Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   DW68_RS19250 Genome accession   NZ_CP013124
Coordinates   4125630..4127330 (+) Length   566 a.a.
NCBI ID   WP_026041954.1    Uniprot ID   -
Organism   Ectopseudomonas mendocina S5.2     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4120630..4132330
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  DW68_RS19220 (DW68_019215) nadC 4121020..4121868 (+) 849 WP_017361387.1 carboxylating nicotinate-nucleotide diphosphorylase -
  DW68_RS19225 (DW68_019220) - 4121976..4122887 (-) 912 WP_017361388.1 ABC transporter ATP-binding protein -
  DW68_RS19230 (DW68_019225) - 4122890..4123735 (-) 846 WP_017361389.1 hypothetical protein -
  DW68_RS19235 (DW68_019230) - 4123735..4124559 (-) 825 WP_231118620.1 hypothetical protein -
  DW68_RS25775 - 4124678..4125142 (-) 465 WP_155718711.1 hypothetical protein -
  DW68_RS26350 (DW68_019240) pilA 4125168..4125353 (-) 186 WP_017361392.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  DW68_RS19250 (DW68_019245) pilB 4125630..4127330 (+) 1701 WP_026041954.1 type IV-A pilus assembly ATPase PilB Machinery gene
  DW68_RS19255 (DW68_019250) pilC 4127334..4128554 (+) 1221 WP_017361394.1 type II secretion system F family protein Machinery gene
  DW68_RS19260 (DW68_019255) pilD 4128558..4129424 (+) 867 WP_172825344.1 A24 family peptidase Machinery gene
  DW68_RS19265 (DW68_019260) coaE 4129694..4130302 (+) 609 WP_017361396.1 dephospho-CoA kinase -
  DW68_RS19270 (DW68_019265) yacG 4130299..4130493 (+) 195 WP_013714026.1 DNA gyrase inhibitor YacG -
  DW68_RS19275 (DW68_019270) - 4130566..4130781 (-) 216 WP_017361397.1 hypothetical protein -
  DW68_RS19280 (DW68_019275) - 4130887..4131576 (-) 690 WP_017361398.1 energy-coupling factor ABC transporter permease -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62200.06 Da        Isoelectric Point: 5.3427

