Detailed information NTG0176837

Bioinformatically predicted

Overview

• Name: pilA2
• Type: Machinery gene
• Locus tag: A4W93_RS01200
• Genome accession: NZ_CP015118
• Coordinates: 268234..268656 (+)
• Length: 140 a.a.
• NCBI ID: WP_085748872.1
• Uniprot ID: A0A1W6L2Y2
• Organism: Piscinibacter gummiphilus strain NS21
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 263234..273656

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A4W93_RS01175 (A4W93_01155)	-	263313..264065 (+)	753	WP_085748867.1	TerC family protein	-
A4W93_RS01180 (A4W93_01160)	-	264124..265377 (+)	1254	WP_085748868.1	serine/threonine-protein kinase	-
A4W93_RS01185 (A4W93_01165)	-	265439..266221 (+)	783	WP_085748869.1	Stp1/IreP family PP2C-type Ser/Thr phosphatase	-
A4W93_RS01190 (A4W93_01170)	-	266304..266987 (+)	684	WP_085748870.1	FHA domain-containing protein	-
A4W93_RS01195 (A4W93_01175)	-	267247..268023 (+)	777	WP_085748871.1	MBL fold metallo-hydrolase	-
A4W93_RS01200 (A4W93_01180)	pilA2	268234..268656 (+)	423	WP_085748872.1	pilin	Machinery gene
A4W93_RS30680 (A4W93_01185)	pilA	268719..269144 (+)	426	WP_085748873.1	pilin	Machinery gene
A4W93_RS01210 (A4W93_01190)	-	269141..270997 (+)	1857	WP_085748874.1	Wzy polymerase domain-containing protein	-
A4W93_RS01215 (A4W93_01195)	tfpZ	271095..271886 (+)	792	WP_085748875.1	TfpX/TfpZ family type IV pilin accessory protein	-

Sequence

Protein

• Length: 140 a.a.
• Molecular weight: 14410.29 Da
• Isoelectric Point: 7.7771

>NTDB_id=176837 A4W93_RS01200 WP_085748872.1 268234..268656(+) (pilA2) [Piscinibacter gummiphilus strain NS21]
MKRSIQKGFTLIELMIVVAIIGILAAVALPAYQDYVKRSRISEGLALASGAKTALAEYVASNNEWPASGSAAGIEDGTAI
NGNSVNSVTVGTSNGQITIEYASKAGGNNIVIQAATTAGGVTWDCRGGSVEGKYRPSECR

Nucleotide

• Length: 423 bp

>NTDB_id=176837 A4W93_RS01200 WP_085748872.1 268234..268656(+) (pilA2) [Piscinibacter gummiphilus strain NS21]
ATGAAGCGTTCCATCCAAAAGGGTTTCACCCTCATCGAACTGATGATCGTCGTCGCGATCATCGGCATCCTGGCCGCCGT
CGCTCTGCCGGCCTACCAGGACTACGTGAAGCGTTCGCGCATCTCTGAAGGCCTGGCGCTGGCTTCGGGTGCGAAGACCG
CCCTGGCCGAGTACGTCGCCTCGAACAACGAATGGCCGGCCAGCGGCTCCGCGGCTGGCATTGAAGATGGCACCGCCATC
AATGGCAACTCGGTGAACTCGGTCACGGTCGGTACGTCGAACGGTCAAATCACCATCGAGTACGCCAGCAAGGCCGGTGG
CAACAACATCGTCATTCAAGCTGCGACGACCGCCGGTGGCGTGACCTGGGACTGCCGCGGCGGCTCGGTCGAAGGCAAGT
ACCGCCCCAGCGAGTGCCGCTGA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (4-29)

• Pilin (bacterial filament) (Pilin) (38-140)

3D structure

• AlphaFold DB: A0A1W6L2Y2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA2	Legionella pneumophila str. Paris	54.412	97.143	0.529
pilA2	Legionella pneumophila strain ERS1305867	53.676	97.143	0.521
comP	Acinetobacter baylyi ADP1	46.528	100	0.479
pilA	Ralstonia pseudosolanacearum GMI1000	40	100	0.471
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	34.759	100	0.464
pilE	Neisseria gonorrhoeae MS11	39.623	100	0.45
pilE	Neisseria gonorrhoeae strain FA1090	39.873	100	0.45
pilA	Vibrio cholerae C6706	43.056	100	0.443
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	43.056	100	0.443
pilA	Vibrio cholerae strain A1552	43.056	100	0.443
pilA/pilA1	Eikenella corrodens VA1	37.908	100	0.414
pilA/pilAI	Pseudomonas stutzeri DSM 10701	39.86	100	0.407
pilA	Pseudomonas aeruginosa PAK	37.748	100	0.407
pilA	Vibrio campbellii strain DS40M4	35.484	100	0.393
pilA/pilAII	Pseudomonas stutzeri DSM 10701	38.298	100	0.386
pilA	Haemophilus influenzae 86-028NP	40.769	92.857	0.379