Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   JWV26_RS14820 Genome accession   NZ_CP070505
Coordinates   3234283..3235983 (-) Length   566 a.a.
NCBI ID   WP_206417196.1    Uniprot ID   -
Organism   Ectopseudomonas toyotomiensis strain SM2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3229283..3240983
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  JWV26_RS14790 (JWV26_14790) - 3230103..3230792 (+) 690 WP_072423154.1 energy-coupling factor ABC transporter permease -
  JWV26_RS14795 (JWV26_14795) - 3230900..3231115 (+) 216 WP_206417192.1 hypothetical protein -
  JWV26_RS14800 (JWV26_14800) yacG 3231212..3231406 (-) 195 WP_004425006.1 DNA gyrase inhibitor YacG -
  JWV26_RS14805 (JWV26_14805) coaE 3231403..3232011 (-) 609 WP_206417193.1 dephospho-CoA kinase -
  JWV26_RS14810 (JWV26_14810) pilD 3232189..3233055 (-) 867 WP_206417194.1 prepilin peptidase Machinery gene
  JWV26_RS14815 (JWV26_14815) pilC 3233059..3234279 (-) 1221 WP_206417195.1 type II secretion system F family protein Machinery gene
  JWV26_RS14820 (JWV26_14820) pilB 3234283..3235983 (-) 1701 WP_206417196.1 type IV-A pilus assembly ATPase PilB Machinery gene
  JWV26_RS24525 (JWV26_14825) - 3236212..3236397 (+) 186 WP_003242736.1 type IV pilin protein -
  JWV26_RS14830 (JWV26_14830) - 3236409..3236864 (+) 456 WP_206417197.1 hypothetical protein -
  JWV26_RS24530 (JWV26_14835) pilA 3236962..3237141 (+) 180 WP_206417198.1 type IV pilin protein Machinery gene
  JWV26_RS14840 (JWV26_14840) - 3237376..3238017 (+) 642 WP_241201220.1 hypothetical protein -
  JWV26_RS14845 (JWV26_14845) - 3237987..3238859 (+) 873 WP_241201221.1 hypothetical protein -
  JWV26_RS14850 (JWV26_14850) - 3238856..3239767 (+) 912 WP_206417199.1 ABC transporter ATP-binding protein -
  JWV26_RS14855 (JWV26_14855) nadC 3239881..3240729 (-) 849 WP_206417200.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62078.01 Da        Isoelectric Point: 5.4511