>NTDB_id=160091 DW68_RS19250 WP_026041954.1 4125630..4127330(+) (pilB) [Ectopseudomonas mendocina S5.2]
MNDSVIATGLARQLVNAELLDERAASNAQAQAARNKIPLVSYLVQNKLVSSRALAEVAAEQFGVPYFDLTAIDKESQPRE
VLSEKLCRQHRVLPLYRRGNKLFIALSDPTNHQALTDVQFSAGLSTEALLVEDDKLGDALERFFDTGNTGMEDLADVDLD
GLDVEAVTDDKDDAGSGGDADDAPVVRFINKMLLDAIKGGSSDLHFEPYEKTYRVRFRTDGMLHEVARPPIQLASRLSAR
LKVMAQLDISERRKPQDGRIKMKVSKNKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEGQKELYMTALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPKHGMDFSQALRAFLRQDPDVIMVGEIRD
LETAEIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCSCKKEVELPREALLKEGFPE
SQIGTFKLYAPNGCDNCKGGYKGRVGIYEVVKITPSLQRIIMEEGNSIEIAKQARADGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=160091 DW68_RS19250 WP_026041954.1 4125630..4127330(+) (pilB) [Ectopseudomonas mendocina S5.2]
ATGAACGATTCCGTAATCGCAACCGGCTTGGCCCGACAGCTCGTCAACGCAGAATTGCTCGACGAACGTGCAGCCAGCAA
CGCTCAAGCCCAGGCTGCGCGTAACAAGATTCCGCTGGTGTCGTATCTGGTACAGAACAAGCTGGTAAGCAGCCGCGCAC
TGGCAGAGGTAGCAGCCGAGCAATTTGGCGTTCCGTACTTCGACCTGACCGCCATCGACAAGGAGAGCCAACCTCGCGAG
GTGCTGAGCGAAAAGCTCTGCCGGCAGCATCGGGTGCTACCGCTATACCGCCGGGGCAACAAGCTGTTTATCGCCCTCTC
CGACCCGACCAATCACCAGGCGCTGACCGATGTTCAATTCAGTGCCGGACTTAGCACAGAAGCCCTGCTGGTCGAGGACG
ACAAACTGGGCGATGCCTTGGAGCGTTTTTTCGATACTGGCAACACCGGCATGGAGGATTTGGCCGATGTCGATCTGGAT
GGCCTGGATGTAGAGGCCGTTACCGACGACAAGGACGATGCCGGCTCTGGCGGCGATGCTGATGACGCACCGGTGGTGCG
CTTTATCAACAAGATGCTGCTGGATGCGATCAAGGGCGGCTCATCCGACCTGCACTTCGAACCCTACGAGAAAACCTATC
GCGTGCGCTTTCGTACCGACGGTATGTTGCACGAGGTGGCACGCCCGCCAATCCAACTGGCCAGTCGCCTGTCTGCTCGC
CTAAAGGTCATGGCGCAACTGGATATCTCCGAGCGACGCAAGCCGCAGGATGGCCGGATCAAGATGAAGGTATCGAAGAA
CAAGGCCATCGACTTTCGCGTAAACACCCTGCCCACCCTGTGGGGCGAAAAAATCGTGATGCGGATTCTCGACCCTACCA
GCGCCCAAATGGGCATCGATGCCCTGGGTTACGAGGAGGGTCAGAAAGAGCTGTATATGACAGCCCTGAAGCAACCGCAG
GGGATGATTCTGGTAACCGGCCCTACCGGCTCGGGCAAGACGGTATCGCTCTATACCGGGCTGAATATTCTCAATACGCC
GGATATCAATATCTCCACTGCAGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCCAAGC
ATGGCATGGACTTCTCGCAGGCTCTGCGCGCGTTCCTGCGTCAGGATCCAGACGTGATCATGGTCGGCGAGATTCGCGAT
CTGGAAACCGCCGAAATCGCGGTAAAGGCCGCGCAAACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCGGC
GGAAACCCTGACACGTCTACGCAATATGGGCGTACCAGCATTCAATATCGCCACCTCGGTCAACCTGATCATCGCTCAGC
GCCTGGCGCGCAAACTCTGTAGCTGCAAGAAAGAAGTAGAACTGCCGCGCGAGGCTTTACTCAAGGAAGGCTTCCCCGAG
AGCCAGATTGGCACCTTCAAGCTTTACGCTCCCAACGGCTGCGATAATTGCAAAGGCGGCTATAAAGGAAGGGTGGGGAT
TTATGAAGTAGTTAAAATCACGCCCTCGCTGCAACGCATTATCATGGAGGAAGGCAACTCCATCGAAATCGCCAAGCAGG
CGCGAGCCGATGGTTTCAACGACCTCCGTACTTCAGGGCTGCTCAAGGCGATGCAGGGCATCACCAGCCTGGAAGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.503

99.47

0.592
  pilB Acinetobacter baylyi ADP1

58.541

99.293

0.581
  pilB Legionella pneumophila strain ERS1305867

54.529

99.47

0.542
  pilB Vibrio cholerae strain A1552

50.796

99.823

0.507
  pilF Neisseria gonorrhoeae MS11

49.648

100

0.498
  pilB Vibrio parahaemolyticus RIMD 2210633

51.577

95.23

0.491
  pilB Vibrio campbellii strain DS40M4

48.929

98.94

0.484
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.962

93.286

0.373
  pilF Thermus thermophilus HB27

39.623

93.64

0.371

Multiple sequence alignment