Detailed information NTG0160093

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: DW68_RS19260
• Genome accession: NZ_CP013124
• Coordinates: 4128558..4129424 (+)
• Length: 288 a.a.
• NCBI ID: WP_172825344.1
• Uniprot ID: -
• Organism: Ectopseudomonas mendocina S5.2
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4123558..4134424

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
DW68_RS19235 (DW68_019230)	-	4123735..4124559 (-)	825	WP_231118620.1	hypothetical protein	-
DW68_RS25775	-	4124678..4125142 (-)	465	WP_155718711.1	hypothetical protein	-
DW68_RS26350 (DW68_019240)	pilA	4125168..4125353 (-)	186	WP_017361392.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
DW68_RS19250 (DW68_019245)	pilB	4125630..4127330 (+)	1701	WP_026041954.1	type IV-A pilus assembly ATPase PilB	Machinery gene
DW68_RS19255 (DW68_019250)	pilC	4127334..4128554 (+)	1221	WP_017361394.1	type II secretion system F family protein	Machinery gene
DW68_RS19260 (DW68_019255)	pilD	4128558..4129424 (+)	867	WP_172825344.1	A24 family peptidase	Machinery gene
DW68_RS19265 (DW68_019260)	coaE	4129694..4130302 (+)	609	WP_017361396.1	dephospho-CoA kinase	-
DW68_RS19270 (DW68_019265)	yacG	4130299..4130493 (+)	195	WP_013714026.1	DNA gyrase inhibitor YacG	-
DW68_RS19275 (DW68_019270)	-	4130566..4130781 (-)	216	WP_017361397.1	hypothetical protein	-
DW68_RS19280 (DW68_019275)	-	4130887..4131576 (-)	690	WP_017361398.1	energy-coupling factor ABC transporter permease	-
DW68_RS19285 (DW68_019280)	-	4131774..4132403 (+)	630	WP_017361399.1	DUF1780 domain-containing protein	-
DW68_RS19290 (DW68_019285)	-	4132400..4132954 (+)	555	WP_017361400.1	MOSC domain-containing protein	-
DW68_RS19295 (DW68_019290)	-	4133161..4133334 (+)	174	WP_013714021.1	DUF3094 family protein	-

Sequence

Protein

• Length: 288 a.a.
• Molecular weight: 31858.75 Da
• Isoelectric Point: 5.6608

>NTDB_id=160093 DW68_RS19260 WP_172825344.1 4128558..4129424(+) (pilD) [Ectopseudomonas mendocina S5.2]
MALEIMADNLSAFVFICVVFGLLIGSFLNVVVYRLPLMLQRDWQSQAREVLELPAAPTTETFNLILPNSSCPNCGHEIRP
WENIPVISYLFLRGKCSNCKTPISKRYPIVELACGLLSGYVAWHFGFTWQAGAMLVFAWGLLAMSLIDADHQILPDVLVL
PLLWLGLIVNYYGLFTSLEDALWGAIAGYLSLWSVYWLFKLITGKEGMGYGDFKLLAMLGAWGGWQILPLTILLSSLVGA
VLGVILLRLRGSDSSTPIPFGPYLAIAGWIALLWGDVITSNYLKFAGF

Nucleotide

• Length: 867 bp

>NTDB_id=160093 DW68_RS19260 WP_172825344.1 4128558..4129424(+) (pilD) [Ectopseudomonas mendocina S5.2]
ATGGCGCTCGAAATTATGGCCGACAATCTGTCGGCCTTTGTTTTTATCTGTGTAGTTTTTGGCCTGCTAATCGGCAGCTT
CCTCAACGTCGTGGTCTACCGCCTACCGCTGATGCTGCAACGCGACTGGCAAAGCCAAGCGCGTGAAGTATTGGAACTAC
CAGCGGCGCCGACAACAGAAACTTTCAACCTGATACTCCCCAACTCCTCATGCCCCAACTGCGGCCATGAGATTCGGCCC
TGGGAAAACATTCCGGTGATCAGCTACCTGTTCCTGCGCGGCAAGTGCTCGAACTGCAAGACGCCGATCAGCAAGCGCTA
CCCTATCGTAGAATTGGCCTGCGGCCTGCTTTCAGGCTATGTCGCCTGGCACTTCGGTTTTACCTGGCAGGCCGGAGCGA
TGCTGGTGTTTGCCTGGGGGCTGCTGGCCATGAGTCTGATCGATGCTGATCACCAGATTCTGCCGGATGTGCTGGTGCTG
CCGCTGCTTTGGCTGGGCTTGATCGTCAATTACTACGGGTTGTTCACCAGCCTGGAAGATGCCTTGTGGGGGGCGATCGC
CGGTTATCTGAGCCTATGGTCGGTGTACTGGCTATTCAAGCTGATCACCGGCAAGGAAGGCATGGGTTACGGTGACTTCA
AGCTGCTGGCCATGCTTGGCGCCTGGGGTGGCTGGCAGATTCTGCCACTGACCATTCTGCTGTCGTCGCTGGTCGGGGCG
GTGCTGGGTGTGATTCTGCTGCGCCTGCGTGGCAGCGACTCCAGCACGCCGATTCCCTTCGGGCCCTATCTGGCGATTGC
AGGGTGGATCGCCTTGCTTTGGGGTGATGTGATTACTAGTAACTATCTGAAGTTCGCTGGGTTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (135-244)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (19-125)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Acinetobacter baumannii D1279779	53.237	96.528	0.514
pilD	Vibrio cholerae strain A1552	53.65	95.139	0.51
pilD	Acinetobacter nosocomialis M2	52.878	96.528	0.51
pilD	Vibrio campbellii strain DS40M4	53.846	94.792	0.51
pilD	Neisseria gonorrhoeae MS11	49.077	94.097	0.462