Detailed information NTG0160099

Bioinformatically predicted

Overview

• Name: pilA
• Type: Machinery gene
• Locus tag: DW68_RS26350
• Genome accession: NZ_CP013124
• Coordinates: 4125168..4125353 (-)
• Length: 61 a.a.
• NCBI ID: WP_017361392.1
• Uniprot ID: -
• Organism: Ectopseudomonas mendocina S5.2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4120168..4130353

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DW68_RS19220 (DW68_019215)	nadC	4121020..4121868 (+)	849	WP_017361387.1	carboxylating nicotinate-nucleotide diphosphorylase	-
DW68_RS19225 (DW68_019220)	-	4121976..4122887 (-)	912	WP_017361388.1	ABC transporter ATP-binding protein	-
DW68_RS19230 (DW68_019225)	-	4122890..4123735 (-)	846	WP_017361389.1	hypothetical protein	-
DW68_RS19235 (DW68_019230)	-	4123735..4124559 (-)	825	WP_231118620.1	hypothetical protein	-
DW68_RS25775	-	4124678..4125142 (-)	465	WP_155718711.1	hypothetical protein	-
DW68_RS26350 (DW68_019240)	pilA	4125168..4125353 (-)	186	WP_017361392.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
DW68_RS19250 (DW68_019245)	pilB	4125630..4127330 (+)	1701	WP_026041954.1	type IV-A pilus assembly ATPase PilB	Machinery gene
DW68_RS19255 (DW68_019250)	pilC	4127334..4128554 (+)	1221	WP_017361394.1	type II secretion system F family protein	Machinery gene
DW68_RS19260 (DW68_019255)	pilD	4128558..4129424 (+)	867	WP_172825344.1	A24 family peptidase	Machinery gene
DW68_RS19265 (DW68_019260)	coaE	4129694..4130302 (+)	609	WP_017361396.1	dephospho-CoA kinase	-

Sequence

Protein

• Length: 61 a.a.
• Molecular weight: 6703.99 Da
• Isoelectric Point: 10.5719

>NTDB_id=160099 DW68_RS26350 WP_017361392.1 4125168..4125353(-) (pilA) [Ectopseudomonas mendocina S5.2]
MKSLKKQKGFTLIELMIVIAIIGILAAIAIPQFNEYRQRANDTSTVADVKNNITSVIASLK

Nucleotide

• Length: 186 bp

>NTDB_id=160099 DW68_RS26350 WP_017361392.1 4125168..4125353(-) (pilA) [Ectopseudomonas mendocina S5.2]
ATGAAGTCTCTTAAAAAGCAAAAGGGTTTTACCCTGATCGAGCTGATGATCGTAATTGCCATTATCGGTATTCTGGCTGC
CATTGCTATTCCGCAGTTTAATGAATATCGCCAGCGCGCTAACGATACTTCTACAGTCGCTGATGTTAAGAATAACATTA
CTTCAGTTATTGCTTCTCTGAAGTAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (5-30)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilA	Pseudomonas aeruginosa PAK	59.649	93.443	0.557
pilA	Vibrio parahaemolyticus RIMD 2210633	64	81.967	0.525
pilA/pilA1	Eikenella corrodens VA1	75.61	67.213	0.508
pilA	Vibrio cholerae O1 biovar El Tor strain E7946	53.448	95.082	0.508
pilA	Vibrio cholerae strain A1552	53.448	95.082	0.508
pilA	Vibrio cholerae C6706	53.448	95.082	0.508
pilA2	Legionella pneumophila strain ERS1305867	66.667	73.77	0.492
pilA2	Legionella pneumophila str. Paris	66.667	73.77	0.492
pilA	Ralstonia pseudosolanacearum GMI1000	62.5	78.689	0.492
pilA	Acinetobacter nosocomialis M2	61.702	77.049	0.475
comP	Acinetobacter baylyi ADP1	75.676	60.656	0.459
pilV	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	57.143	80.328	0.459
pilE	Neisseria elongata subsp. glycolytica ATCC 29315	70	65.574	0.459
pilA/pilAI	Pseudomonas stutzeri DSM 10701	62.791	70.492	0.443
pilA/pilAII	Pseudomonas stutzeri DSM 10701	54	81.967	0.443
pilA	Acinetobacter baumannii strain A118	78.788	54.098	0.426
pilA/pilA4	Thermus thermophilus HB27	65	65.574	0.426
pilA	Vibrio campbellii strain DS40M4	72.222	59.016	0.426
pilE	Neisseria gonorrhoeae MS11	73.529	55.738	0.41
pilE	Neisseria gonorrhoeae strain FA1090	73.529	55.738	0.41
comF	Acinetobacter baylyi ADP1	63.158	62.295	0.393
pilA	Haemophilus influenzae 86-028NP	70.588	55.738	0.393
pilA	Haemophilus influenzae Rd KW20	70.588	55.738	0.393
pilA	Glaesserella parasuis strain SC1401	72.727	54.098	0.393
pilE	Acinetobacter baumannii D1279779	48.889	73.77	0.361
comE	Acinetobacter baylyi ADP1	59.459	60.656	0.361