Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   KCX70_RS04790 Genome accession   NZ_CP073105
Coordinates   998985..1000691 (+) Length   568 a.a.
NCBI ID   WP_212619457.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri strain F2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 993985..1005691
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KCX70_RS04765 (KCX70_04765) - 995240..996127 (-) 888 WP_212619453.1 ABC transporter ATP-binding protein -
  KCX70_RS04770 (KCX70_04770) - 996124..996957 (-) 834 WP_212619454.1 hypothetical protein -
  KCX70_RS04775 (KCX70_04775) - 996954..997754 (-) 801 WP_212619455.1 hypothetical protein -
  KCX70_RS04780 (KCX70_04780) - 997814..998239 (-) 426 WP_212619456.1 hypothetical protein -
  KCX70_RS23435 (KCX70_04785) pilA 998259..998444 (-) 186 WP_156716308.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  KCX70_RS04790 (KCX70_04790) pilB 998985..1000691 (+) 1707 WP_212619457.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KCX70_RS04795 (KCX70_04795) pilC 1000694..1001917 (+) 1224 WP_212619458.1 type II secretion system F family protein Machinery gene
  KCX70_RS04800 (KCX70_04800) pilD 1001914..1002780 (+) 867 WP_102846952.1 A24 family peptidase Machinery gene
  KCX70_RS04805 (KCX70_04805) coaE 1003014..1003622 (+) 609 WP_212619459.1 dephospho-CoA kinase -
  KCX70_RS04810 (KCX70_04810) yacG 1003619..1003816 (+) 198 WP_102846950.1 DNA gyrase inhibitor YacG -
  KCX70_RS04815 (KCX70_04815) - 1003848..1004540 (-) 693 WP_102846949.1 energy-coupling factor ABC transporter permease -
  KCX70_RS04820 (KCX70_04820) - 1004640..1005677 (-) 1038 WP_212619460.1 NADP(H)-dependent aldo-keto reductase -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62701.78 Da        Isoelectric Point: 5.4789

