         Length: 186 bp        

>NTDB_id=557965 KCX70_RS23435 WP_156716308.1 998259..998444(-) (pilA) [Stutzerimonas stutzeri strain F2]
ATGAAGTCCATCAAGAAGCAAAAAGGTTTCACCCTGATCGAGCTGATGATCGTAGTTGCGATCATTGGCATCTTGGCAGC
AGTAGCCATCCCGCAGTTTCAAGACTACCAAGCCAAGGGCTACGACAAGTCGGCTCAGTCTGATGCCCGCAACATCCTGA
CCGGCGGCATCGCCAACTCCAACTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

69.388

80.328

0.557

  pilA Ralstonia pseudosolanacearum GMI1000

55.932

96.721

0.541

  pilA Pseudomonas aeruginosa PAK

73.81

68.852

0.508

  pilA Vibrio campbellii strain DS40M4

80.556

59.016

0.475

  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

56

81.967

0.459

  pilA Vibrio parahaemolyticus RIMD 2210633

56

81.967

0.459

  pilA Acinetobacter nosocomialis M2

84.848

54.098

0.459

  pilA2 Legionella pneumophila strain ERS1305867

87.097

50.82

0.443

  pilA2 Legionella pneumophila str. Paris

87.097

50.82

0.443

  pilA Vibrio cholerae C6706

77.143

57.377

0.443

  pilA Vibrio cholerae strain A1552

77.143

57.377

0.443

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

77.143

57.377

0.443

  pilA/pilA1 Eikenella corrodens VA1

77.143

57.377

0.443

  pilA/pilAII Pseudomonas stutzeri DSM 10701

55.102

80.328

0.443

  pilE Acinetobacter baumannii D1279779

56.522

75.41

0.426

  pilA/pilAI Pseudomonas stutzeri DSM 10701

78.788

54.098

0.426

  pilE Neisseria gonorrhoeae strain FA1090

78.788

54.098

0.426

  pilE Neisseria gonorrhoeae MS11

78.788

54.098

0.426

  comF Acinetobacter baylyi ADP1

55.556

73.77

0.41

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

75.758

54.098

0.41

  comP Acinetobacter baylyi ADP1

69.444

59.016

0.41

  pilX Neisseria meningitidis 8013

66.667

59.016

0.393

  pilL Neisseria gonorrhoeae MS11

66.667

59.016

0.393

  pilA Haemophilus influenzae 86-028NP

69.697

54.098

0.377

  pilA Haemophilus influenzae Rd KW20

69.697

54.098

0.377