Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A458_RS38255 Genome accession   NC_018028
Coordinates   3554358..3556061 (-) Length   567 a.a.
NCBI ID   WP_014821362.1    Uniprot ID   -
Organism   Stutzerimonas stutzeri CCUG 29243     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3549358..3561061
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A458_RS38230 (A458_16440) - 3549541..3550578 (+) 1038 WP_014821356.1 NADP(H)-dependent aldo-keto reductase -
  A458_RS38235 (A458_16445) - 3550641..3551321 (+) 681 WP_014821357.1 energy-coupling factor ABC transporter permease -
  A458_RS44570 (A458_16450) yacG 3551332..3551529 (-) 198 WP_014821358.1 DNA gyrase inhibitor YacG -
  A458_RS38240 (A458_16455) coaE 3551526..3552134 (-) 609 WP_014821359.1 dephospho-CoA kinase -
  A458_RS38245 (A458_16460) pilD 3552266..3553129 (-) 864 WP_014821360.1 prepilin peptidase Machinery gene
  A458_RS38250 (A458_16465) pilC 3553135..3554355 (-) 1221 WP_014821361.1 type II secretion system F family protein Machinery gene
  A458_RS38255 (A458_16470) pilB 3554358..3556061 (-) 1704 WP_014821362.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A458_RS45270 (A458_16475) pilA 3556402..3556587 (+) 186 WP_014821363.1 type IV pilin protein Machinery gene
  A458_RS38265 (A458_16480) - 3556608..3557042 (+) 435 WP_014821364.1 hypothetical protein -
  A458_RS38270 (A458_16485) - 3557108..3557908 (+) 801 WP_014821365.1 ABC transporter permease subunit -
  A458_RS38275 (A458_16490) - 3557905..3558738 (+) 834 WP_148273160.1 hypothetical protein -
  A458_RS38280 (A458_16495) - 3558735..3559619 (+) 885 WP_014821367.1 ABC transporter ATP-binding protein -
  A458_RS38285 (A458_16500) - 3559635..3560678 (+) 1044 WP_014821368.1 acyltransferase family protein -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62100.98 Da        Isoelectric Point: 5.7969