>NTDB_id=557942 KCX70_RS04790 WP_212619457.1 998985..1000691(+) (pilB) [Stutzerimonas stutzeri strain F2]
MNENAPLGGLARQLVLVGQLEEKIALQAQQQAQRNQVPLVSWLVQNKLVKSRAVAEVAGEQFGVPYFDLSTLDKDTQPKD
LVSEKLVRQHRVLPLQRRGNKLYVAISDPTNHQAITDIQFNTGLSVESVLVEDDKLGEAIEKLFESVSTSLDGMTDSDLD
ELDVEMSGGATERDEVGHSEADDAPVVRFVNKMLLDAIKRGSSDLHFEPYEKSYRVRFRTDGVLHEVARPPVQLVSRISA
RLKVMAALDIAERRKPQDGRIKMKVSKTKSIDFRVNSLPTLWGEKIVMRILDSSSAKMGIDALGYEEEQKELYLTALKQP
QGMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDIIMVGEIR
DLETAEIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCASCKKEVEVPREALLKEGF
PEDRIGTFQLYGPVGCDNCNGGYKGRVGIYEVVKITPALQRLIMEEGNSIDIAIQARKDGFNDLRISGLVKAMNGITSLE
EVNRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=557942 KCX70_RS04790 WP_212619457.1 998985..1000691(+) (pilB) [Stutzerimonas stutzeri strain F2]
ATGAACGAAAACGCTCCCCTCGGTGGTTTGGCTCGCCAGCTTGTATTGGTCGGCCAGCTCGAAGAAAAGATCGCGCTGCA
GGCCCAGCAGCAAGCTCAGCGCAATCAGGTGCCGCTAGTTTCCTGGCTGGTGCAGAACAAGCTGGTTAAAAGCCGGGCGG
TAGCGGAAGTCGCAGGCGAGCAGTTCGGTGTTCCTTATTTCGATCTGAGTACGCTGGATAAAGACACTCAGCCCAAGGAT
CTTGTCAGCGAAAAGCTGGTCCGCCAGCACCGTGTGCTGCCCCTACAGCGGCGTGGCAACAAGCTGTATGTCGCTATTTC
AGACCCCACTAACCATCAAGCCATCACTGATATCCAGTTCAATACAGGGCTCTCGGTCGAGTCGGTACTGGTTGAAGACG
ACAAGCTTGGTGAGGCCATTGAAAAGCTGTTTGAGTCGGTCAGTACCTCGCTGGATGGTATGACCGATTCGGATCTCGAT
GAGCTTGACGTCGAGATGTCAGGCGGAGCCACTGAAAGGGATGAAGTGGGCCATAGTGAGGCTGATGACGCCCCCGTTGT
TCGCTTCGTCAATAAGATGCTGCTGGATGCGATCAAGCGAGGCTCTTCCGACCTTCATTTCGAGCCTTATGAAAAGTCTT
ACCGCGTTCGCTTCCGCACCGACGGCGTGCTGCATGAAGTGGCGAGGCCGCCCGTGCAATTGGTCAGCCGGATATCGGCT
CGCTTAAAAGTTATGGCCGCGCTGGACATTGCTGAGCGACGCAAGCCGCAGGACGGCCGGATCAAGATGAAGGTTTCCAA
GACCAAGTCCATCGACTTCCGCGTCAACAGCTTGCCGACCCTGTGGGGCGAAAAGATCGTGATGCGCATCCTGGATTCCT
CCAGCGCCAAGATGGGCATCGATGCCTTGGGCTACGAGGAGGAGCAGAAAGAGCTCTACCTTACTGCGCTGAAGCAGCCG
CAAGGGATGATTCTCGTCACCGGACCGACCGGTTCGGGGAAGACGGTTTCCCTCTATACCGGTTTGAATATTCTCAACAC
GGTCGATGTGAATATTTCCACCGCCGAAGATCCGGTGGAGATCAACCTGGAGGGCATCAACCAGGTCAACGTCAATCCTC
GGCAGGGCATGGACTTTGCTCAGGCGCTGCGAGCTTTCCTGCGCCAGGATCCCGACATCATCATGGTGGGCGAGATCCGC
GACCTGGAGACCGCAGAGATTGCCGTCAAGGCAGCCCAGACCGGCCATATGGTGATGTCGACGTTGCACACCAACAGCGC
GGCGGAGACCCTGACCCGCCTGCGCAACATGGGCGTACCAGCTTTCAACATCGCAACCTCGGTCAACCTGATCATCGCCC
AGCGGCTGGCCCGAAAGCTCTGTGCAAGTTGCAAGAAAGAAGTCGAGGTGCCACGCGAGGCACTGCTGAAGGAGGGGTTC
CCCGAAGACCGCATCGGCACCTTCCAGCTTTATGGGCCGGTGGGCTGTGATAACTGCAACGGCGGTTACAAAGGCCGTGT
CGGTATTTATGAAGTGGTTAAAATCACGCCTGCCTTGCAGCGGCTTATCATGGAGGAAGGAAACTCCATCGATATCGCCA
TCCAGGCGCGCAAAGATGGTTTCAATGATCTGCGCATCTCCGGACTGGTGAAGGCCATGAATGGCATCACCAGCCTGGAA
GAAGTCAATCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(192-459)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60.142

98.944

0.595
  pilB Acinetobacter baylyi ADP1

59.22

99.296

0.588
  pilB Legionella pneumophila strain ERS1305867

54.13

100

0.542
  pilB Vibrio cholerae strain A1552

50.883

99.648

0.507
  pilF Neisseria gonorrhoeae MS11

50.357

98.592

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

50

96.479

0.482
  pilB Vibrio campbellii strain DS40M4

49.722

94.894

0.472
  pilF Thermus thermophilus HB27

38.652

99.296

0.384
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.786

100

0.379