>NTDB_id=540331 JWV26_RS14820 WP_206417196.1 3234283..3235983(-) (pilB) [Ectopseudomonas toyotomiensis strain SM2]
MSDTAQLTGLARQLVNASLIDERTASQAFAQALRNKIPLVSYLVQNKLASSRAVAEIAAEQFGVPYFDLSAVDKEHQPKD
LVSEKLCRQHRVLPLYRRGNKLFIALSDPTNHQALTDVQFSTGISTEALLVEDDKLGDALDKFFDSGTSGMEDLADVDLD
GLDVEAVSDEADDAGAVNDSDDAPVVRFINKMLLDAIKGGSSDLHFEPYEKAYRVRFRTDGMLHEVARPPIQLASRLSAR
LKVMAQLDISERRKPQDGRIKMKVSKTKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEEQKELYMTALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPKHGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSCKKEVALPREALLKEGFPE
SKIGSFKLFAPNGCDNCKGGYKGRVGIYEVVKITPSLQRIIMEEGNSIEIAAQAKADGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=540331 JWV26_RS14820 WP_206417196.1 3234283..3235983(-) (pilB) [Ectopseudomonas toyotomiensis strain SM2]
ATGAGCGATACCGCCCAACTCACCGGCCTCGCCCGCCAGCTGGTCAATGCCAGCCTTATCGACGAACGCACCGCCAGCCA
GGCCTTTGCCCAGGCACTGCGCAACAAGATTCCGCTGGTGTCCTATCTGGTACAGAACAAGTTGGCCTCGAGCCGTGCCG
TGGCCGAGATCGCCGCCGAGCAGTTCGGCGTGCCCTACTTCGACCTCAGCGCAGTAGACAAGGAGCACCAACCGAAGGAT
CTCGTCAGCGAGAAACTGTGCCGCCAGCACCGCGTGTTGCCGCTCTACCGTCGCGGCAACAAGCTGTTCATCGCTCTGTC
CGACCCGACCAATCACCAGGCCCTTACCGATGTTCAGTTCAGCACCGGCATCAGTACCGAAGCCCTGCTGGTCGAAGATG
ACAAGCTGGGCGACGCACTGGACAAGTTTTTCGATTCCGGTACAAGCGGCATGGAAGATCTGGCTGACGTCGATCTGGAC
GGACTGGATGTCGAAGCCGTCAGCGATGAAGCGGACGATGCTGGCGCGGTTAACGATTCTGACGATGCGCCTGTAGTGCG
CTTCATCAACAAGATGCTGCTGGACGCCATCAAGGGTGGCTCATCCGACCTGCACTTCGAGCCCTACGAAAAAGCCTACC
GTGTACGTTTTCGCACCGACGGCATGCTGCATGAGGTAGCCCGCCCGCCCATCCAGCTAGCCAGCAGACTTTCCGCCCGC
CTCAAGGTCATGGCCCAACTGGATATCTCCGAGCGGCGCAAGCCGCAGGACGGCCGGATCAAGATGAAGGTATCCAAGAC
CAAAGCCATCGACTTTCGCGTCAACACCCTGCCCACCCTCTGGGGCGAGAAAATCGTGATGCGGATTCTCGACCCCACCA
GTGCGCAGATGGGTATCGATGCACTCGGCTATGAAGAGGAACAGAAAGAGCTGTACATGACAGCTCTGAAGCAGCCGCAA
GGCATGATTCTGGTGACCGGCCCAACCGGCTCAGGCAAAACGGTATCGCTTTATACCGGCCTGAATATTCTCAACACCAC
GGACATCAATATTTCCACCGCGGAAGATCCGGTGGAAATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGAAAC
ACGGCATGGATTTCGCCCAGGCCCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTGGGTGAGATCCGCGAC
CTGGAAACCGCCGAAATCGCCATCAAGGCCGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAATAGCGCTGC
GGAAACCCTGACACGCCTGCGCAACATGGGCGTGCCGGCATTCAACATCGCCACCTCGGTCAACCTGATTATCGCCCAGC
GCCTGGCGCGCAAACTCTGCAGTTGCAAGAAGGAAGTCGCACTACCGCGCGAGGCCTTGCTGAAAGAAGGCTTTCCTGAA
AGCAAGATTGGCAGCTTCAAGCTTTTTGCCCCCAACGGCTGTGACAATTGCAAAGGCGGCTATAAAGGAAGGGTGGGGAT
TTATGAAGTAGTTAAAATCACGCCCTCCCTGCAACGCATTATCATGGAGGAAGGCAACTCCATCGAAATCGCCGCGCAGG
CGAAAGCCGACGGCTTCAATGACCTCCGAACTTCCGGCCTGCTCAAGGCGATGCAGGGCATCACCAGCCTGGAAGAAGTC
AACCGTGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.86

100

0.606
  pilB Acinetobacter baylyi ADP1

59.574

99.647

0.594
  pilB Legionella pneumophila strain ERS1305867

54.24

100

0.542
  pilB Vibrio cholerae strain A1552

50.438

100

0.509
  pilF Neisseria gonorrhoeae MS11

49.647

100

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

51.595

94.17

0.486
  pilB Vibrio campbellii strain DS40M4

48.845

99.47

0.486
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.228

93.11

0.375
  pilF Thermus thermophilus HB27

37.478

99.47

0.373