>NTDB_id=51773 A458_RS38255 WP_014821362.1 3554358..3556061(-) (pilB) [Stutzerimonas stutzeri CCUG 29243]
MNENAPLGGLARQLVAAGQLEENVAYQAQQQALRNQVPLVTWLVQSKLVKAGALVSIAGEQFGLPFFDLSTLDKETQPKE
LVSEKLVRQHRALPLQRRGNRLYVAVSDPTNHQAISDIQFSTGLSVEAVLVEDGKLSEAIEKYYESSASSLDGMSDSDLD
DLVVGSSPDGDESAADHNDADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKSYRVRFRTDGMLHEVARPPVQLVSRISAR
LKVMASLDIAERRKPQDGRIKMKVSKTKSIDFRVNTLPTLWGEKIVMRILDSASAKMGIDALGYEEGQKELYLTALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCPSCKAEVEVPRDVLLREGFP
EARIGTFKIYAPVGCDSCKAGYKGRVGIYEVVKITPALQRLIMEEGNSIDIALQARRDGFNDLRTSGLVKAMNGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=51773 A458_RS38255 WP_014821362.1 3554358..3556061(-) (pilB) [Stutzerimonas stutzeri CCUG 29243]
ATGAACGAAAATGCGCCCCTCGGTGGTTTGGCTCGCCAGCTGGTTGCGGCTGGGCAGCTTGAGGAAAATGTTGCTTACCA
AGCTCAACAGCAGGCCTTGCGCAACCAAGTACCGCTGGTGACTTGGCTGGTGCAGAGCAAGCTGGTGAAGGCGGGCGCGT
TGGTCAGCATCGCTGGCGAGCAATTCGGCCTGCCATTCTTCGACCTCTCCACTCTGGATAAAGAAACTCAGCCTAAGGAG
TTGGTGAGCGAAAAGCTGGTGCGTCAGCATCGCGCGCTTCCTCTTCAGCGTCGCGGCAATCGGCTTTACGTGGCGGTGTC
TGATCCTACTAATCATCAGGCTATAAGCGATATTCAATTTAGTACCGGCCTATCTGTCGAGGCGGTGCTCGTCGAGGATG
GCAAGTTAAGCGAGGCTATAGAGAAATACTATGAGTCCTCTGCCTCCTCTTTGGATGGCATGAGTGATTCCGATCTTGAC
GATCTTGTTGTCGGGAGTAGTCCTGATGGCGATGAGAGCGCAGCTGATCACAATGACGCCGATGACGCGCCCGTCGTTCG
CTTCGTAAATAAAATGCTTCTTGATGCGATTAGAGGCGGCTCTTCCGACTTGCACTTCGAGCCATATGAAAAAAGCTACC
GCGTTCGGTTTCGTACCGATGGCATGCTGCACGAGGTCGCTAGGCCGCCGGTGCAGCTAGTCAGTCGAATCTCGGCGCGT
TTGAAAGTGATGGCCAGTCTCGATATCGCTGAGCGGCGAAAACCCCAAGATGGGCGAATCAAGATGAAGGTGTCCAAAAC
GAAGTCGATCGATTTCCGCGTCAATACGCTACCTACGCTCTGGGGCGAGAAGATTGTCATGCGGATTTTGGACTCAGCCA
GCGCGAAAATGGGTATTGACGCCCTGGGCTACGAAGAAGGCCAGAAAGAGCTCTATCTGACCGCGCTGAAGCAACCGCAG
GGTATGATATTGGTTACTGGGCCGACGGGCTCGGGTAAGACCGTTTCGTTGTATACCGGTCTGAATATTCTGAATACGGT
TGATGTAAATATTTCTACGGCAGAAGACCCCGTCGAGATAAATCTGGAGGGAATCAACCAAGTCAATGTTAATCCCAGGC
AGGGGATGGACTTCGCTCAAGCTCTGCGCGCGTTCTTGCGTCAGGATCCAGATGTCATCATGGTAGGGGAGATCCGGGAT
TTGGAGACTGCCGAAATTGCCATCAAGGCTGCTCAAACCGGTCACATGGTGATGTCCACATTGCACACCAATAGTGCCGC
GGAGACGCTGACGCGACTTCGAAACATGGGCGTGCCCGCCTTCAATATCGCTACTTCCGTGAACCTGATCATTGCCCAAC
GGTTGGCCCGCAAGCTATGTCCATCTTGCAAGGCTGAGGTGGAGGTGCCGCGTGACGTGCTCTTGCGGGAGGGTTTTCCT
GAGGCGAGGATCGGAACGTTCAAGATATACGCTCCGGTTGGATGCGACAGCTGTAAGGCTGGCTACAAGGGTCGTGTCGG
TATTTATGAAGTAGTTAAAATCACGCCGGCCCTGCAGCGGCTTATCATGGAGGAAGGTAATTCCATCGATATCGCCTTGC
AGGCGCGCAGAGATGGATTTAATGACCTGCGCACTTCGGGGCTGGTTAAGGCCATGAACGGCGTCACCAGTCTGGAAGAA
GTCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(191-458)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-148)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.467

98.942

0.578
  pilB Acinetobacter baylyi ADP1

57.345

99.647

0.571
  pilB Legionella pneumophila strain ERS1305867

53.765

100

0.541
  pilB Vibrio parahaemolyticus RIMD 2210633

52.102

96.473

0.503
  pilB Vibrio cholerae strain A1552

51.927

96.12

0.499
  pilB Vibrio campbellii strain DS40M4

50.823

96.473

0.49
  pilF Neisseria gonorrhoeae MS11

48.85

99.647

0.487
  pilF Thermus thermophilus HB27

38.366

99.295

0.381
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.943

99.471

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

39.615

91.711

0.363

Multiple sequence alignment