Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   A458_RS45270 Genome accession   NC_018028
Coordinates   3556402..3556587 (+) Length   61 a.a.
NCBI ID   WP_014821363.1    Uniprot ID   I4CWR3
Organism   Stutzerimonas stutzeri CCUG 29243     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 3551402..3561587
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A458_RS38240 (A458_16455) coaE 3551526..3552134 (-) 609 WP_014821359.1 dephospho-CoA kinase -
  A458_RS38245 (A458_16460) pilD 3552266..3553129 (-) 864 WP_014821360.1 prepilin peptidase Machinery gene
  A458_RS38250 (A458_16465) pilC 3553135..3554355 (-) 1221 WP_014821361.1 type II secretion system F family protein Machinery gene
  A458_RS38255 (A458_16470) pilB 3554358..3556061 (-) 1704 WP_014821362.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A458_RS45270 (A458_16475) pilA 3556402..3556587 (+) 186 WP_014821363.1 type IV pilin protein Machinery gene
  A458_RS38265 (A458_16480) - 3556608..3557042 (+) 435 WP_014821364.1 hypothetical protein -
  A458_RS38270 (A458_16485) - 3557108..3557908 (+) 801 WP_014821365.1 ABC transporter permease subunit -
  A458_RS38275 (A458_16490) - 3557905..3558738 (+) 834 WP_148273160.1 hypothetical protein -
  A458_RS38280 (A458_16495) - 3558735..3559619 (+) 885 WP_014821367.1 ABC transporter ATP-binding protein -
  A458_RS38285 (A458_16500) - 3559635..3560678 (+) 1044 WP_014821368.1 acyltransferase family protein -

Sequence


Protein


Download         Length: 61 a.a.        Molecular weight: 6585.70 Da        Isoelectric Point: 9.7375

>NTDB_id=51783 A458_RS45270 WP_014821363.1 3556402..3556587(+) (pilA) [Stutzerimonas stutzeri CCUG 29243]
MKSIKQQKGFTLIELMIVVAIIGILAAVAIPQFQDYQAKGYDKSAQSDARNIMTGAIANSN

Nucleotide


Download         Length: 186 bp        

>NTDB_id=51783 A458_RS45270 WP_014821363.1 3556402..3556587(+) (pilA) [Stutzerimonas stutzeri CCUG 29243]
ATGAAGTCCATCAAGCAGCAAAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGC
AGTCGCCATTCCACAGTTCCAGGATTACCAGGCTAAGGGCTATGACAAGTCGGCTCAGTCCGATGCGCGCAATATCATGA
CCGGCGCCATCGCCAACTCCAACTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB I4CWR3

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

69.388

80.328

0.557

  pilA Ralstonia pseudosolanacearum GMI1000

55.932

96.721

0.541

  pilA Pseudomonas aeruginosa PAK

73.81

68.852

0.508

  pilA Vibrio campbellii strain DS40M4

73.81

68.852

0.508

  pilV Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

57.407

88.525

0.508

  pilA Vibrio cholerae O1 biovar El Tor strain E7946

80

57.377

0.459

  pilA Vibrio cholerae strain A1552

80

57.377

0.459

  pilA Vibrio cholerae C6706

80

57.377

0.459

  pilA Vibrio parahaemolyticus RIMD 2210633

53.846

85.246

0.459

  pilA Acinetobacter nosocomialis M2

84.848

54.098

0.459

  pilA Haemophilus influenzae 86-028NP

64.286

68.852

0.443

  pilA2 Legionella pneumophila strain ERS1305867

87.097

50.82

0.443

  pilA2 Legionella pneumophila str. Paris

87.097

50.82

0.443

  pilA/pilA1 Eikenella corrodens VA1

77.143

57.377

0.443

  pilA/pilAII Pseudomonas stutzeri DSM 10701

55.102

80.328

0.443

  pilE Acinetobacter baumannii D1279779

56.522

75.41

0.426

  pilA/pilAI Pseudomonas stutzeri DSM 10701

78.788

54.098

0.426

  pilE Neisseria gonorrhoeae strain FA1090

78.788

54.098

0.426

  pilE Neisseria gonorrhoeae MS11

78.788

54.098

0.426

  comF Acinetobacter baylyi ADP1

55.556

73.77

0.41

  comP Acinetobacter baylyi ADP1

75.758

54.098

0.41

  pilE Neisseria elongata subsp. glycolytica ATCC 29315

75.758

54.098

0.41

  pilL Neisseria gonorrhoeae MS11

66.667

59.016

0.393

  pilX Neisseria meningitidis 8013

66.667

59.016

0.393

  pilA Haemophilus influenzae Rd KW20

69.697

54.098

0.377


Multiple sequence alignment