Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   JWV26_RS24530 Genome accession   NZ_CP070505
Coordinates   3236962..3237141 (+) Length   59 a.a.
NCBI ID   WP_206417198.1    Uniprot ID   -
Organism   Ectopseudomonas toyotomiensis strain SM2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 3231962..3242141
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JWV26_RS14810 (JWV26_14810) pilD 3232189..3233055 (-) 867 WP_206417194.1 prepilin peptidase Machinery gene
  JWV26_RS14815 (JWV26_14815) pilC 3233059..3234279 (-) 1221 WP_206417195.1 type II secretion system F family protein Machinery gene
  JWV26_RS14820 (JWV26_14820) pilB 3234283..3235983 (-) 1701 WP_206417196.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JWV26_RS24525 (JWV26_14825) - 3236212..3236397 (+) 186 WP_003242736.1 type IV pilin protein -
  JWV26_RS14830 (JWV26_14830) - 3236409..3236864 (+) 456 WP_206417197.1 hypothetical protein -
  JWV26_RS24530 (JWV26_14835) pilA 3236962..3237141 (+) 180 WP_206417198.1 type IV pilin protein Machinery gene
  JWV26_RS14840 (JWV26_14840) - 3237376..3238017 (+) 642 WP_241201220.1 hypothetical protein -
  JWV26_RS14845 (JWV26_14845) - 3237987..3238859 (+) 873 WP_241201221.1 hypothetical protein -
  JWV26_RS14850 (JWV26_14850) - 3238856..3239767 (+) 912 WP_206417199.1 ABC transporter ATP-binding protein -
  JWV26_RS14855 (JWV26_14855) nadC 3239881..3240729 (-) 849 WP_206417200.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence


Protein


Download         Length: 59 a.a.        Molecular weight: 6465.60 Da        Isoelectric Point: 10.4690

>NTDB_id=540344 JWV26_RS24530 WP_206417198.1 3236962..3237141(+) (pilA) [Ectopseudomonas toyotomiensis strain SM2]
MRSKNSGFTLIELMIVVAIIGILAAIAIPQFNEYRAKANDANAQADVKNSLWIMASSRK

Nucleotide


Download         Length: 180 bp        

>NTDB_id=540344 JWV26_RS24530 WP_206417198.1 3236962..3237141(+) (pilA) [Ectopseudomonas toyotomiensis strain SM2]
ATGCGTTCTAAAAATTCCGGCTTCACATTGATCGAATTGATGATCGTGGTCGCCATTATCGGCATTCTTGCCGCCATCGC
CATTCCTCAGTTCAATGAGTATCGCGCCAAGGCGAACGACGCCAATGCCCAAGCCGACGTCAAGAACTCTTTGTGGATCA
TGGCTTCCTCGCGGAAGTGA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila strain ERS1305867

64.583

81.356

0.525

  pilA2 Legionella pneumophila str. Paris

64.583

81.356

0.525

  pilA Pseudomonas aeruginosa PAK

73.171

69.492

0.508

  pilA Ralstonia pseudosolanacearum GMI1000

50.847

100

0.508

  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

50

98.305

0.492

  pilA/pilAI Pseudomonas stutzeri DSM 10701

65.854

69.492

0.458

  comF Acinetobacter baylyi ADP1

57.447

79.661

0.458

  pilA Acinetobacter nosocomialis M2

72.973

62.712

0.458

  pilA Acinetobacter baumannii strain A118

84.375

54.237

0.458

  comE Acinetobacter baylyi ADP1

54.167

81.356

0.441

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

60.465

72.881

0.441

  pilA/pilAII Pseudomonas stutzeri DSM 10701

55.319

79.661

0.441

  pilA Vibrio parahaemolyticus RIMD 2210633

55.319

79.661

0.441

  pilA Vibrio campbellii strain DS40M4

70.27

62.712

0.441

  pilA/pilA1 Eikenella corrodens VA1

63.158

64.407

0.407

  pilE Acinetobacter baumannii D1279779

55.814

72.881

0.407

  comP Acinetobacter baylyi ADP1

75

54.237

0.407

  pilA Vibrio cholerae C6706

68.571

59.322

0.407

  pilA Vibrio cholerae strain A1552

68.571

59.322

0.407

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

68.571

59.322

0.407

  pilA Haemophilus influenzae 86-028NP

71.875

54.237

0.39

  pilA Haemophilus influenzae Rd KW20

60.526

64.407

0.39

  pilE Neisseria gonorrhoeae MS11

60.526

64.407

0.39

  pilE Neisseria gonorrhoeae strain FA1090

58.974

66.102

0.39

  pilA Glaesserella parasuis strain SC1401

70.968

52.542

0.373