Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   DW68_RS19255 Genome accession   NZ_CP013124
Coordinates   4127334..4128554 (+) Length   406 a.a.
NCBI ID   WP_017361394.1    Uniprot ID   -
Organism   Ectopseudomonas mendocina S5.2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4122334..4133554
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DW68_RS19230 (DW68_019225) - 4122890..4123735 (-) 846 WP_017361389.1 hypothetical protein -
  DW68_RS19235 (DW68_019230) - 4123735..4124559 (-) 825 WP_231118620.1 hypothetical protein -
  DW68_RS25775 - 4124678..4125142 (-) 465 WP_155718711.1 hypothetical protein -
  DW68_RS26350 (DW68_019240) pilA 4125168..4125353 (-) 186 WP_017361392.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DW68_RS19250 (DW68_019245) pilB 4125630..4127330 (+) 1701 WP_026041954.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DW68_RS19255 (DW68_019250) pilC 4127334..4128554 (+) 1221 WP_017361394.1 type II secretion system F family protein Machinery gene
  DW68_RS19260 (DW68_019255) pilD 4128558..4129424 (+) 867 WP_172825344.1 A24 family peptidase Machinery gene
  DW68_RS19265 (DW68_019260) coaE 4129694..4130302 (+) 609 WP_017361396.1 dephospho-CoA kinase -
  DW68_RS19270 (DW68_019265) yacG 4130299..4130493 (+) 195 WP_013714026.1 DNA gyrase inhibitor YacG -
  DW68_RS19275 (DW68_019270) - 4130566..4130781 (-) 216 WP_017361397.1 hypothetical protein -
  DW68_RS19280 (DW68_019275) - 4130887..4131576 (-) 690 WP_017361398.1 energy-coupling factor ABC transporter permease -
  DW68_RS19285 (DW68_019280) - 4131774..4132403 (+) 630 WP_017361399.1 DUF1780 domain-containing protein -
  DW68_RS19290 (DW68_019285) - 4132400..4132954 (+) 555 WP_017361400.1 MOSC domain-containing protein -
  DW68_RS19295 (DW68_019290) - 4133161..4133334 (+) 174 WP_013714021.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 406 a.a.        Molecular weight: 43979.61 Da        Isoelectric Point: 9.7131

>NTDB_id=160092 DW68_RS19255 WP_017361394.1 4127334..4128554(+) (pilC) [Ectopseudomonas mendocina S5.2]
MAEKALKTSVFAWEGTDRKGGKVKGELSGQSPALVKAQLRKQGINPIKVRKKAVSLFGAGKKIKPMDIALFTRQMATMMK
AGVPLLQSFDIIAEGFDNPNMRKLIDEVKQEVAAGNSFASALRKKPLYFDDLYCNLVDSGEQSGALETLLDRIATYKEKT
ESLKAKIKKAMTYPIAVIVVAVIVTAILLIKVVPQFESVFQGFGAELPAFTQVVVDLSRGLQNWWFVVLGGIFAAAFIFS
NTYKRSEKFRDALDRALLKAPIIGDILYKAVVARFARTLATTFAAGVPLVDALDSVAGATGNVVFRNAVHKIKVDVSSGT
QLNFAMRSTGIFPTMAVQMTAIGEEAGSLDEMLDKTASYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLIVAMYLPIFQ
LGAVVG

Nucleotide


Download         Length: 1221 bp        

>NTDB_id=160092 DW68_RS19255 WP_017361394.1 4127334..4128554(+) (pilC) [Ectopseudomonas mendocina S5.2]
ATGGCGGAAAAAGCACTGAAAACCAGCGTGTTCGCCTGGGAAGGCACCGATAGAAAGGGTGGCAAGGTAAAGGGCGAGCT
CAGTGGGCAATCGCCGGCCCTGGTCAAGGCTCAACTGCGCAAGCAGGGTATCAACCCGATCAAAGTGCGCAAGAAAGCGG
TTTCATTGTTTGGCGCGGGCAAGAAAATCAAGCCGATGGACATTGCCCTGTTCACCCGGCAAATGGCAACTATGATGAAG
GCCGGCGTACCGTTGTTGCAGTCCTTCGACATCATCGCTGAGGGTTTCGACAACCCCAACATGCGTAAGCTGATCGATGA
GGTTAAGCAGGAAGTTGCGGCCGGTAACAGCTTCGCTTCCGCACTACGCAAAAAACCGCTGTATTTCGACGACCTGTATT
GCAACCTGGTCGACTCAGGCGAACAGTCAGGCGCGCTGGAGACCTTGCTGGATCGTATTGCCACCTACAAGGAAAAGACC
GAATCCCTGAAAGCAAAGATCAAGAAGGCAATGACCTACCCGATTGCTGTGATCGTGGTCGCGGTCATCGTGACAGCCAT
TCTACTGATCAAGGTAGTACCTCAGTTCGAATCGGTATTCCAGGGTTTTGGTGCGGAGTTACCAGCCTTCACCCAAGTGG
TCGTAGACCTATCACGCGGCCTGCAAAATTGGTGGTTCGTGGTGCTGGGCGGGATATTCGCCGCCGCATTCATTTTTTCC
AACACATACAAGCGTTCGGAAAAATTTCGCGACGCTCTGGATAGGGCACTACTCAAGGCTCCAATCATTGGCGACATTCT
GTACAAGGCCGTGGTTGCACGCTTTGCCCGCACCCTCGCCACGACTTTTGCCGCTGGCGTGCCACTGGTCGACGCACTCG
ACTCGGTAGCCGGCGCCACCGGCAACGTGGTTTTCCGCAACGCCGTACACAAGATCAAAGTGGACGTGTCTTCGGGTACT
CAACTGAACTTCGCCATGCGCAGCACCGGCATCTTCCCCACTATGGCCGTGCAAATGACAGCTATCGGCGAAGAAGCCGG
CTCGCTGGACGAAATGCTTGATAAGACCGCCAGTTACTACGAAGAAGAAGTGGACAACGCCGTAGACAACCTCACTACCC
TGATGGAACCAATGATCATGGCTGTGCTTGGGGTACTGGTTGGTGGCCTTATCGTGGCGATGTATCTGCCTATCTTCCAG
CTGGGTGCGGTGGTCGGCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

80

99.754

0.798
  pilC Acinetobacter baylyi ADP1

61.823

100

0.618
  pilC Acinetobacter baumannii D1279779

60.494

99.754

0.603
  pilC Legionella pneumophila strain ERS1305867

53.535

97.537

0.522
  pilG Neisseria gonorrhoeae MS11

45.522

99.015

0.451
  pilG Neisseria meningitidis 44/76-A

45.522

99.015

0.451
  pilC Vibrio cholerae strain A1552

43.103

100

0.431
  pilC Vibrio campbellii strain DS40M4

41.709

98.03

0.409
  pilC Thermus thermophilus HB27

36.725

99.261

0.365

Multiple sequence alignment