Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   A458_RS38250 Genome accession   NC_018028
Coordinates   3553135..3554355 (-) Length   406 a.a.
NCBI ID   WP_014821361.1    Uniprot ID   I4CWR1
Organism   Stutzerimonas stutzeri CCUG 29243     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3548135..3559355
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A458_RS38220 (A458_16430) rpsI 3548274..3548666 (-) 393 WP_003302797.1 30S ribosomal protein S9 -
  A458_RS38225 (A458_16435) rplM 3548681..3549109 (-) 429 WP_014821355.1 50S ribosomal protein L13 -
  A458_RS38230 (A458_16440) - 3549541..3550578 (+) 1038 WP_014821356.1 NADP(H)-dependent aldo-keto reductase -
  A458_RS38235 (A458_16445) - 3550641..3551321 (+) 681 WP_014821357.1 energy-coupling factor ABC transporter permease -
  A458_RS44570 (A458_16450) yacG 3551332..3551529 (-) 198 WP_014821358.1 DNA gyrase inhibitor YacG -
  A458_RS38240 (A458_16455) coaE 3551526..3552134 (-) 609 WP_014821359.1 dephospho-CoA kinase -
  A458_RS38245 (A458_16460) pilD 3552266..3553129 (-) 864 WP_014821360.1 prepilin peptidase Machinery gene
  A458_RS38250 (A458_16465) pilC 3553135..3554355 (-) 1221 WP_014821361.1 type II secretion system F family protein Machinery gene
  A458_RS38255 (A458_16470) pilB 3554358..3556061 (-) 1704 WP_014821362.1 type IV-A pilus assembly ATPase PilB Machinery gene
  A458_RS45270 (A458_16475) pilA 3556402..3556587 (+) 186 WP_014821363.1 type IV pilin protein Machinery gene
  A458_RS38265 (A458_16480) - 3556608..3557042 (+) 435 WP_014821364.1 hypothetical protein -
  A458_RS38270 (A458_16485) - 3557108..3557908 (+) 801 WP_014821365.1 ABC transporter permease subunit -
  A458_RS38275 (A458_16490) - 3557905..3558738 (+) 834 WP_148273160.1 hypothetical protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 44248.19 Da        Isoelectric Point: 9.8733

>NTDB_id=51772 A458_RS38250 WP_014821361.1 3553135..3554355(-) (pilC) [Stutzerimonas stutzeri CCUG 29243]
MAEKALKTSVFTWEGTDRKGGKVKGELAGQSPSLIKAQLRKQGITPVKVRKKAIPLFGGGKKITPMDIALFTRQMATMMK
AGVPLLQSFDIIGEGFENQNMRKLVDEIKQEVAAGNSYAASLRKKPRYFDDLYCNLVESGEQSGSLETLLDRIATYKEKT
EALKAKIKKAMTYPIAVILVAVIVSAILLIKVVPQFESVFHGFGAELPAFTMMVVGLSRWLQDWWYMTFGAFVAVAFVFK
HLHKRSEKLRDGLDRALLKMPIVGDILYKSALARYARTLSTTFAAGVPLVDALGSVAGAAGNVVFRNAVMKIRDEVSSGV
QLNFSMRGTGVFPAMAVQMAAIGEESGALDEMLDKLASFYEAEVDNMVDNLTTLMEPLIMAVLGVLVGGLIIAMYLPIFQ
LGGVIG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=51772 A458_RS38250 WP_014821361.1 3553135..3554355(-) (pilC) [Stutzerimonas stutzeri CCUG 29243]
ATGGCGGAAAAAGCGTTAAAAACCAGCGTGTTTACCTGGGAAGGCACTGATCGAAAAGGTGGGAAAGTCAAGGGGGAGCT
GGCAGGTCAGAGCCCATCTTTGATCAAGGCGCAACTTCGTAAGCAGGGCATTACACCTGTCAAAGTTCGCAAAAAAGCTA
TTCCGCTTTTTGGTGGGGGCAAAAAGATCACGCCAATGGACATTGCTCTGTTCACTCGGCAAATGGCGACCATGATGAAG
GCTGGCGTGCCGCTGTTACAGTCGTTTGACATCATTGGCGAAGGTTTCGAAAACCAAAATATGCGCAAGTTGGTTGACGA
AATTAAACAGGAAGTGGCTGCGGGTAACAGTTATGCCGCGTCCCTGCGCAAAAAACCTCGGTACTTCGACGATCTGTATT
GCAACTTGGTGGAGTCTGGCGAGCAGTCTGGTTCGTTGGAAACGTTGCTGGATCGAATAGCGACTTACAAGGAAAAGACC
GAGGCATTAAAGGCTAAGATCAAGAAAGCGATGACTTATCCTATCGCAGTGATTCTTGTGGCGGTTATTGTTTCTGCAAT
ACTGTTGATAAAAGTAGTTCCGCAGTTTGAAAGCGTTTTCCATGGATTTGGTGCTGAGCTGCCAGCATTTACTATGATGG
TAGTTGGCCTGTCTCGTTGGTTGCAAGACTGGTGGTACATGACGTTTGGTGCGTTCGTCGCCGTCGCGTTCGTGTTTAAG
CATCTTCATAAGCGCTCCGAAAAGCTCCGAGACGGCTTGGATCGAGCGCTGCTTAAAATGCCTATCGTGGGGGACATTCT
ATATAAGTCGGCGTTGGCTCGATATGCGCGTACGCTTTCGACAACATTTGCTGCGGGTGTGCCTCTAGTCGATGCCCTCG
GCTCCGTAGCTGGGGCGGCGGGTAACGTCGTGTTTCGTAATGCGGTCATGAAGATCCGCGACGAGGTGTCTTCAGGGGTG
CAGTTGAATTTCTCAATGCGCGGCACTGGAGTATTCCCAGCGATGGCTGTCCAGATGGCTGCTATCGGTGAGGAGTCTGG
AGCGCTAGATGAAATGCTAGATAAGCTTGCAAGCTTCTACGAGGCGGAAGTCGACAATATGGTCGACAACCTTACAACGC
TAATGGAGCCTCTGATCATGGCCGTGCTCGGTGTGCTGGTAGGTGGTTTGATTATTGCTATGTACTTGCCCATCTTCCAG
CTAGGCGGAGTTATCGGGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB I4CWR1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.012

99.754

0.788
  pilC Acinetobacter baylyi ADP1

59.852

100

0.599
  pilC Acinetobacter baumannii D1279779

59.753

99.754

0.596
  pilC Legionella pneumophila strain ERS1305867

53.283

97.537

0.52
  pilG Neisseria gonorrhoeae MS11

44.279

99.015

0.438
  pilG Neisseria meningitidis 44/76-A

44.169

99.261

0.438
  pilC Vibrio cholerae strain A1552

42.365

100

0.424
  pilC Vibrio campbellii strain DS40M4

40.796

99.015

0.404

Multiple sequence alignment